Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,093,673	A→G	13.3%	I393I (ATT→ATC)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,093,673	0	A	G	13.3%	219.8 / 22.2	90	I393I (ATT→ATC)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base A (46/32);  new base G (7/5);  total (53/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

TGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTT  >  Exported/2093604‑2093743                                                                      |                                                                       tGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                                                       <  1:4429569/71‑1 (MQ=255)   ccGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATg                                                                     >  1:2188227/1‑71 (MQ=255)      aGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCAc                                                                  >  1:3369357/1‑71 (MQ=255)      aGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCAc                                                                  >  1:3772774/1‑71 (MQ=255)          gcggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGa                                                              >  1:2599924/1‑71 (MQ=255)          gcggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGa                                                              >  1:3882536/1‑71 (MQ=255)                cATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGt                                                         <  1:1185664/70‑1 (MQ=255)                 aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                       >  1:5358735/1‑71 (MQ=255)                  tCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                                                       >  1:5364519/1‑54 (MQ=255)                  tCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                                                       >  1:759590/1‑54 (MQ=255)                    gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAAt                                                                      >  1:3372029/1‑53 (MQ=255)                    gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                                    <  1:1356241/71‑1 (MQ=255)                    gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                                    >  1:374560/1‑71 (MQ=255)                     ccATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                       >  1:1770903/1‑67 (MQ=255)                        tCCTCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAtt                                                            <  1:5050246/59‑1 (MQ=255)                        tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAt                                                             <  1:5245108/58‑1 (MQ=255)                        tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTATCTGTCCg                                                <  1:4469926/71‑1 (MQ=255)                        tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGt                                                   <  1:4249346/68‑1 (MQ=255)                         ccGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                           >  1:3383283/1‑59 (MQ=255)                          cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                               >  1:1152682/1‑70 (MQ=255)                          cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                               >  1:602812/1‑70 (MQ=255)                          cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                               >  1:1237253/1‑70 (MQ=255)                           gtgcggcgTTCACCGCTTTTACTGCCGCAATGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGcg                                             <  1:140230/69‑2 (MQ=255)                           gtgcggcgTTCACCGCTTTTACTGCCGCAATGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGcg                                             <  1:2659374/69‑2 (MQ=255)                           gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGc                                              >  1:4318269/1‑70 (MQ=255)                           gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGc                                              >  1:367462/1‑70 (MQ=255)                            cgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCa                                                                        >  1:5461145/1‑43 (MQ=255)                               ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                         <  1:3983882/71‑1 (MQ=255)                               ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                         <  1:2229390/71‑1 (MQ=255)                                gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                        <  1:47870/71‑1 (MQ=255)                                 cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                                                       >  1:5543898/1‑39 (MQ=255)                                 cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                                                       >  1:5091175/1‑39 (MQ=255)                                 cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                                       >  1:4124898/1‑71 (MQ=255)                                  gTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                      >  1:2398074/1‑71 (MQ=255)                                  gTTTACTGCTTACACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                      >  1:4688571/1‑71 (MQ=255)                                   tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                             <  1:1033904/63‑1 (MQ=255)                                    ttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAAc                                           <  1:2483797/64‑1 (MQ=255)                                     tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                    >  1:3354777/1‑70 (MQ=255)                                     tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                    >  1:1807330/1‑70 (MQ=255)                                     tACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCACCGGATTTGTACCTg                                                    >  1:360010/1‑54 (MQ=38)                                      aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACggg                                                               >  1:2728630/1‑42 (MQ=255)                                      aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAcc                                                      >  1:384749/1‑51 (MQ=255)                                      aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAcc                                                      >  1:4997200/1‑51 (MQ=255)                                      aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                    >  1:428430/1‑69 (MQ=255)                                      aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                    >  1:3965734/1‑69 (MQ=255)                                      aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGc                                   <  1:1246911/70‑1 (MQ=255)                                      aCTGCTTTCACGGCATTAAAGGCATTACGTTCAATGCACGGGATTTGTAcc                                                      >  1:224919/1‑51 (MQ=255)                                      aCTGCTTTCACGGAATTAATGGCATTACGTTCAATGCACggg                                                               >  1:1392820/1‑42 (MQ=255)                                        tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACgg                                                                <  1:327343/39‑1 (MQ=255)                                          cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                               <  1:870489/70‑1 (MQ=255)                                            ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                            >  1:3275880/1‑71 (MQ=255)                                             tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                           >  1:5304007/1‑71 (MQ=255)                                                cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                             <  1:4757448/50‑1 (MQ=255)                                                 ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                       <  1:1316666/71‑1 (MQ=255)                                                 ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                       <  1:4238672/71‑1 (MQ=255)                                                 ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                       <  1:4417386/71‑1 (MQ=255)                                                   cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                     >  1:1290078/1‑71 (MQ=255)                                                    aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                    <  1:3518293/71‑1 (MQ=255)                                                       aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                 >  1:62536/1‑71 (MQ=255)                                                        aTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGaccg                        <  1:4700496/63‑2 (MQ=255)                                                         tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                        <  1:4082327/62‑1 (MQ=255)                                                         tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                        <  1:2850388/62‑1 (MQ=255)                                                          ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt              >  1:5369122/1‑71 (MQ=255)                                                          ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt              >  1:72115/1‑71 (MQ=255)                                                          ggCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCt              >  1:2510150/1‑71 (MQ=255)                                                           gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                 >  1:1661883/1‑51 (MQ=255)                                                              ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                             <  1:4158560/36‑1 (MQ=255)                                                              ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                     <  1:5595479/60‑1 (MQ=255)                                                              ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                     <  1:3464394/60‑1 (MQ=255)                                                              ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                     <  1:1350301/60‑1 (MQ=255)                                                              ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                     <  1:1899907/60‑1 (MQ=255)                                                              ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                 <  1:4430465/64‑1 (MQ=255)                                                              ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAgg                     <  1:5253238/60‑1 (MQ=255)                                                              ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAt          <  1:2779624/71‑1 (MQ=255)                                                               tACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                             <  1:3204794/35‑1 (MQ=255)                                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                               >  1:2178079/1‑48 (MQ=255)                                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTc                              >  1:121262/1‑49 (MQ=255)                                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTc                              >  1:511074/1‑49 (MQ=255)                                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                         >  1:4994782/1‑54 (MQ=255)                                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg        >  1:5653179/1‑71 (MQ=255)                                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg        >  1:114394/1‑71 (MQ=255)                                                                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAgac                          >  1:5432081/1‑51 (MQ=255)                                                                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGtt                   >  1:605005/1‑60 (MQ=255)                                                                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt              >  1:346/1‑65 (MQ=255)                                                                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg        >  1:944373/1‑69 (MQ=255)                                                                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg        >  1:1677202/1‑69 (MQ=255)                                                                 cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc       <  1:3410395/71‑1 (MQ=255)                                                                 cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc       <  1:1106021/71‑1 (MQ=255)                                                                  gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                               <  1:2960225/46‑1 (MQ=255)                                                                     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                  >  1:4516193/1‑56 (MQ=255)                                                                     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                  >  1:4494426/1‑56 (MQ=255)                                                                     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                  >  1:3693864/1‑56 (MQ=255)                                                                     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt   >  1:3590660/1‑71 (MQ=255)                                                                     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt   >  1:3584056/1‑71 (MQ=255)                                                                     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt   >  1:1451747/1‑71 (MQ=255)                                                                     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt    <  1:4537438/70‑1 (MQ=255)                                                                      aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt  <  1:1529390/71‑1 (MQ=255)                                                                      |                                                                       TGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTT  >  Exported/2093604‑2093743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑