Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,093,640	T→C	8.5%	V404V (GTA→GTG)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,093,640	0	T	C	8.5%	227.0 / 8.4	82	V404V (GTA→GTG)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base T (40/35);  new base C (5/2);  total (45/37)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTCGATCACTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCG  >  Exported/2093573‑2093709                                                                    |                                                                      tctcGATCACTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACt                                                                    <  1:2729347/71‑1 (MQ=255)   tcGATCACTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGc                                                                  >  1:4391367/1‑71 (MQ=255)   tcGATCACTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGc                                                                  >  1:445518/1‑71 (MQ=255)   tcGATAACTTTATCGAGGCAGACGCGCGGCTCGCTGGTACGGCGCAGCGCCATACGTGCGGCGTTCACCGc                                                                  >  1:2306192/1‑71 (MQ=255)   tcGATAACTTTATCGAGGCAGACGCGCGGCTCGCTGGTACGGCGCAGCGCCATACGTGCGGCGTTCACCGc                                                                  >  1:2839258/1‑71 (MQ=255)   tcGATAACTTTATCGAGGCAGACGCGCGGCTCGCTGGTACGGCGCAGCGCCATACGTGCGGCGTTCACCGc                                                                  >  1:2832423/1‑71 (MQ=255)      aTCACTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCttt                                                               <  1:1398546/71‑1 (MQ=255)        cACTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCa                                                             >  1:5092501/1‑71 (MQ=255)         aCTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCa                                                             <  1:3998911/70‑1 (MQ=255)         aCTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCAc                                                            >  1:1138929/1‑71 (MQ=255)         aCTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCAc                                                            >  1:5156758/1‑71 (MQ=255)         aCTTTATCGAGGCAGACGCGCGGCTCGCTGGTACGGCGCAGCGCCATACGTGCGGCGTTCACCGCtttta                                                             >  1:2238903/1‑68 (MQ=255)         aCTTTATCGAGGCAGACGCGCGGCTCGCTGGTACGGCGCAGCGCCATACGTGCGGCGTTCACCGCtttta                                                             >  1:5467703/1‑68 (MQ=255)             tATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTa                                                                      >  1:571797/1‑57 (MQ=255)                 gAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTaa                                                    <  1:5292513/71‑1 (MQ=255)                 gAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTa                                                     <  1:4742627/70‑1 (MQ=255)                 gAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTa                                                     <  1:1870467/70‑1 (MQ=255)                   gTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCAtt                                                      <  1:5099267/67‑1 (MQ=255)                   gTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATg                                                  <  1:1792541/71‑1 (MQ=255)                   gTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATg                                                  <  1:2313355/71‑1 (MQ=255)                    tGAAAAACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATgg                                                 >  1:5601664/1‑71 (MQ=255)                     gAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCAtt                                                      <  1:2256451/65‑1 (MQ=255)                      aaaCACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACg                                                           >  1:2336679/1‑59 (MQ=255)                      aaaCACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCa                                               >  1:5084048/1‑71 (MQ=255)                       aaCACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCAt                                              >  1:4501693/1‑71 (MQ=255)                        acacGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCAt                                              >  1:2520186/1‑70 (MQ=255)                          acGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTAc                                           >  1:1049574/1‑71 (MQ=255)                          acGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTAc                                           >  1:4586301/1‑71 (MQ=255)                              ggTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                       <  1:4699382/71‑1 (MQ=255)                              ggTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                       <  1:1307244/71‑1 (MQ=255)                               gTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                       >  1:1051328/1‑70 (MQ=255)                               gTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                       <  1:109478/70‑1 (MQ=255)                                tGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                     <  1:4429569/71‑1 (MQ=255)                                  ccGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATg                                   >  1:2188227/1‑71 (MQ=255)                                   cGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACCGCt                                                                 <  1:1971276/40‑1 (MQ=255)                                     aGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCAc                                >  1:3772774/1‑71 (MQ=255)                                     aGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCAc                                >  1:3369357/1‑71 (MQ=255)                                       gTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCAtt                                                      <  1:662829/47‑1 (MQ=255)                                         gcggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGa                            >  1:3882536/1‑71 (MQ=255)                                         gcggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGa                            >  1:2599924/1‑71 (MQ=255)                                          cggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCa                                                        >  1:3578765/1‑42 (MQ=255)                                               cATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGt                       <  1:1185664/70‑1 (MQ=255)                                                aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                       <  1:1292180/53‑1 (MQ=255)                                                aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                     >  1:5358735/1‑71 (MQ=255)                                                 tCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTaa                                                    >  1:4537388/1‑39 (MQ=255)                                                 tCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                     >  1:759590/1‑54 (MQ=255)                                                 tCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                     >  1:5364519/1‑54 (MQ=255)                                                   gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTa                                                     >  1:3830431/1‑36 (MQ=255)                                                   gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAAt                                    >  1:3372029/1‑53 (MQ=255)                                                   gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                  >  1:374560/1‑71 (MQ=255)                                                   gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                  <  1:1356241/71‑1 (MQ=255)                                                    ccATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                     >  1:1770903/1‑67 (MQ=255)                                                       tCCTCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAtt                          <  1:5050246/59‑1 (MQ=255)                                                       tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCAtt                                             <  1:1651732/40‑1 (MQ=255)                                                       tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAt                           <  1:5245108/58‑1 (MQ=255)                                                       tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTATCTGTCCg              <  1:4469926/71‑1 (MQ=255)                                                       tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGt                 <  1:4249346/68‑1 (MQ=255)                                                        ccGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                         >  1:3383283/1‑59 (MQ=255)                                                         cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                        <  1:1549572/43‑1 (MQ=255)                                                         cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                        <  1:2241053/43‑1 (MQ=255)                                                         cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                        <  1:836306/43‑1 (MQ=255)                                                         cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg             >  1:602812/1‑70 (MQ=255)                                                         cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg             >  1:1237253/1‑70 (MQ=255)                                                         cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg             >  1:1152682/1‑70 (MQ=255)                                                          gtgcggcgTTCACCGCTTTTACTGCCGCAATGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGcg           <  1:140230/69‑2 (MQ=255)                                                          gtgcggcgTTCACCGCTTTTACTGCCGCAATGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGcg           <  1:2659374/69‑2 (MQ=255)                                                          gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                       <  1:3806820/43‑1 (MQ=255)                                                          gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                       <  1:5290970/43‑1 (MQ=255)                                                          gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                       <  1:2725174/43‑1 (MQ=255)                                                          gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGc            >  1:4318269/1‑70 (MQ=255)                                                          gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGc            >  1:367462/1‑70 (MQ=255)                                                           cgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCa                                      >  1:5461145/1‑43 (MQ=255)                                                              ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                       <  1:37973/39‑1 (MQ=255)                                                              ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                       <  1:4747398/39‑1 (MQ=255)                                                              ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg       <  1:2229390/71‑1 (MQ=255)                                                              ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg       <  1:3983882/71‑1 (MQ=255)                                                               gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg      <  1:47870/71‑1 (MQ=255)                                                                cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                     >  1:5543898/1‑39 (MQ=255)                                                                cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                     >  1:5091175/1‑39 (MQ=255)                                                                cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa     >  1:4124898/1‑71 (MQ=255)                                                                 gTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt    >  1:2398074/1‑71 (MQ=255)                                                                 gTTTACTGCTTACACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt    >  1:4688571/1‑71 (MQ=255)                                                                  tttACTGCTTTCACGGCATTAATGGCATTACGTTc                                       <  1:718600/35‑1 (MQ=255)                                                                  tttACTGCTTTCACGGCATTAATGGCATTACGTTc                                       <  1:4501103/35‑1 (MQ=255)                                                                  tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCa           <  1:1033904/63‑1 (MQ=255)                                                                   ttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAAc         <  1:2483797/64‑1 (MQ=255)                                                                    tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg  >  1:3354777/1‑70 (MQ=255)                                                                    tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg  >  1:1807330/1‑70 (MQ=255)                                                                    tACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCACCGGATTTGTACCTg                  >  1:360010/1‑54 (MQ=38)                                                                    |                                                                      TCTCGATCACTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCG  >  Exported/2093573‑2093709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑