Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,093,751	A→G	11.1%	N367N (AAT→AAC) ‡	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,093,751	0	A	G	11.1%	165.9 / 9.9	63	N367N (AAT→AAC) ‡	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base A (28/28);  new base G (5/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

TTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGG  >  Exported/2093684‑2093815                                                                    |                                                                 tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCGAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                               >  1:1978175/1‑71 (MQ=255)  ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                               >  1:5319644/1‑70 (MQ=255)    gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                                                             >  1:2405313/1‑70 (MQ=12)    gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                                                             >  1:2198475/1‑70 (MQ=12)    gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                                                             <  1:1369682/70‑1 (MQ=255)       ccTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc                                                         >  1:885311/1‑71 (MQ=255)        cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGTGATTTCCGCCGCATTGCAt                                                            <  1:774486/67‑1 (MQ=255)        cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                                                 >  1:1351605/1‑62 (MQ=255)        cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                                                 >  1:3664762/1‑62 (MQ=255)        cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                                            <  1:2939965/67‑1 (MQ=255)          gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAc                                                          >  1:810687/1‑67 (MQ=255)           tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTgcg                                                     >  1:2494244/1‑71 (MQ=255)           tCCGGCAACCGGATCGCAGGTCAGGCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgc                                                      >  1:2755031/1‑70 (MQ=255)           tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                               <  1:3523794/61‑1 (MQ=255)           tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                               <  1:5115985/61‑1 (MQ=255)           tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                               <  1:1450476/61‑1 (MQ=255)           tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                               <  1:1704363/61‑1 (MQ=255)           tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                                            <  1:1091913/64‑1 (MQ=255)           tCCGCCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                                            <  1:5143837/64‑1 (MQ=255)             cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                                                             >  1:4030355/1‑61 (MQ=255)             cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                                                   >  1:1777827/1‑71 (MQ=255)             cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                                                   >  1:5285012/1‑71 (MQ=255)               gcgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCgg                                                 <  1:5587325/68‑1 (MQ=9)               gcgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCgg                                                 >  1:3939444/4‑71 (MQ=9)                     ggATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTg                                                       >  1:3450218/1‑59 (MQ=255)                       aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgccgc                                         <  1:3127635/71‑1 (MQ=255)                          gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                                                 <  1:1952331/60‑1 (MQ=255)                          gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                                                 <  1:2574925/60‑1 (MQ=255)                          gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                                                 <  1:4934977/60‑1 (MQ=255)                          gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                                                 <  1:3908258/60‑1 (MQ=255)                          gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCa                                      <  1:3822503/71‑1 (MQ=255)                           cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag                                     <  1:3440753/71‑1 (MQ=255)                               tCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt                                 <  1:3609381/71‑1 (MQ=255)                               gcAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                               <  1:5623993/40‑1 (MQ=255)                                 aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt                                <  1:577449/70‑1 (MQ=255)                                  gCCCAAGGTTATGCTCCATCGCGATTTCCGCCGGATAGCATAc                                                          >  1:322804/1‑43 (MQ=38)                                     cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGt                            >  1:556013/1‑70 (MQ=255)                                      aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa                          <  1:1068627/71‑1 (MQ=255)                                      aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa                          <  1:1061863/71‑1 (MQ=255)                                       aGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTaa                         <  1:3242207/71‑1 (MQ=255)                                        ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                             >  1:80479/1‑66 (MQ=255)                                        ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                             >  1:2795890/1‑66 (MQ=255)                                        ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                             >  1:1757215/1‑66 (MQ=255)                                        ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                             >  1:2639012/1‑66 (MQ=255)                                         gTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                       <  1:829348/71‑1 (MQ=255)                                         gTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                       <  1:4757352/71‑1 (MQ=255)                                                  cATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccg              >  1:5469700/1‑71 (MQ=255)                                                   aTCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc             >  1:3856781/1‑71 (MQ=255)                                                    tCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc             >  1:2843138/1‑70 (MQ=255)                                                     cgcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagta                                   >  1:4412515/1‑47 (MQ=255)                                                      gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGtt                           >  1:682396/1‑54 (MQ=255)                                                      gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                       >  1:1375060/1‑58 (MQ=255)                                                      gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                       >  1:2489800/1‑58 (MQ=255)                                                      gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                       >  1:5362224/1‑58 (MQ=255)                                                      gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAt          >  1:849638/1‑71 (MQ=255)                                                        gATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAtt         >  1:4173286/1‑70 (MQ=255)                                                        gATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTg        <  1:4048256/71‑1 (MQ=255)                                                         aTTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTc                               <  1:1469940/47‑1 (MQ=255)                                                          tttCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc             <  1:2904729/64‑1 (MQ=255)                                                             ccgccgCGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGagc     <  1:4323993/69‑3 (MQ=255)                                                             ccgccgCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACa    <  1:161852/70‑1 (MQ=255)                                                              cgccgcGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGAGCacg  >  1:4570746/1‑69 (MQ=255)                                                                   cATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCa           >  1:2495466/1‑57 (MQ=255)                                                                    |                                                                 TTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGG  >  Exported/2093684‑2093815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑