Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,096,609 G→C 6.7% G110G (GGC→GGG garK ← glycerate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,096,6090GC 6.7% 223.2 / 4.7 74G110G (GGC→GGGgarKglycerate kinase I
Reads supporting (aligned to +/- strand):  ref base G (37/32);  new base C (2/3);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

TGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTT  >  Exported/2096540‑2096679
                                                                     |                                                                      
tGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGc                                                                       <  1:522963/71‑1 (MQ=255)
tGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGc                                                                       <  1:4657247/71‑1 (MQ=255)
 ggATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGc                                                                       <  1:979523/70‑1 (MQ=255)
 ggATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGc                                                                       >  1:2143747/1‑70 (MQ=255)
  gATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGcc                                                                      <  1:5144728/70‑1 (MQ=255)
    tCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATc                                                                   >  1:146437/1‑71 (MQ=255)
          ttttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGcc                                                                >  1:2997059/1‑68 (MQ=255)
           tttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCg                                                               >  1:3010229/1‑68 (MQ=255)
           tttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGga                                                             <  1:5280595/70‑1 (MQ=255)
            ttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTg                                                                        >  1:1095900/1‑58 (MQ=255)
            ttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCg                                                               >  1:2439995/1‑67 (MQ=255)
             tCCGCAGGTACCTGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGgaga                                                           <  1:1582598/70‑1 (MQ=255)
              ccGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATcc                                                        >  1:787884/1‑71 (MQ=255)
               cGCATGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATc                                                         <  1:3388872/70‑1 (MQ=255)
                gCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccc                                                       >  1:4005276/1‑71 (MQ=255)
                     tACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc                                                  <  1:602647/71‑1 (MQ=255)
                       ccAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGcc                                                                      <  1:814367/49‑1 (MQ=255)
                        cAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTgg                                               <  1:3979976/71‑1 (MQ=255)
                         aGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGcc                                                                      <  1:4865336/47‑1 (MQ=255)
                           cTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATcc                                                        >  1:1091799/1‑59 (MQ=255)
                           cTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATc                                                         >  1:1253572/1‑58 (MQ=255)
                            tCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCaa                                                   >  1:2957591/1‑63 (MQ=255)
                              cAGCCCACTGGCGGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCa                                         <  1:2768444/71‑1 (MQ=255)
                                  ccACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACttt                                     <  1:506739/71‑1 (MQ=255)
                                    aCTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGgaga                                                           <  1:755891/47‑1 (MQ=255)
                                    aCTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCa                                                    <  1:2198443/54‑1 (MQ=255)
                                     cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc                                                     <  1:1745855/52‑1 (MQ=255)
                                     cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc                                                     <  1:4667717/52‑1 (MQ=255)
                                     cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtctc                                  >  1:3926480/1‑71 (MQ=255)
                                     cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCCGAGATccccc                                                     <  1:179563/52‑1 (MQ=255)
                                      tGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCg                                 >  1:3593843/1‑71 (MQ=255)
                                      tGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCg                                 >  1:4799295/1‑71 (MQ=255)
                                       ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCaa                                                   >  1:4515923/1‑52 (MQ=255)
                                       ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCaa                                                   >  1:3957323/1‑52 (MQ=255)
                                       ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc                                                  >  1:2219952/1‑53 (MQ=255)
                                       ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGc                                >  1:2411976/1‑71 (MQ=255)
                                        gctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc                               >  1:786951/4‑71 (MQ=255)
                                        gctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc                               >  1:697326/4‑71 (MQ=255)
                                         ctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc                               <  1:5535532/68‑1 (MQ=255)
                                         ccgccgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTgg                                               <  1:395542/54‑1 (MQ=255)
                                         ccgccgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGccc                              <  1:3151299/71‑1 (MQ=255)
                                            ccgccATTTCAATAAACGCGGTTTTGCCATCGCCg                                                               <  1:4194555/35‑1 (MQ=255)
                                            ccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGCCCa                             <  1:526871/69‑1 (MQ=255)
                                             cgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc                                                     <  1:3167975/44‑1 (MQ=255)
                                             cgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc                                                     <  1:5388770/44‑1 (MQ=255)
                                             cgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGCCCATCg                          <  1:5037709/71‑1 (MQ=255)
                                              gccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATc                                                         >  1:605773/1‑39 (MQ=255)
                                              gccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc                                                  >  1:5606833/1‑46 (MQ=255)
                                               ccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGc                        >  1:433689/1‑71 (MQ=255)
                                                  tttCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCt                     >  1:5273455/1‑71 (MQ=255)
                                                   ttCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTg                    >  1:5572796/1‑71 (MQ=255)
                                                    tCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCAtt                                           <  1:5260805/47‑1 (MQ=255)
                                                    tCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtct                                   >  1:5192297/1‑55 (MQ=255)
                                                    tCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtc                                    >  1:3790962/1‑54 (MQ=255)
                                                       aTAAACGCGGTTTTGACATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAAc                >  1:3670230/1‑71 (MQ=255)
                                                       aTAAACGCGGTTTTGACATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAAc                >  1:3663530/1‑71 (MQ=255)
                                                        tAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAg                            >  1:3800223/1‑58 (MQ=255)
                                                          aaCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCa             <  1:4018471/71‑1 (MQ=255)
                                                            cgcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACt                                       >  1:4705379/1‑43 (MQ=255)
                                                            cgcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACt                                       >  1:4415560/1‑43 (MQ=255)
                                                             gcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtct                                   >  1:1380603/1‑46 (MQ=255)
                                                             gcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtct                                   >  1:922753/1‑46 (MQ=255)
                                                              cgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCg         <  1:2474521/71‑1 (MQ=255)
                                                                  tttGCCATCGCCGGAGATCCCCCAACTGGCATTCACttt                                     <  1:2655758/39‑1 (MQ=255)
                                                                   ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAg                            >  1:976787/1‑47 (MQ=255)
                                                                   ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGcc                       >  1:4816747/1‑52 (MQ=255)
                                                                   ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGa      <  1:660612/69‑1 (MQ=255)
                                                                   ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGa      <  1:4347880/69‑1 (MQ=255)
                                                                   ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGAc     <  1:1676490/70‑1 (MQ=255)
                                                                   ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGAc     <  1:3019747/70‑1 (MQ=255)
                                                                   ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACg    >  1:3011316/1‑71 (MQ=255)
                                                                   ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTATCGCCCAGCGGCCCTGTAACCCAGGCGTGa      <  1:5476708/69‑1 (MQ=255)
                                                                   ttGCCAGCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACg    >  1:737594/1‑71 (MQ=255)
                                                                    tGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGt   >  1:1211212/1‑71 (MQ=255)
                                                                    tGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGt   >  1:5274956/1‑71 (MQ=255)
                                                                     gCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACg    >  1:592681/1‑69 (MQ=255)
                                                                     gCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGtt  >  1:4407448/1‑71 (MQ=255)
                                                                     |                                                                      
TGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTT  >  Exported/2096540‑2096679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: