BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,093,700	A→G	13.7%	V384V (GTT→GTC)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,093,700	0	A	G	13.7%	267.7 / 29.1	109	V384V (GTT→GTC)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand): ref base A (49/45); new base G (12/3); total (61/48)
Fisher's exact test for biased strand distribution p-value = 5.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 113 total aligned reads displayed.

GGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCC  >  Exported/2093634‑2093766

ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                                                <  1:3983882/71‑1 (MQ=255)

ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                                                <  1:2229390/71‑1 (MQ=255)

 gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                               <  1:47870/71‑1 (MQ=255)

  cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                                                              >  1:4124898/1‑71 (MQ=255)

   gTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                             >  1:2398074/1‑71 (MQ=255)

   gTTTACTGCTTACACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                             >  1:4688571/1‑71 (MQ=255)

    tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                                                    <  1:1033904/63‑1 (MQ=255)

     ttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAAc                                                                  <  1:2483797/64‑1 (MQ=255)

      tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                           >  1:3354777/1‑70 (MQ=255)

      tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                           >  1:1807330/1‑70 (MQ=255)

       aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                           >  1:428430/1‑69 (MQ=255)

       aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                           >  1:3965734/1‑69 (MQ=255)

       aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGc                                                          <  1:1246911/70‑1 (MQ=255)

           cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                      <  1:870489/70‑1 (MQ=255)

             ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                                   >  1:3275880/1‑71 (MQ=255)

              tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                                                  >  1:5304007/1‑71 (MQ=255)

                 cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                                                    <  1:4757448/50‑1 (MQ=255)

                  ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                              <  1:1316666/71‑1 (MQ=255)

                  ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                              <  1:4417386/71‑1 (MQ=255)

                  ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                              <  1:4238672/71‑1 (MQ=255)

                    cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                            >  1:1290078/1‑71 (MQ=255)

                     aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                           <  1:3518293/71‑1 (MQ=255)

                        aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                        >  1:62536/1‑71 (MQ=255)

                         aTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGaccg                                               <  1:4700496/63‑2 (MQ=255)

                           ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                     >  1:5369122/1‑71 (MQ=255)

                           ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                     >  1:72115/1‑71 (MQ=255)

                           ggCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCt                                     >  1:2510150/1‑71 (MQ=255)

                            gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                        >  1:1661883/1‑51 (MQ=255)

                               ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                                                    <  1:4158560/36‑1 (MQ=255)

                               ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                            <  1:1899907/60‑1 (MQ=255)

                               ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                            <  1:3464394/60‑1 (MQ=255)

                               ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                            <  1:1350301/60‑1 (MQ=255)

                               ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                            <  1:5595479/60‑1 (MQ=255)

                               ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                        <  1:4430465/64‑1 (MQ=255)

                               ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAgg                                            <  1:5253238/60‑1 (MQ=255)

                               ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAt                                 <  1:2779624/71‑1 (MQ=255)

                                tACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                                                    <  1:3204794/35‑1 (MQ=255)

                                 aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                      >  1:2178079/1‑48 (MQ=255)

                                 aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTc                                                     >  1:511074/1‑49 (MQ=255)

                                 aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTc                                                     >  1:121262/1‑49 (MQ=255)

                                 aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                >  1:4994782/1‑54 (MQ=255)

                                 aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                               >  1:114394/1‑71 (MQ=255)

                                 aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                               >  1:5653179/1‑71 (MQ=255)

                                 aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAgac                                                 >  1:5432081/1‑51 (MQ=255)

                                  cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                              <  1:1106021/71‑1 (MQ=255)

                                  cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                              <  1:3410395/71‑1 (MQ=255)

                                   gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                      <  1:2960225/46‑1 (MQ=255)

                                      cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                         >  1:4516193/1‑56 (MQ=255)

                                      cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                         >  1:3693864/1‑56 (MQ=255)

                                      cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                          >  1:1451747/1‑71 (MQ=255)

                                      cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                          >  1:3584056/1‑71 (MQ=255)

                                      cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                          >  1:3590660/1‑71 (MQ=255)

                                      cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt                           <  1:4537438/70‑1 (MQ=255)

                                       aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                         <  1:1529390/71‑1 (MQ=255)

                                         tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                           <  1:226556/51‑1 (MQ=255)

                                         tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                           <  1:4015058/51‑1 (MQ=255)

                                         tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                           <  1:4681619/51‑1 (MQ=255)

                                          gCACGGGATTTGTACCTGTCCGGCAACCGGATCGc                                                          <  1:1708365/35‑1 (MQ=255)

                                          gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                        <  1:236864/53‑1 (MQ=255)

                                           cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                     >  1:1744041/1‑71 (MQ=255)

                                            aCGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGc                                      >  1:216264/1‑53 (MQ=255)

                                             cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                   <  1:381/71‑1 (MQ=255)

                                             cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                   <  1:5343954/71‑1 (MQ=255)

                                              gggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                       >  1:1219149/1‑66 (MQ=255)

                                                 aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                         <  1:686516/45‑1 (MQ=255)

                                                 aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                         >  1:2327529/1‑45 (MQ=255)

                                                  tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCGAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg              >  1:1978175/1‑71 (MQ=255)

                                                  tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                        <  1:3246839/45‑1 (MQ=255)

                                                  tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                        <  1:3523781/45‑1 (MQ=255)

                                                  tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                   <  1:5240538/50‑1 (MQ=255)

                                                  tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                   <  1:4156908/50‑1 (MQ=255)

                                                  tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                   <  1:20459/50‑1 (MQ=255)

                                                   ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg              >  1:5319644/1‑70 (MQ=255)

                                                    tGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCa                                  >  1:3967197/1‑49 (MQ=255)

                                                    tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCAt                                 >  1:2946154/1‑50 (MQ=255)

                                                     gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTtgtg                                       >  1:5605557/1‑43 (MQ=255)

                                                     gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa            >  1:2198475/1‑70 (MQ=12)

                                                     gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa            >  1:2405313/1‑70 (MQ=12)

                                                     gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa            <  1:1369682/70‑1 (MQ=255)

                                                        ccTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc        >  1:885311/1‑71 (MQ=255)

                                                         cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGTGATTTCCGCCGCATTGCAt           <  1:774486/67‑1 (MQ=255)

                                                         cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                >  1:1351605/1‑62 (MQ=255)

                                                         cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt           <  1:2939965/67‑1 (MQ=255)

                                                           gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAc         >  1:810687/1‑67 (MQ=255)

                                                            tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTgcg    >  1:2494244/1‑71 (MQ=255)

                                                            tCCGGCAACCGGATCGCAGGTCAGGCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgc     >  1:2755031/1‑70 (MQ=255)

                                                            tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                         >  1:4332631/1‑50 (MQ=255)

                                                            tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg              <  1:5115985/61‑1 (MQ=255)

                                                            tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg              <  1:1450476/61‑1 (MQ=255)

                                                            tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg              <  1:1704363/61‑1 (MQ=255)

                                                            tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg              <  1:3523794/61‑1 (MQ=255)

                                                            tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt           <  1:1091913/64‑1 (MQ=255)

                                                            tCCGCCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt           <  1:5143837/64‑1 (MQ=255)

                                                             ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                     >  1:3493960/1‑53 (MQ=255)

                                                             ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                     >  1:2417862/1‑53 (MQ=255)

                                                             ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                     >  1:3931271/1‑53 (MQ=255)

                                                             ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                     >  1:5033261/1‑53 (MQ=255)

                                                             ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                     >  1:5102481/1‑53 (MQ=255)

                                                              cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa            >  1:4030355/1‑61 (MQ=255)

                                                              cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc  >  1:1777827/1‑71 (MQ=255)

GGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCC  >  Exported/2093634‑2093766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`