| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,093,955 | A→G | 15.8% | Y299Y (TAT→TAC) | tdcG ← | L‑serine dehydratase 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,093,955 | 0 | A | G | 15.8% | 218.3 / 30.0 | 95 | Y299Y (TAT→TAC) | tdcG | L‑serine dehydratase 3 |
| Reads supporting (aligned to +/- strand): ref base A (38/42); new base G (11/4); total (49/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.18e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 106 total aligned reads displayed.
TAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTG > Exported/2093888‑2094025 | tAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGtaat > 1:5227266/1‑69 (MQ=255)tAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGtaat > 1:3513614/1‑69 (MQ=255) cccGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAg < 1:5310707/71‑1 (MQ=255) ggCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGt < 1:4442776/67‑1 (MQ=255) gCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGc < 1:2753528/71‑1 (MQ=255) cAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGc < 1:868925/60‑1 (MQ=255) cAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGc < 1:2698739/60‑1 (MQ=255) cAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCg < 1:4122573/71‑1 (MQ=255) aGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCgg < 1:4537720/71‑1 (MQ=255) gAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCATTACTGCCGGa < 1:117037/71‑1 (MQ=255) gAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCgg > 1:624616/1‑70 (MQ=255) aaaTAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaat < 1:427164/71‑1 (MQ=255) aaaTAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaa > 1:1877559/1‑70 (MQ=255) aGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGtaa > 1:3384463/1‑47 (MQ=255) aGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGtaa > 1:670408/1‑47 (MQ=255) aGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGt < 1:4436167/56‑1 (MQ=255) gCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATga > 1:3436402/1‑70 (MQ=255) gCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATg < 1:3251234/70‑1 (MQ=255) gCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATg < 1:1789480/70‑1 (MQ=255) aaTTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaat < 1:3958981/60‑1 (MQ=255) aTTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgcacgc < 1:3536218/71‑1 (MQ=255) aTTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgcacgc < 1:3938239/71‑1 (MQ=255) aTTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgcacg > 1:2563832/1‑70 (MQ=255) tGACCGCTCGTTTACCGGACGACGGAACTTATCGtaat > 1:4542486/1‑38 (MQ=255) tGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTa > 1:2855360/1‑47 (MQ=255) tGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCgcacgcac > 1:4523193/1‑71 (MQ=255) gACCGCTCGTTTACCGGACGACGGAACTTATCGtaa > 1:820901/1‑36 (MQ=255) gACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCcgcacgc < 1:2546472/68‑1 (MQ=255) ccGCTCGTTTACCGGACGACGGAACTTATCGTAATATGCCAGTACTGCCGGAATAATGCCGCACGCACCGt < 1:1435165/71‑1 (MQ=255) ccGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGt < 1:2532291/71‑1 (MQ=255) ccGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGt < 1:5650663/71‑1 (MQ=255) ccGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGt < 1:3285351/71‑1 (MQ=255) cGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGt > 1:2341850/1‑70 (MQ=255) gCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCa > 1:3419113/1‑39 (MQ=255) gCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCa > 1:563282/1‑39 (MQ=255) gCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAg < 1:5287495/40‑1 (MQ=255) gCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAg < 1:1302585/40‑1 (MQ=255) gCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAg < 1:3204843/40‑1 (MQ=255) gCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAg < 1:2404873/40‑1 (MQ=255) gCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAg < 1:243809/40‑1 (MQ=255) gCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGt > 1:221553/1‑69 (MQ=255) cTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaat < 1:3132883/51‑1 (MQ=255) tCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTa > 1:4310112/1‑69 (MQ=255) tCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTa > 1:5664956/1‑69 (MQ=255) tCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGt > 1:4883251/1‑71 (MQ=255) tCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGt > 1:347994/1‑71 (MQ=255) gTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGt < 1:2980555/69‑1 (MQ=255) tACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCg > 1:2082869/1‑68 (MQ=255) cGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCggt < 1:1635642/40‑2 (MQ=255) cGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaat < 1:2474628/42‑1 (MQ=255) cGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaat < 1:3184121/42‑1 (MQ=255) ggACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGt < 1:351582/71‑1 (MQ=255) ggACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGt > 1:1516816/1‑71 (MQ=255) ggACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGt > 1:936761/1‑71 (MQ=255) ggACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGt > 1:815046/1‑71 (MQ=255) gacgacGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGtt < 1:3766914/71‑1 (MQ=255) gacgacGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGt > 1:1967858/1‑70 (MQ=255) acgacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTa < 1:4912529/71‑1 (MQ=255) gacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTc > 1:376702/1‑59 (MQ=255) gacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTc > 1:4076938/1‑59 (MQ=255) gacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAcc < 1:5573537/71‑1 (MQ=255) gacgGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAcc < 1:330598/71‑1 (MQ=255) ataaaCTTGTCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGgcgc < 1:4072408/59‑1 (MQ=255) taaaCTTGTCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGgcgc > 1:2086508/3‑61 (MQ=255) taaaCTTGTCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCAc > 1:5127424/3‑67 (MQ=255) ggAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcg > 1:164002/1‑71 (MQ=255) aaCTTGTCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAg < 1:2729027/52‑1 (MQ=25) aaCTTGTCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAg < 1:753465/52‑1 (MQ=25) aaCTTATCGTAATAAGCCAGTACTGCCGGAATAATg < 1:4587790/36‑1 (MQ=255) aCTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACgcgc > 1:4843/1‑71 (MQ=255) aCTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACgcgc > 1:3708721/1‑71 (MQ=255) cTTGTCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTc > 1:1343018/1‑52 (MQ=25) cTTGTCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGATAgac > 1:1826969/1‑50 (MQ=16) cTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAg < 1:4392894/50‑1 (MQ=255) cTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAg < 1:1925721/50‑1 (MQ=255) ttgtCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCAc < 1:3702030/59‑1 (MQ=255) ttgtCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGcgacc > 1:2066948/4‑71 (MQ=255) ttgtCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGcgacc > 1:5468454/4‑71 (MQ=255) ttATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTc < 1:3882923/51‑1 (MQ=255) ttATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTc < 1:1120625/51‑1 (MQ=255) tATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTa > 1:136755/1‑47 (MQ=255) tATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTa > 1:1510676/1‑47 (MQ=255) tATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTa > 1:5032431/1‑47 (MQ=255) tATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTa > 1:125019/1‑47 (MQ=255) tATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcgc > 1:217407/1‑66 (MQ=255) tATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcgc > 1:3890246/1‑66 (MQ=255) tATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcgc > 1:5502257/1‑66 (MQ=255) gtCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCa > 1:3176502/2‑62 (MQ=255) gtCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCa > 1:3169996/2‑62 (MQ=255) aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCgg > 1:3465369/1‑51 (MQ=255) aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTa > 1:4688323/1‑59 (MQ=255) aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc > 1:2676583/1‑71 (MQ=255) aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc > 1:3695590/1‑71 (MQ=255) aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc > 1:4637198/1‑71 (MQ=255) aTCGTAATAAGACAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTa > 1:168245/1‑59 (MQ=255) tCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTcaccac > 1:1527158/1‑62 (MQ=255) tCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTcaccac > 1:3411282/1‑62 (MQ=255) gtagtaCGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAg > 1:806680/1‑71 (MQ=255) ataaTCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGt < 1:790571/42‑1 (MQ=255) ataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTg > 1:4459363/1‑71 (MQ=255) | TAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTG > Exported/2093888‑2094025 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |