Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,068,659 C→A 16.9% A377A (GCG→GCT aer ← fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,068,6590CA16.9% 156.9 / 14.4 59A377A (GCG→GCTaerfused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base C (30/19);  new base A (5/5);  total (35/24)
Fisher's exact test for biased strand distribution p-value = 7.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCG  >  Exported/2068589‑2068723
                                                                      |                                                                
cgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAcgc                                                                  <  1:2795825/71‑1 (MQ=255)
 gcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAcgcg                                                                 <  1:3653829/71‑1 (MQ=255)
  cTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACgcgc                                                                <  1:31476/71‑1 (MQ=255)
   tacGGCTGGCAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCgagc                                                                >  1:949004/3‑70 (MQ=255)
        ctggctAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCGTc                                                          >  1:5514198/1‑71 (MQ=255)
                 tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAccgccg                                                  >  1:2579055/1‑70 (MQ=255)
                 tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAccgcc                                                   >  1:4293917/1‑69 (MQ=255)
                   aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc                                                               <  1:3152915/55‑1 (MQ=255)
                   aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc                                                               <  1:3223092/55‑1 (MQ=255)
                   aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc                                                               <  1:5137532/55‑1 (MQ=255)
                    cGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt                                              <  1:4839485/71‑1 (MQ=255)
                         ttCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAggg                                         >  1:4406751/1‑71 (MQ=255)
                          tcaccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCa                                            >  1:3808880/4‑67 (MQ=255)
                          tcaccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGc                                        >  1:1607488/4‑71 (MQ=255)
                          tCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCa                                                        >  1:3851941/1‑55 (MQ=255)
                          tCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCa                                                        >  1:5501552/1‑55 (MQ=255)
                           ccccTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGc                                        >  1:3133454/1‑70 (MQ=255)
                             ccTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAg                                     <  1:3115023/71‑1 (MQ=255)
                               tGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt                                              <  1:1804907/60‑1 (MQ=255)
                               tGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa                                   >  1:4876798/1‑71 (MQ=255)
                                gCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGaa                                   >  1:551201/1‑70 (MQ=255)
                                gCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa                                   >  1:3128799/1‑70 (MQ=255)
                                 ccactaCCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGaa                                   <  1:1596229/69‑1 (MQ=255)
                                 ccaccaCTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAc                                                       >  1:1004843/1‑49 (MQ=255)
                                  caccacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat                                <  1:3381062/71‑1 (MQ=255)
                                  caccacAGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat                                >  1:1366067/1‑71 (MQ=255)
                                    ccacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTg                              <  1:3391711/71‑1 (MQ=255)
                                    ccacCGCAAAACCGCGCCCTTGCTCACCCGCACGCGCAGCCTCAACCGCCGCGTTCAAAGCCAGAATATTg                              <  1:5665247/71‑1 (MQ=255)
                                    ccacCGCAAAACCGCGCCCTTGCTCACCCGCACGCGCAGCCTCAACCGCCGCGTTCAAAGCCAGAATATTg                              >  1:2145271/1‑71 (MQ=255)
                                      acTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCa                                            >  1:872275/1‑55 (MQ=255)
                                      acTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGt                            >  1:1418717/1‑71 (MQ=255)
                                       cTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTc                                                          <  1:983823/40‑1 (MQ=255)
                                       cTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTc                           >  1:4854925/1‑71 (MQ=255)
                                          cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAg                                           >  1:560931/1‑52 (MQ=255)
                                          cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTg                         <  1:3944400/70‑1 (MQ=255)
                                           aaaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata                                 >  1:915605/1‑61 (MQ=255)
                                           aaaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata                                 >  1:5113625/1‑61 (MQ=255)
                                             aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTc                                             <  1:4460276/47‑1 (MQ=255)
                                             aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc                     <  1:210974/71‑1 (MQ=255)
                                             aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc                     <  1:310011/71‑1 (MQ=255)
                                             aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc                     >  1:2298716/1‑71 (MQ=255)
                                             aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc                     <  1:3913188/71‑1 (MQ=255)
                                               ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACg                    >  1:2514079/1‑70 (MQ=255)
                                               ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc                   <  1:61465/71‑1 (MQ=255)
                                               ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc                   <  1:1503705/71‑1 (MQ=255)
                                                cTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCa                  <  1:3548251/71‑1 (MQ=255)
                                                   ggCCCTGCTCGCCGGCGCGCGCCGCTTCGACcgccgc                                                 >  1:1004657/2‑37 (MQ=25)
                                                     cccTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAattcc             <  1:2684443/71‑4 (MQ=255)
                                                      ccTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCg            >  1:5478507/1‑71 (MQ=255)
                                                       cTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt                                              <  1:74979/36‑1 (MQ=16)
                                                        tGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGt           >  1:5684660/1‑70 (MQ=255)
                                                         gTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGtt          >  1:4153744/1‑70 (MQ=255)
                                                          ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATAtt                               >  1:4971986/1‑48 (MQ=255)
                                                          ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATAtt                               >  1:1426970/1‑48 (MQ=255)
                                                          ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTaa        >  1:3250491/1‑71 (MQ=255)
                                                          ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTaa        <  1:3532087/71‑1 (MQ=255)
                                                            cGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCATTGATc      >  1:2974824/1‑71 (MQ=255)
                                                            cGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCATTGATc      >  1:5670102/1‑71 (MQ=255)
                                                             gCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCa     <  1:4874683/71‑1 (MQ=255)
                                                              ccGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAg    <  1:265479/71‑1 (MQ=255)
                                                               cGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCt                          >  1:3075071/1‑48 (MQ=38)
                                                                ggCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCg  <  1:3486042/71‑1 (MQ=255)
                                                                ggCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCg  <  1:1418521/71‑1 (MQ=255)
                                                                ggCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCg  <  1:4441120/71‑1 (MQ=255)
                                                                 gCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAatat              >  1:4307586/1‑58 (MQ=255)
                                                                   aCGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCg  >  1:4356910/1‑68 (MQ=255)
                                                                      |                                                                
CGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAATCAGCG  >  Exported/2068589‑2068723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: