Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,104,165 G→A 18.4% Q55Q (CAG→CAA kbaZ → tagatose 6‑phosphate aldolase 1, kbaZ subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,104,1650GA18.4% 247.4 / 43.8 114Q55Q (CAG→CAAkbaZtagatose 6‑phosphate aldolase 1, kbaZ subunit
Reads supporting (aligned to +/- strand):  ref base G (45/48);  new base A (16/5);  total (61/53)
Fisher's exact test for biased strand distribution p-value = 2.86e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 124 total aligned reads displayed.

CTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTG  >  Exported/2104095‑2104234
                                                                      |                                                                     
cTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAg                                                                       <  1:5440345/71‑1 (MQ=255)
   cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc                                                                    <  1:2613918/71‑1 (MQ=255)
   cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc                                                                    >  1:4476016/1‑71 (MQ=255)
   cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc                                                                    >  1:1552048/1‑71 (MQ=255)
     aTCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcgg                                                                  <  1:5047803/71‑1 (MQ=255)
      tCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggc                                                                 >  1:3645520/1‑71 (MQ=255)
      tCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggc                                                                 <  1:183417/71‑1 (MQ=255)
        cGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggcgg                                                               >  1:3361451/1‑71 (MQ=255)
        cGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggcg                                                                <  1:2947930/70‑1 (MQ=255)
         gCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGt                                                              <  1:390094/71‑1 (MQ=255)
           tACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta                                                            >  1:1221334/1‑71 (MQ=255)
            actccAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta                                                            >  1:4658344/4‑70 (MQ=255)
                 aGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCgg                                                      <  1:4261789/71‑1 (MQ=255)
                   tGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGaa                                                    <  1:2162299/71‑1 (MQ=255)
                     caatccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGTTAGACCAGTTCGGCGGTTATACCGGAAt                                                   <  1:2460635/70‑1 (MQ=255)
                     caaaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt                                                   >  1:1291697/1‑70 (MQ=255)
                      aaaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGa                                                 <  1:961907/71‑1 (MQ=255)
                       aaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta                                                            >  1:1162522/1‑59 (MQ=255)
                        accaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcg                                                                   >  1:268734/1‑51 (MQ=255)
                          caaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgcc                                             >  1:4833883/1‑71 (MQ=255)
                             acGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtata                                                          >  1:5337673/1‑55 (MQ=255)
                              cGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgcccgcc                                         >  1:2964781/1‑71 (MQ=255)
                              cGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgcccgcc                                         >  1:2849938/1‑71 (MQ=255)
                                 cGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAt                                      >  1:688022/1‑71 (MQ=255)
                                  gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt                                     <  1:4670184/71‑1 (MQ=255)
                                  gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt                                     <  1:5237288/71‑1 (MQ=255)
                                  gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt                                     <  1:4663429/71‑1 (MQ=255)
                                   ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAg                                                                       <  1:872318/36‑1 (MQ=255)
                                   ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAg                                                                       <  1:819041/36‑1 (MQ=255)
                                   ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGt                                                              <  1:5040655/45‑1 (MQ=255)
                                   ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGt                                                              <  1:2628446/45‑1 (MQ=255)
                                   ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtata                                                          <  1:5157905/49‑1 (MQ=255)
                                   ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta                                                            <  1:3134208/47‑1 (MQ=255)
                                   ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAttt                                    <  1:4517395/71‑1 (MQ=255)
                                   ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt                                     <  1:571206/70‑1 (MQ=255)
                                    tgcTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTta                                                            <  1:5381246/44‑1 (MQ=255)
                                    tgcTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGActtt                                   >  1:1452742/3‑71 (MQ=255)
                                    tACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtata                                                          <  1:4972172/48‑1 (MQ=255)
                                    tACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCg                                                       <  1:1515090/51‑1 (MQ=255)
                                     aCTGATTGAAGCAACCTCCAATCAGGTAGACCAga                                                                      >  1:3201554/1‑34 (MQ=255)
                                     aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggcg                                                                >  1:3768371/1‑41 (MQ=255)
                                     aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc                                                                    >  1:4137225/1‑37 (MQ=255)
                                     aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc                                                                    >  1:5181723/1‑37 (MQ=255)
                                     aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtata                                                          >  1:3605181/1‑47 (MQ=255)
                                     aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCAGCCGATTTTc                                  >  1:3781488/1‑71 (MQ=255)
                                      cTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTtata                                                          >  1:2236531/1‑46 (MQ=255)
                                      cTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTtata                                                          >  1:4198216/1‑46 (MQ=255)
                                      cTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTcg                                 >  1:4115025/1‑71 (MQ=255)
                                       tGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGActt                                    <  1:4642488/67‑1 (MQ=255)
                                        gATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTcgcg                               >  1:1766241/1‑71 (MQ=255)
                                         aTTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATAcc                                                        <  1:4982771/45‑1 (MQ=255)
                                         aTTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt                                                   <  1:1889668/50‑1 (MQ=255)
                                          ttGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaa                             >  1:2350933/1‑69 (MQ=255)
                                          ttGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaa                             >  1:2560392/1‑69 (MQ=255)
                                          ttGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt                                                   <  1:2531918/49‑1 (MQ=255)
                                           tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCgg                                                      >  1:2220899/1‑45 (MQ=255)
                                           tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat                            >  1:2493561/1‑68 (MQ=255)
                                           tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat                            >  1:4235724/1‑68 (MQ=255)
                                           tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat                            >  1:5112299/1‑68 (MQ=255)
                                           tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat                            >  1:3330137/1‑68 (MQ=255)
                                           tGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtat                                                           <  1:4668275/40‑1 (MQ=255)
                                           tGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtat                                                           <  1:4066572/40‑1 (MQ=255)
                                           tGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACTCCCGCCGATTTTCGCgg                              >  1:189999/1‑69 (MQ=255)
                                            gAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta                                                            >  1:2316795/1‑38 (MQ=255)
                                              aGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGTAATGACACCGGCAGACTTTCGCGAAtttg                         <  1:1525802/71‑1 (MQ=255)
                                              aGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta                                                            >  1:1977596/1‑36 (MQ=255)
                                              aGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta                                                            >  1:1862434/1‑36 (MQ=255)
                                              aGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta                                                            >  1:1416292/1‑36 (MQ=255)
                                                cAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGaa                                                    >  1:2724506/1‑42 (MQ=255)
                                                cAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGaa                                                    >  1:1464212/1‑42 (MQ=255)
                                                 aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGa                                                 >  1:3224518/1‑44 (MQ=255)
                                                 aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGa                                                 >  1:491660/1‑44 (MQ=255)
                                                 aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAAtttgttt                      <  1:4287668/71‑1 (MQ=255)
                                                 aaCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt                      <  1:5089189/71‑1 (MQ=255)
                                                 aaCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt                      <  1:3228111/71‑1 (MQ=255)
                                                  aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgc                                              >  1:982795/1‑46 (MQ=255)
                                                  aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAt                                      <  1:959601/54‑1 (MQ=255)
                                                  aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAt                                      <  1:4117836/54‑1 (MQ=255)
                                                  aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgtttg                     <  1:1969733/71‑1 (MQ=255)
                                                  aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgt                        >  1:4174387/1‑68 (MQ=255)
                                                  aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgt                        >  1:3450725/1‑68 (MQ=255)
                                                   ccTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt                                                   <  1:5025624/40‑1 (MQ=255)
                                                   ccTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTttgtttgt                    <  1:4330402/71‑1 (MQ=255)
                                                    cTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttg                         <  1:5583957/65‑1 (MQ=255)
                                                    cTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTc                   <  1:3011683/71‑1 (MQ=255)
                                                     tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgccc                                            >  1:5458015/1‑45 (MQ=255)
                                                     tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCt                            >  1:220938/1‑61 (MQ=255)
                                                     tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCt                            >  1:5653725/1‑61 (MQ=255)
                                                         aTCAGGTAGACCAGTTCGGCGGTTATACCGGAATGa                                                 >  1:953726/1‑36 (MQ=255)
                                                          tCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttg                         <  1:5314207/59‑1 (MQ=255)
                                                           cAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGc            >  1:3287018/1‑71 (MQ=255)
                                                            aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTcgc                                >  1:4649050/1‑50 (MQ=255)
                                                            aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGcc           >  1:4632385/1‑71 (MQ=255)
                                                            aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGcc           >  1:1520900/1‑71 (MQ=255)
                                                              gTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt                      <  1:1211817/58‑1 (MQ=255)
                                                               tAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGa         >  1:5044543/1‑70 (MQ=255)
                                                                  aCCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGc                             <  1:4646609/47‑1 (MQ=255)
                                                                     aGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTg  <  1:1085077/71‑1 (MQ=255)
                                                                      gTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt                      <  1:1050824/50‑1 (MQ=255)
                                                                      gTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCTCCGACTCGTTg  >  1:5497384/1‑70 (MQ=255)
                                                                      |                                                                     
CTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTG  >  Exported/2104095‑2104234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: