Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,094,024	T→A	13.1%	A276A (GCA→GCT)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,094,024	0	T	A	13.1%	199.0 / 20.2	76	A276A (GCA→GCT)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base T (34/32);  new base A (7/3);  total (41/35)
Fisher's exact test for biased strand distribution p-value = 3.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

ATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAG  >  Exported/2093955‑2094093                                                                      |                                                                      ataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTg                                                                      >  1:4459363/1‑71 (MQ=255)    aGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCg                                                                    <  1:659736/70‑1 (MQ=255)    aGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGt                                                                   >  1:2956915/1‑71 (MQ=255)     gCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCg                                                                    <  1:3037432/69‑1 (MQ=255)     gCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtt                                                                  <  1:5166382/71‑1 (MQ=255)     gCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtt                                                                  <  1:465832/71‑1 (MQ=255)      ccAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGttc                                                                 >  1:2181308/1‑70 (MQ=255)      ccAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGttc                                                                 >  1:2620699/1‑70 (MQ=255)      ccAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGttc                                                                 <  1:2552742/71‑2 (MQ=255)       cAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtttt                                                                >  1:1294979/1‑71 (MQ=255)       cAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtttt                                                                <  1:182662/71‑1 (MQ=255)       cAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtttt                                                                <  1:2560447/71‑1 (MQ=255)        aGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttc                                                               >  1:58555/1‑71 (MQ=255)        aGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttc                                                               >  1:3581412/1‑71 (MQ=255)          tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                             <  1:4390054/71‑1 (MQ=255)          tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                             <  1:3112079/71‑1 (MQ=255)            cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                             >  1:1208784/1‑69 (MQ=255)              gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtt                                                                  <  1:1173057/62‑1 (MQ=255)              gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCAc                                                          >  1:3455443/1‑70 (MQ=255)                cGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTaa                                                       <  1:384785/71‑1 (MQ=255)                 ggAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAAc                                                      >  1:3191954/1‑71 (MQ=255)                  ggataatCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGtt                                                                  <  1:5387372/56‑1 (MQ=255)                  gAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTaa                                                       >  1:4677484/1‑69 (MQ=255)                    ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                                   >  1:4308065/1‑71 (MQ=255)                    ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                                   >  1:5546509/1‑71 (MQ=255)                      aatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct                                                              <  1:2004588/58‑1 (MQ=255)                      aatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct                                                              <  1:604785/58‑1 (MQ=255)                      aatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct                                                              <  1:4361282/58‑1 (MQ=255)                        tcccGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgt                                               >  1:1552554/3‑70 (MQ=255)                           cgcacgcaCCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCcgcgcg                                            >  1:1346859/1‑71 (MQ=255)                            gcacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                            >  1:3312505/1‑70 (MQ=255)                            gcacgcacCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca                                            <  1:1044766/70‑2 (MQ=255)                            gcacgcacCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca                                            >  1:2334490/1‑69 (MQ=255)                             cacgcacCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgcaaa                                          >  1:620203/1‑68 (MQ=255)                               cgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa                                        >  1:5409053/1‑71 (MQ=255)                               cgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa                                        >  1:2567839/1‑71 (MQ=255)                                    cGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTg                                   <  1:2775499/71‑1 (MQ=255)                                    cGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAGTGCAAACATGt                                     >  1:5522383/1‑69 (MQ=255)                                     gTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAgc                                               >  1:2495112/1‑58 (MQ=255)                                     gTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                            <  1:5421752/61‑1 (MQ=255)                                     gTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGa                                  >  1:925203/1‑71 (MQ=255)                                     gTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGa                                  >  1:3041413/1‑71 (MQ=255)                                      ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                 >  1:5399221/1‑71 (MQ=255)                                      ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                 >  1:466892/1‑71 (MQ=255)                                       tAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGa                                  <  1:3601001/69‑1 (MQ=255)                                        aGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAGTGCAAACATGTTGATc                                >  1:3908176/1‑70 (MQ=255)                                          tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                             <  1:5136975/39‑1 (MQ=255)                                            ggTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTc                           >  1:2580445/1‑71 (MQ=255)                                            ggTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTc                           >  1:749459/1‑71 (MQ=255)                                                ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt                        >  1:2698284/1‑70 (MQ=255)                                                  gTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGa                         >  1:40654/1‑67 (MQ=255)                                                   ttACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa                    <  1:977343/71‑1 (MQ=255)                                                     aCCATGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATcc                               >  1:3007816/1‑58 (MQ=255)                                                     aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt                  >  1:520638/1‑71 (MQ=255)                                                     aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt                  >  1:4415825/1‑71 (MQ=255)                                                     aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt                  >  1:4967812/1‑71 (MQ=255)                                                     aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt                  >  1:2448947/1‑71 (MQ=255)                                                     aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt                  >  1:3248202/1‑71 (MQ=255)                                                      ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt                  <  1:3737987/70‑1 (MQ=255)                                                      ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt                  <  1:2656370/70‑1 (MQ=255)                                                       cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                 <  1:5581510/54‑1 (MQ=255)                                                       cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                 <  1:5061826/54‑1 (MQ=255)                                                       cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCa                >  1:2046177/1‑71 (MQ=255)                                                       cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCa                <  1:72116/71‑1 (MQ=255)                                                        aCGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCAt               >  1:2703277/1‑71 (MQ=255)                                                         cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGa                                  >  1:5388240/1‑51 (MQ=255)                                                            gcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGa           <  1:5030121/71‑1 (MQ=255)                                                             cccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATg                       <  1:4502263/58‑1 (MQ=255)                                                              ccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGa                      <  1:994867/58‑1 (MQ=255)                                                                 ccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                 <  1:1788105/44‑1 (MQ=255)                                                                 ccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGtt      <  1:2851214/71‑1 (MQ=255)                                                                  cAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTa     <  1:2731332/71‑1 (MQ=255)                                                                   aGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTa     <  1:2861085/70‑1 (MQ=255)                                                                     cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCa                <  1:4934026/57‑1 (MQ=255)                                                                     cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTagag  <  1:4755424/71‑1 (MQ=255)                                                                     cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTa     >  1:3741713/1‑68 (MQ=255)                                                                      tGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTaga   <  1:4431783/69‑1 (MQ=255)                                                                      tGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTaga   <  1:4081849/69‑1 (MQ=255)                                                                      tGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTaga   <  1:3252178/69‑1 (MQ=255)                                                                      |                                                                      ATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAG  >  Exported/2093955‑2094093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑