BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,093,670	T→C	11.9%	E394E (GAA→GAG)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,093,670	0	T	C	11.9%	254.0 / 21.5	101	E394E (GAA→GAG)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand): ref base T (46/43); new base C (7/5); total (53/48)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 104 total aligned reads displayed.

GGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGC  >  Exported/2093602‑2093738

ggTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                                                    <  1:4699382/71‑1 (MQ=255)

ggTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                                                    <  1:1307244/71‑1 (MQ=255)

 gTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                                                    >  1:1051328/1‑70 (MQ=255)

 gTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                                                    <  1:109478/70‑1 (MQ=255)

  tGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                                                  <  1:4429569/71‑1 (MQ=255)

       aGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCAc                                                             >  1:3772774/1‑71 (MQ=255)

       aGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCAc                                                             >  1:3369357/1‑71 (MQ=255)

           gcggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGa                                                         >  1:3882536/1‑71 (MQ=255)

           gcggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGa                                                         >  1:2599924/1‑71 (MQ=255)

                 cATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGt                                                    <  1:1185664/70‑1 (MQ=255)

                  aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                                                    <  1:1292180/53‑1 (MQ=255)

                  aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                  >  1:5358735/1‑71 (MQ=255)

                   tCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                                                  >  1:759590/1‑54 (MQ=255)

                   tCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                                                  >  1:5364519/1‑54 (MQ=255)

                     gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAAt                                                                 >  1:3372029/1‑53 (MQ=255)

                     gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                               >  1:374560/1‑71 (MQ=255)

                     gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                               <  1:1356241/71‑1 (MQ=255)

                      ccATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                  >  1:1770903/1‑67 (MQ=255)

                         tCCTCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAtt                                                       <  1:5050246/59‑1 (MQ=255)

                         tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAt                                                        <  1:5245108/58‑1 (MQ=255)

                         tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTATCTGTCCg                                           <  1:4469926/71‑1 (MQ=255)

                         tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGt                                              <  1:4249346/68‑1 (MQ=255)

                          ccGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                      >  1:3383283/1‑59 (MQ=255)

                           cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                                                     <  1:1549572/43‑1 (MQ=255)

                           cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                                                     <  1:836306/43‑1 (MQ=255)

                           cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                                                     <  1:2241053/43‑1 (MQ=255)

                           cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                          >  1:1237253/1‑70 (MQ=255)

                           cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                          >  1:602812/1‑70 (MQ=255)

                           cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                          >  1:1152682/1‑70 (MQ=255)

                            gtgcggcgTTCACCGCTTTTACTGCCGCAATGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGcg                                        <  1:140230/69‑2 (MQ=255)

                            gtgcggcgTTCACCGCTTTTACTGCCGCAATGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGcg                                        <  1:2659374/69‑2 (MQ=255)

                            gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                                                    <  1:2725174/43‑1 (MQ=255)

                            gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                                                    <  1:5290970/43‑1 (MQ=255)

                            gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                                                    <  1:3806820/43‑1 (MQ=255)

                            gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGc                                         >  1:4318269/1‑70 (MQ=255)

                             cgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCa                                                                   >  1:5461145/1‑43 (MQ=255)

                                ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                                                    <  1:4747398/39‑1 (MQ=255)

                                ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                                                    <  1:37973/39‑1 (MQ=255)

                                ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                    <  1:2229390/71‑1 (MQ=255)

                                ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                    <  1:3983882/71‑1 (MQ=255)

                                  cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                                                  >  1:5543898/1‑39 (MQ=255)

                                  cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                                                  >  1:5091175/1‑39 (MQ=255)

                                  cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                                  >  1:4124898/1‑71 (MQ=255)

                                   gTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                 >  1:2398074/1‑71 (MQ=255)

                                   gTTTACTGCTTACACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                 >  1:4688571/1‑71 (MQ=255)

                                    tttACTGCTTTCACGGCATTAATGGCATTACGTTc                                                                    <  1:718600/35‑1 (MQ=255)

                                    tttACTGCTTTCACGGCATTAATGGCATTACGTTc                                                                    <  1:4501103/35‑1 (MQ=255)

                                    tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                        <  1:1033904/63‑1 (MQ=255)

                                     ttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAAc                                      <  1:2483797/64‑1 (MQ=255)

                                      tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                               >  1:1807330/1‑70 (MQ=255)

                                      tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                               >  1:3354777/1‑70 (MQ=255)

                                      tACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCACCGGATTTGTACCTg                                               >  1:360010/1‑54 (MQ=38)

                                       aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACggg                                                          >  1:2728630/1‑42 (MQ=255)

                                       aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAcc                                                 >  1:4997200/1‑51 (MQ=255)

                                       aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAcc                                                 >  1:384749/1‑51 (MQ=255)

                                       aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                               >  1:3965734/1‑69 (MQ=255)

                                       aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                               >  1:428430/1‑69 (MQ=255)

                                       aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGc                              <  1:1246911/70‑1 (MQ=255)

                                       aCTGCTTTCACGGCATTAAAGGCATTACGTTCAATGCACGGGATTTGTAcc                                                 >  1:224919/1‑51 (MQ=255)

                                       aCTGCTTTCACGGAATTAATGGCATTACGTTCAATGCACggg                                                          >  1:1392820/1‑42 (MQ=255)

                                         tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACgg                                                           <  1:327343/39‑1 (MQ=255)

                                           cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                          <  1:870489/70‑1 (MQ=255)

                                             ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                       >  1:3275880/1‑71 (MQ=255)

                                              tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                      >  1:5304007/1‑71 (MQ=255)

                                                 cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                        <  1:4757448/50‑1 (MQ=255)

                                                  ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                  <  1:4238672/71‑1 (MQ=255)

                                                  ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                  <  1:1316666/71‑1 (MQ=255)

                                                  ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                  <  1:4417386/71‑1 (MQ=255)

                                                    cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                >  1:1290078/1‑71 (MQ=255)

                                                     aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt               <  1:3518293/71‑1 (MQ=255)

                                                        aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt            >  1:62536/1‑71 (MQ=255)

                                                         aTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGaccg                   <  1:4700496/63‑2 (MQ=255)

                                                          tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                   <  1:2850388/62‑1 (MQ=255)

                                                          tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                   <  1:4082327/62‑1 (MQ=255)

                                                           ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt         >  1:72115/1‑71 (MQ=255)

                                                           ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt         >  1:5369122/1‑71 (MQ=255)

                                                           ggCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCt         >  1:2510150/1‑71 (MQ=255)

                                                            gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                            >  1:1661883/1‑51 (MQ=255)

                                                               ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                        <  1:4158560/36‑1 (MQ=255)

                                                               ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                <  1:3464394/60‑1 (MQ=255)

                                                               ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                <  1:1350301/60‑1 (MQ=255)

                                                               ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                <  1:1899907/60‑1 (MQ=255)

                                                               ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                <  1:5595479/60‑1 (MQ=255)

                                                               ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt            <  1:4430465/64‑1 (MQ=255)

                                                               ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAgg                <  1:5253238/60‑1 (MQ=255)

                                                               ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAt     <  1:2779624/71‑1 (MQ=255)

                                                                tACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                        <  1:3204794/35‑1 (MQ=255)

                                                                 aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                          >  1:2178079/1‑48 (MQ=255)

                                                                 aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTc                         >  1:121262/1‑49 (MQ=255)

                                                                 aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTc                         >  1:511074/1‑49 (MQ=255)

                                                                 aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                    >  1:4994782/1‑54 (MQ=255)

                                                                 aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg   >  1:114394/1‑71 (MQ=255)

                                                                 aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg   >  1:5653179/1‑71 (MQ=255)

                                                                 aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAgac                     >  1:5432081/1‑51 (MQ=255)

                                                                 aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGtt              >  1:605005/1‑60 (MQ=255)

                                                                 aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt         >  1:346/1‑65 (MQ=255)

                                                                 aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg   >  1:1677202/1‑69 (MQ=255)

                                                                  cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc  <  1:3410395/71‑1 (MQ=255)

                                                                  cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc  <  1:1106021/71‑1 (MQ=255)

                                                                   gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                          <  1:2960225/46‑1 (MQ=255)

GGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGC  >  Exported/2093602‑2093738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`