Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,130,576 C→T 14.4% S110N (AGT→AAT)  mtr ← tryptophan transporter of high affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,130,5760CT14.4% 237.8 / 25.9 97S110N (AGT→AAT) mtrtryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base C (35/48);  new base T (3/11);  total (38/59)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 101 total aligned reads displayed.

AAACCCGCCGCCCGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTC  >  Exported/2130510‑2130644
                                                                  |                                                                    
aaaccCGCCGCCCGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGCACCACTgg                                                                   >  1:596273/1‑70 (MQ=255)
aaaccCGCCGCCCGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTgg                                                                   >  1:1497140/1‑70 (MQ=255)
  accCGCCGCCCGTGCCGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGa                                                               <  1:2547403/71‑1 (MQ=255)
     cgccgcCCGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGaaa                                                             <  1:5522184/71‑1 (MQ=255)
      gccgccCGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAat                                                            <  1:3765078/71‑1 (MQ=255)
           ccGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGCTGCAGAATCGAACCACTGGCAGAAATATAgg                                                       <  1:5222305/71‑1 (MQ=255)
           ccGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGaaa                                                             >  1:5519024/1‑65 (MQ=255)
           ccGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAgg                                                       <  1:4635535/71‑1 (MQ=255)
           ccGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAgg                                                       <  1:4498647/71‑1 (MQ=255)
             gTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAgg                                                       <  1:455288/69‑1 (MQ=255)
                ccGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAg                                                  >  1:1114316/1‑71 (MQ=255)
                 cGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAgg                                                 >  1:3219683/1‑71 (MQ=255)
                     aCGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg                                             >  1:1720400/1‑71 (MQ=255)
                        tttAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTgg                                                                   <  1:1056247/46‑1 (MQ=255)
                        tttAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTgg                                                                   <  1:4526633/46‑1 (MQ=255)
                          tAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAg                                                  >  1:3415538/1‑61 (MQ=255)
                          tAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAg                                                  >  1:4870407/1‑61 (MQ=255)
                            gTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGc                                                      <  1:5387522/55‑1 (MQ=255)
                            gTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGc                                                      <  1:4085446/55‑1 (MQ=255)
                            gTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATag                                      <  1:4904743/71‑1 (MQ=255)
                              gACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAg                                                        >  1:2422352/1‑51 (MQ=255)
                               aCATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATagag                                    <  1:1318336/70‑1 (MQ=255)
                               aCATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATagag                                    <  1:4527283/70‑1 (MQ=255)
                               aCATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATaga                                     >  1:309727/1‑69 (MQ=255)
                                cATCTCTGCGAATGTGTGATGCAGAATCGAACCACTGGCAGAAat                                                            <  1:3749111/45‑1 (MQ=255)
                                cATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAat                                                            <  1:2700391/45‑1 (MQ=255)
                                cATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAat                                                            <  1:3082511/45‑1 (MQ=255)
                                cATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAat                                                            <  1:1089298/45‑1 (MQ=255)
                                cATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAat                                                            <  1:4657528/45‑1 (MQ=255)
                                cATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAat                                                            <  1:5214780/45‑1 (MQ=255)
                                  tctcTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGat                                          <  1:730225/61‑1 (MQ=255)
                                  tctcTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGat                                          <  1:2468780/61‑1 (MQ=255)
                                    tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAgg                                                 >  1:2008580/1‑52 (MQ=255)
                                    tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAgg                                                 >  1:1716750/1‑52 (MQ=255)
                                    tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGatat                                        >  1:1961994/1‑61 (MQ=255)
                                    tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGatat                                        >  1:1395357/1‑61 (MQ=255)
                                    tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGatat                                        >  1:4725327/1‑61 (MQ=255)
                                    tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGatat                                        >  1:1895592/1‑61 (MQ=255)
                                    tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGat                                          <  1:1504521/59‑1 (MQ=255)
                                    tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGat                                          <  1:4985590/59‑1 (MQ=255)
                                    tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca                                >  1:5024971/1‑69 (MQ=255)
                                    tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca                                >  1:4181047/1‑69 (MQ=255)
                                      tGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca                                <  1:900020/67‑1 (MQ=255)
                                      tGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACaa                               <  1:3376644/68‑1 (MQ=255)
                                        cGAAGGTGTGATGCAGAATCGAACCACTGGCAGaa                                                              >  1:4558262/1‑35 (MQ=255)
                                        cGAAGGTGTGATGCAGAATCGAACCACTGGCAGaa                                                              >  1:1128755/1‑35 (MQ=255)
                                        cGAAGGTGTGATGCAGAATCGAACCACTGGCAGaa                                                              >  1:4770016/1‑35 (MQ=255)
                                        cGAAGGTGTGATGCAGAATCGAACCACTGGCAGaa                                                              >  1:1254031/1‑35 (MQ=255)
                                          aaGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAAt                        <  1:1850097/71‑1 (MQ=255)
                                            ggTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATgg                      <  1:2725584/71‑1 (MQ=255)
                                            ggTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATgg                      <  1:635539/71‑1 (MQ=255)
                                             gtgtGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGt                                                <  1:3140290/44‑1 (MQ=255)
                                             gtgtGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGt                                                <  1:1137105/44‑1 (MQ=255)
                                             gtgtGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                     <  1:1183978/71‑1 (MQ=255)
                                              tgtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAg                                             <  1:4441807/46‑1 (MQ=255)
                                              tgtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca                                <  1:2728283/59‑1 (MQ=255)
                                                tgATGCAGAATCGAACCACTGGCAGAAATATAGGCAt                                                    <  1:2806594/37‑1 (MQ=255)
                                                tgATGCAGAATCGAACCACTGGCAGAAATATAGGCAt                                                    <  1:5692294/37‑1 (MQ=255)
                                                  aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAAt                        >  1:838448/1‑63 (MQ=255)
                                                  aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc                >  1:3914307/1‑71 (MQ=255)
                                                   tGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGata                                         <  1:1572467/45‑1 (MQ=255)
                                                   tGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGata                                         <  1:4511095/45‑1 (MQ=255)
                                                     cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATgg                      <  1:3606152/62‑1 (MQ=255)
                                                      aGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt            <  1:2573442/71‑1 (MQ=255)
                                                      aGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt            <  1:5352501/71‑1 (MQ=255)
                                                      aGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt            <  1:4577236/71‑1 (MQ=255)
                                                       tgaTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACgaa                              >  1:4315429/3‑52 (MQ=255)
                                                       tgaTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACgaa                              >  1:2113700/3‑52 (MQ=255)
                                                       tgaTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACggt                     <  1:4399599/59‑2 (MQ=255)
                                                       tgaTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGtaa                   <  1:2464514/61‑1 (MQ=255)
                                                       gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                     <  1:2594640/61‑1 (MQ=255)
                                                       gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                     <  1:126007/61‑1 (MQ=255)
                                                       gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                     <  1:814313/61‑1 (MQ=255)
                                                       gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt            >  1:4628389/1‑70 (MQ=255)
                                                        gaTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAg                             >  1:1433660/2‑52 (MQ=255)
                                                         aTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATag                                      <  1:545485/42‑1 (MQ=16)
                                                         aTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGat         <  1:4250176/71‑2 (MQ=255)
                                                         aTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGat         <  1:1718479/71‑2 (MQ=255)
                                                         aTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGat         <  1:5186317/71‑2 (MQ=255)
                                                         aTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGat         <  1:4173012/71‑2 (MQ=255)
                                                         aTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGat         <  1:1227092/71‑2 (MQ=255)
                                                          tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaa                    <  1:1585121/59‑1 (MQ=255)
                                                          tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc         >  1:4458215/1‑70 (MQ=255)
                                                          tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc         >  1:2605545/1‑70 (MQ=255)
                                                          tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc         >  1:2932335/1‑70 (MQ=255)
                                                           cGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaaa                   >  1:2291876/1‑59 (MQ=255)
                                                           cGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaaa                   >  1:4121676/1‑59 (MQ=255)
                                                           cGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaaa                   >  1:3769785/1‑59 (MQ=255)
                                                           cGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaaa                   >  1:3640919/1‑59 (MQ=255)
                                                            gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCa       >  1:1514809/1‑70 (MQ=255)
                                                            gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCAc      >  1:1087905/1‑71 (MQ=255)
                                                              aCCATTAGCAGAGATATAGGCATAAGTGATGATATAGAGAACGAAGGCAACggt                     <  1:2002172/54‑2 (MQ=255)
                                                              aCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGc                           <  1:2124470/48‑1 (MQ=255)
                                                              aCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACggt                     <  1:2605608/54‑2 (MQ=255)
                                                               ccACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGtt   <  1:648042/71‑1 (MQ=255)
                                                                cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTc  <  1:4121138/71‑1 (MQ=255)
                                                                  cTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCa       >  1:1605069/1‑64 (MQ=255)
                                                                  cTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt    <  1:1466945/67‑1 (MQ=255)
                                                                  cTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt    <  1:2089049/67‑1 (MQ=255)
                                                                  cTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTc  >  1:2427702/1‑69 (MQ=255)
                                                                  |                                                                    
AAACCCGCCGCCCGTGCCGGGACGTTTAGTGACATCTCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTC  >  Exported/2130510‑2130644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: