BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,093,712	G→C	16.0%	T380T (ACC→ACG)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,093,712	0	G	C	16.0%	258.6 / 35.7	106	T380T (ACC→ACG)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand): ref base G (43/46); new base C (13/4); total (56/50)
Fisher's exact test for biased strand distribution p-value = 3.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 107 total aligned reads displayed.

CTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAG  >  Exported/2093645‑2093780

cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                                    <  1:870489/70‑1 (MQ=255)

   tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                                                                >  1:5304007/1‑71 (MQ=255)

       ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                            <  1:4417386/71‑1 (MQ=255)

       ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                            <  1:4238672/71‑1 (MQ=255)

       ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                            <  1:1316666/71‑1 (MQ=255)

         cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                          >  1:1290078/1‑71 (MQ=255)

          aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                         <  1:3518293/71‑1 (MQ=255)

             aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                      >  1:62536/1‑71 (MQ=255)

              aTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGaccg                                                             <  1:4700496/63‑2 (MQ=255)

               tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                                                             <  1:4082327/62‑1 (MQ=255)

               tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                                                             <  1:2850388/62‑1 (MQ=255)

                ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                                   >  1:72115/1‑71 (MQ=255)

                ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                                   >  1:5369122/1‑71 (MQ=255)

                ggCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCt                                                   >  1:2510150/1‑71 (MQ=255)

                 gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                                      >  1:1661883/1‑51 (MQ=255)

                    ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                          <  1:1350301/60‑1 (MQ=255)

                    ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                          <  1:5595479/60‑1 (MQ=255)

                    ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                          <  1:1899907/60‑1 (MQ=255)

                    ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                          <  1:3464394/60‑1 (MQ=255)

                    ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                      <  1:4430465/64‑1 (MQ=255)

                    ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAgg                                                          <  1:5253238/60‑1 (MQ=255)

                    ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAt                                               <  1:2779624/71‑1 (MQ=255)

                      aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                                    >  1:2178079/1‑48 (MQ=255)

                      aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTc                                                                   >  1:121262/1‑49 (MQ=255)

                      aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTc                                                                   >  1:511074/1‑49 (MQ=255)

                      aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                              >  1:4994782/1‑54 (MQ=255)

                      aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                             >  1:5653179/1‑71 (MQ=255)

                      aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                             >  1:114394/1‑71 (MQ=255)

                      aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAgac                                                               >  1:5432081/1‑51 (MQ=255)

                      aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGtt                                                        >  1:605005/1‑60 (MQ=255)

                      aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                                   >  1:346/1‑65 (MQ=255)

                       cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                            <  1:1106021/71‑1 (MQ=255)

                       cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                            <  1:3410395/71‑1 (MQ=255)

                        gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                                    <  1:2960225/46‑1 (MQ=255)

                           cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                       >  1:4516193/1‑56 (MQ=255)

                           cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                       >  1:3693864/1‑56 (MQ=255)

                           cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                       >  1:4494426/1‑56 (MQ=255)

                           cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                        >  1:3584056/1‑71 (MQ=255)

                           cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                        >  1:3590660/1‑71 (MQ=255)

                           cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                        >  1:1451747/1‑71 (MQ=255)

                           cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt                                         <  1:4537438/70‑1 (MQ=255)

                            aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                       <  1:1529390/71‑1 (MQ=255)

                              tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                         <  1:4015058/51‑1 (MQ=255)

                              tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                         <  1:226556/51‑1 (MQ=255)

                                cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                   >  1:1744041/1‑71 (MQ=255)

                                 aCGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGc                                                    >  1:216264/1‑53 (MQ=255)

                                  cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                                 <  1:381/71‑1 (MQ=255)

                                   gggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                     >  1:1219149/1‑66 (MQ=255)

                                     gATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                      <  1:4799612/47‑1 (MQ=255)

                                      aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                       <  1:686516/45‑1 (MQ=255)

                                      aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                       >  1:2327529/1‑45 (MQ=255)

                                       tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCGAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                            >  1:1978175/1‑71 (MQ=255)

                                       tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                      <  1:3523781/45‑1 (MQ=255)

                                       tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                      <  1:3246839/45‑1 (MQ=255)

                                       tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                                 <  1:20459/50‑1 (MQ=255)

                                       tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                                 <  1:5240538/50‑1 (MQ=255)

                                       tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                                 <  1:4156908/50‑1 (MQ=255)

                                        ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                            >  1:5319644/1‑70 (MQ=255)

                                         tGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCa                                                >  1:3967197/1‑49 (MQ=255)

                                         tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCAt                                               >  1:2946154/1‑50 (MQ=255)

                                          gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTtgtg                                                     >  1:5605557/1‑43 (MQ=255)

                                          gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                          >  1:2198475/1‑70 (MQ=12)

                                          gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                          >  1:2405313/1‑70 (MQ=12)

                                          gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                          <  1:1369682/70‑1 (MQ=255)

                                             ccTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc                      >  1:885311/1‑71 (MQ=255)

                                              cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGTGATTTCCGCCGCATTGCAt                         <  1:774486/67‑1 (MQ=255)

                                              cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                              >  1:3664762/1‑62 (MQ=255)

                                              cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                              >  1:1351605/1‑62 (MQ=255)

                                              cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                         <  1:2939965/67‑1 (MQ=255)

                                                gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAc                       >  1:810687/1‑67 (MQ=255)

                                                 tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTgcg                  >  1:2494244/1‑71 (MQ=255)

                                                 tCCGGCAACCGGATCGCAGGTCAGGCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgc                   >  1:2755031/1‑70 (MQ=255)

                                                 tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                       >  1:4332631/1‑50 (MQ=255)

                                                 tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                       >  1:206288/1‑50 (MQ=255)

                                                 tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                       >  1:2369391/1‑50 (MQ=255)

                                                 tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                            <  1:3523794/61‑1 (MQ=255)

                                                 tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                            <  1:1450476/61‑1 (MQ=255)

                                                 tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                            <  1:1704363/61‑1 (MQ=255)

                                                 tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                            <  1:5115985/61‑1 (MQ=255)

                                                 tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                         <  1:1091913/64‑1 (MQ=255)

                                                 tCCGCCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                         <  1:5143837/64‑1 (MQ=255)

                                                  ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                   >  1:5102481/1‑53 (MQ=255)

                                                  ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                   >  1:5033261/1‑53 (MQ=255)

                                                  ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                   >  1:3931271/1‑53 (MQ=255)

                                                  ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                   >  1:3493960/1‑53 (MQ=255)

                                                  ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                   >  1:2417862/1‑53 (MQ=255)

                                                   cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                       >  1:1296817/1‑48 (MQ=255)

                                                   cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                          >  1:4030355/1‑61 (MQ=255)

                                                   cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                >  1:5285012/1‑71 (MQ=255)

                                                   cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                >  1:1777827/1‑71 (MQ=255)

                                                     gcgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCgg              >  1:3939444/4‑71 (MQ=9)

                                                     gcgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCgg              <  1:5587325/68‑1 (MQ=9)

                                                           ggATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTg                    >  1:3450218/1‑59 (MQ=255)

                                                             aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgccgc      <  1:3127635/71‑1 (MQ=255)

                                                                gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                     >  1:2162419/1‑37 (MQ=255)

                                                                gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg              <  1:4934977/60‑1 (MQ=255)

                                                                gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg              <  1:1952331/60‑1 (MQ=255)

                                                                gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg              <  1:2574925/60‑1 (MQ=255)

                                                                gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCa   <  1:3822503/71‑1 (MQ=255)

                                                                 cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag  <  1:3440753/71‑1 (MQ=255)

CTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAG  >  Exported/2093645‑2093780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`