Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,068,650 G→T 15.1% G380G (GGC→GGA aer ← fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,068,6500GT15.1% 182.5 / 14.4 67G380G (GGC→GGAaerfused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base G (33/22);  new base T (5/5);  total (39/28)
Fisher's exact test for biased strand distribution p-value = 7.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

ATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCA  >  Exported/2068584‑2068720
                                                                  |                                                                      
aTTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCgg                                                                    <  1:1934680/71‑1 (MQ=255)
 ttAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGcc                                                                      <  1:2562609/68‑1 (MQ=255)
 ttAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCgg                                                                    <  1:4988295/70‑1 (MQ=255)
  tAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCa                                                                  >  1:4627060/1‑71 (MQ=255)
   aGCGCTGCGGCTGGCTAAATGCCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCg                                                                     >  1:3394627/1‑67 (MQ=255)
   aGCGCTGCGGCTGGCTAAATGCCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAc                                                                 >  1:1497492/1‑71 (MQ=255)
   aGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCg                                                                     >  1:288336/1‑67 (MQ=255)
     cgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAcgc                                                               <  1:2795825/71‑1 (MQ=255)
      gcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAcgcg                                                              <  1:3653829/71‑1 (MQ=255)
       cTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACgcgc                                                             <  1:31476/71‑1 (MQ=255)
        tacGGCTGGCAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCgagc                                                             >  1:949004/3‑70 (MQ=255)
          cGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGc                                                                       >  1:2561294/1‑58 (MQ=255)
             ctggctAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCGTc                                                       >  1:5514198/1‑71 (MQ=255)
                     aTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGc                                                                       >  1:3498637/1‑47 (MQ=255)
                      tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAccgccg                                               >  1:2579055/1‑70 (MQ=255)
                      tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAccgcc                                                >  1:4293917/1‑69 (MQ=255)
                        aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc                                                            <  1:5137532/55‑1 (MQ=255)
                        aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc                                                            <  1:3152915/55‑1 (MQ=255)
                        aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc                                                            <  1:3223092/55‑1 (MQ=255)
                         cGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt                                           <  1:4839485/71‑1 (MQ=255)
                              ttCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAggg                                      >  1:4406751/1‑71 (MQ=255)
                               tcaccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCa                                         >  1:3808880/4‑67 (MQ=255)
                               tcaccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGc                                     >  1:1607488/4‑71 (MQ=255)
                               tCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCa                                                     >  1:5501552/1‑55 (MQ=255)
                               tCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCa                                                     >  1:3851941/1‑55 (MQ=255)
                                ccccTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGc                                     >  1:3133454/1‑70 (MQ=255)
                                  ccTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAg                                  <  1:3115023/71‑1 (MQ=255)
                                    tGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt                                           <  1:1804907/60‑1 (MQ=255)
                                    tGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa                                >  1:4876798/1‑71 (MQ=255)
                                     gCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGaa                                >  1:551201/1‑70 (MQ=255)
                                     gCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa                                >  1:3128799/1‑70 (MQ=255)
                                      ccactaCCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGaa                                <  1:1596229/69‑1 (MQ=255)
                                      ccaccaCTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAc                                                    >  1:1004843/1‑49 (MQ=255)
                                       caccacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat                             <  1:3381062/71‑1 (MQ=255)
                                       caccacAGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat                             >  1:1366067/1‑71 (MQ=255)
                                         ccacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTg                           <  1:3391711/71‑1 (MQ=255)
                                         ccacCGCAAAACCGCGCCCTTGCTCACCCGCACGCGCAGCCTCAACCGCCGCGTTCAAAGCCAGAATATTg                           >  1:2145271/1‑71 (MQ=255)
                                         ccacCGCAAAACCGCGCCCTTGCTCACCCGCACGCGCAGCCTCAACCGCCGCGTTCAAAGCCAGAATATTg                           <  1:5665247/71‑1 (MQ=255)
                                           acTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCa                                         >  1:872275/1‑55 (MQ=255)
                                           acTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGt                         >  1:1418717/1‑71 (MQ=255)
                                            cTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTc                                                       <  1:983823/40‑1 (MQ=255)
                                            cTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTc                        >  1:4854925/1‑71 (MQ=255)
                                               cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAg                                        >  1:560931/1‑52 (MQ=255)
                                               cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTg                      <  1:3944400/70‑1 (MQ=255)
                                                aaaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata                              >  1:915605/1‑61 (MQ=255)
                                                aaaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata                              >  1:5113625/1‑61 (MQ=255)
                                                  aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTc                                          <  1:4460276/47‑1 (MQ=255)
                                                  aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc                  <  1:3913188/71‑1 (MQ=255)
                                                  aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc                  <  1:210974/71‑1 (MQ=255)
                                                  aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc                  <  1:310011/71‑1 (MQ=255)
                                                  aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc                  >  1:2298716/1‑71 (MQ=255)
                                                    ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACg                 >  1:2514079/1‑70 (MQ=255)
                                                    ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc                <  1:1503705/71‑1 (MQ=255)
                                                    ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc                <  1:61465/71‑1 (MQ=255)
                                                     cTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCa               <  1:3548251/71‑1 (MQ=255)
                                                        ggCCCTGCTCGCCGGCGCGCGCCGCTTCGACcgccgc                                              >  1:1004657/2‑37 (MQ=25)
                                                          cccTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAattcc          <  1:2684443/71‑4 (MQ=255)
                                                           ccTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCg         >  1:5478507/1‑71 (MQ=255)
                                                            cTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt                                           <  1:74979/36‑1 (MQ=16)
                                                             tGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGt        >  1:5684660/1‑70 (MQ=255)
                                                              gTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGtt       >  1:4153744/1‑70 (MQ=255)
                                                               ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATAtt                            >  1:4971986/1‑48 (MQ=255)
                                                               ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATAtt                            >  1:1426970/1‑48 (MQ=255)
                                                               ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTaa     >  1:3250491/1‑71 (MQ=255)
                                                               ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTaa     <  1:3532087/71‑1 (MQ=255)
                                                                 cGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCATTGATc   >  1:2974824/1‑71 (MQ=255)
                                                                 cGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCATTGATc   >  1:5670102/1‑71 (MQ=255)
                                                                  gCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCa  <  1:4874683/71‑1 (MQ=255)
                                                                  |                                                                      
ATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCA  >  Exported/2068584‑2068720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: