Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,045,836 T→A 25.7% intergenic (+598/+197) yqiK → / ← rfaE conserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,045,8360TA25.7% 238.3 / 76.8 113intergenic (+598/+197)yqiK/rfaEconserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase
Reads supporting (aligned to +/- strand):  ref base T (33/51);  new base A (14/15);  total (47/66)
Fisher's exact test for biased strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 113 total aligned reads displayed.

GCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGA  >  Exported/2045772‑2045905
                                                                 |                                                                     
gcTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGta                                                                   >  1:5442407/1‑69 (MQ=255)
 cTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGtata                                                                 <  1:1887124/70‑1 (MQ=255)
    atcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGtat                                                                  <  1:2333700/66‑1 (MQ=255)
    atcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                              >  1:3343442/1‑70 (MQ=255)
     tcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                     <  1:4827154/62‑1 (MQ=255)
     tcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                     <  1:1552405/62‑1 (MQ=255)
     tcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGaa                                                            <  1:2717963/71‑1 (MQ=255)
       aacaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAg                                                          >  1:2466718/1‑71 (MQ=255)
       aacaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAg                                                          >  1:5640262/1‑71 (MQ=255)
        ctaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGaaa                                                           <  1:4132819/67‑1 (MQ=255)
        acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                     <  1:711585/59‑1 (MQ=255)
        acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                     <  1:1271467/59‑1 (MQ=255)
        acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                     <  1:5090951/59‑1 (MQ=255)
                  ggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                              <  1:276982/56‑1 (MQ=255)
                  ggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                              <  1:346786/56‑1 (MQ=255)
                   gcagcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                              <  1:165441/55‑1 (MQ=255)
                   gcagcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGa                                                             <  1:733399/56‑1 (MQ=255)
                    cagcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAg                                             >  1:830053/1‑71 (MQ=255)
                     agcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTa                                                     <  1:2531398/62‑1 (MQ=255)
                     agcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTa                                                     <  1:4329956/62‑1 (MQ=255)
                      gcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                              <  1:4661619/52‑1 (MQ=255)
                      gcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAgg                                                         <  1:3000704/57‑1 (MQ=255)
                      gcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTatg                                                   >  1:4328361/1‑63 (MQ=255)
                        aaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGt                                                       >  1:781119/1‑57 (MQ=255)
                         aGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTa                                                     >  1:3736880/1‑58 (MQ=255)
                         aGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc                                          <  1:657273/69‑1 (MQ=255)
                           gggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGTCCTTGTATAAGGAAAGGGTTa                                                     <  1:1977594/56‑1 (MQ=255)
                           acgCTGAAACGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGt                                        >  1:303292/3‑70 (MQ=0)
                            ggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat                                     >  1:3187602/1‑71 (MQ=255)
                            ggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat                                     >  1:5627124/1‑71 (MQ=255)
                            ggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat                                     >  1:552372/1‑71 (MQ=255)
                            cgCTGAAACGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGtca                                      <  1:1786500/70‑1 (MQ=1)
                              cTGAAACGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGaaa                                                           >  1:3653992/1‑47 (MQ=14)
                              cTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATaa                                                                >  1:442617/1‑42 (MQ=255)
                                gAAACGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGaa                                                            >  1:1492151/1‑44 (MQ=14)
                                gAAACGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACtcat                                     >  1:1896669/1‑67 (MQ=14)
                                gAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGtt                                                      <  1:1190765/50‑1 (MQ=255)
                                 aaaCGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                 <  1:3787364/54‑1 (MQ=14)
                                 aaaCGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcatcg                                <  1:565443/71‑3 (MQ=11)
                                 aaaCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc                                >  1:4899675/1‑71 (MQ=255)
                                  aaCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAg                                             >  1:3535738/1‑57 (MQ=255)
                                   aCGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCAtgatga                                               >  1:3633700/1‑54 (MQ=14)
                                   aCGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCAtgatga                                               >  1:3590871/1‑54 (MQ=14)
                                   aCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAATGAAAGGGTTATGATGAAGCTCGt                                        <  1:5193989/61‑1 (MQ=255)
                                      ggAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                 <  1:2622584/49‑1 (MQ=14)
                                      ggAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtg                           >  1:1155474/1‑70 (MQ=2)
                                      ggAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgtt                           >  1:3793091/1‑71 (MQ=255)
                                       gAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgt                            >  1:3680797/1‑70 (MQ=11)
                                       gAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtgc                          >  1:3332284/1‑69 (MQ=2)
                                       gAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttg                          <  1:2081641/71‑1 (MQ=255)
                                        aaaGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtgc                          >  1:3132334/1‑68 (MQ=2)
                                        aaaGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtgc                          >  1:4164927/1‑68 (MQ=2)
                                          aGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGt                                                       <  1:3505689/40‑1 (MQ=0)
                                          aGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGt                                                       <  1:22867/40‑1 (MQ=0)
                                             cccTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACt                               <  1:1253870/60‑1 (MQ=255)
                                              tcTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtg                           <  1:3123410/63‑2 (MQ=11)
                                              ccTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt                    >  1:3907027/1‑70 (MQ=255)
                                               cTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc                                       <  1:1663603/50‑1 (MQ=255)
                                               cTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc                                       <  1:3373786/50‑1 (MQ=255)
                                                tCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCat                                                    <  1:1943990/36‑1 (MQ=12)
                                                tCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCat                                                    <  1:128161/36‑1 (MQ=12)
                                                tCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCat                                                    <  1:2965762/36‑1 (MQ=12)
                                                 cccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCtac                                         >  1:74665/1‑44 (MQ=9)
                                                 cccGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGc                                            >  1:3127908/1‑43 (MQ=255)
                                                 cccGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa                                 >  1:4898656/1‑54 (MQ=255)
                                                  ccGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtga                          >  1:3161910/1‑59 (MQ=2)
                                                  ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCatga                                                  >  1:4550644/1‑36 (MQ=12)
                                                  ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCatga                                                  >  1:5472944/1‑36 (MQ=12)
                                                  ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCatga                                                  >  1:1660542/1‑36 (MQ=12)
                                                  ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat                                  <  1:2184869/52‑1 (MQ=9)
                                                  ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat                                  <  1:213885/52‑1 (MQ=9)
                                                  ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat                                  <  1:3479904/52‑1 (MQ=9)
                                                  ccGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt                    <  1:2337461/66‑1 (MQ=255)
                                                   cGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                 <  1:3931266/36‑1 (MQ=9)
                                                   cGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                 <  1:2218638/36‑1 (MQ=9)
                                                   cGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa                                 >  1:1020149/1‑52 (MQ=255)
                                                   cGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg                              <  1:2294832/55‑1 (MQ=255)
                                                    gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa                                 >  1:525181/1‑51 (MQ=255)
                                                    gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt             <  1:4698006/71‑1 (MQ=255)
                                                    gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt             <  1:4307788/71‑1 (MQ=255)
                                                    gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGt              >  1:5507266/1‑70 (MQ=255)
                                                     aagaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgt                            >  1:1201610/1‑55 (MQ=255)
                                                     aagaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt             >  1:3440667/1‑70 (MQ=255)
                                                     aagaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa            <  1:2702621/71‑1 (MQ=255)
                                                     aagaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa            <  1:1849759/71‑1 (MQ=255)
                                                      agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg                             >  1:3598080/1‑53 (MQ=255)
                                                      agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg                             >  1:4303137/1‑53 (MQ=255)
                                                      agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg                             >  1:611677/1‑53 (MQ=255)
                                                      agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa           <  1:4262499/71‑1 (MQ=255)
                                                      agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa           <  1:1283014/71‑1 (MQ=255)
                                                       gaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg          >  1:5332259/1‑71 (MQ=255)
                                                          ggggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg                     <  1:5080620/57‑1 (MQ=255)
                                                            gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg                     <  1:4397732/56‑1 (MQ=255)
                                                            gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa           <  1:2402884/66‑1 (MQ=255)
                                                            gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa           <  1:5556436/66‑1 (MQ=255)
                                                              gCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc    <  1:2599328/71‑1 (MQ=255)
                                                               ccTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg                              <  1:5123806/44‑1 (MQ=255)
                                                                cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa  >  1:5610479/1‑71 (MQ=255)
                                                                cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa  >  1:4961894/1‑71 (MQ=255)
                                                                cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa  >  1:2174414/1‑71 (MQ=255)
                                                                 |                                                                     
GCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGA  >  Exported/2045772‑2045905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: