Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,130,648 C→G 11.2% G86A (GGC→GCC) ‡ mtr ← tryptophan transporter of high affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,130,6480CG11.2% 285.0 / 22.6 107G86A (GGC→GCC) ‡mtrtryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base C (41/54);  new base G (5/7);  total (46/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 114 total aligned reads displayed.

GGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCA  >  Exported/2130578‑2130717
                                                                      |                                                                     
ggCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc                                                                       >  1:4944555/1‑71 (MQ=255)
 gCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGcc                                                                      >  1:5184729/1‑71 (MQ=255)
       atatAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc                                                                <  1:3590723/71‑1 (MQ=255)
       atatAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc                                                                >  1:1800618/1‑71 (MQ=255)
        tataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGccc                                                               <  1:2750719/71‑1 (MQ=255)
         ataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGccc                                                               <  1:3340983/70‑1 (MQ=255)
          taGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAg                                                             >  1:17965/1‑71 (MQ=255)
             gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCa                                                           >  1:2994315/1‑70 (MQ=255)
                tAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc                                                       >  1:389185/1‑71 (MQ=255)
                 aGGTCAGGATATAGAGCACAAAGGCGATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                                                      >  1:2905092/1‑71 (MQ=255)
                  ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCttt                                                                   <  1:1562379/57‑1 (MQ=255)
                   gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                                                     <  1:4258585/70‑1 (MQ=255)
                     cAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCCGCAAATCTTTg                                                   >  1:2796383/1‑70 (MQ=255)
                      aGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGt                                                 >  1:3723559/1‑71 (MQ=255)
                       ggATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTg                                                <  1:4305190/71‑1 (MQ=255)
                        gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                                               >  1:5392089/1‑71 (MQ=255)
                        gATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGa                                               >  1:3970433/1‑71 (MQ=255)
                         atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt                                              <  1:2089740/71‑1 (MQ=255)
                          tataGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATg                                             >  1:1344534/1‑71 (MQ=255)
                            taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTg                                                <  1:841671/66‑1 (MQ=255)
                            taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTg                                                >  1:1008746/1‑66 (MQ=255)
                            taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                                               <  1:5029024/67‑1 (MQ=255)
                            taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                                               <  1:5022463/67‑1 (MQ=255)
                              gagCACATAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                                               <  1:2824126/65‑1 (MQ=255)
                              gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCATATCttt                                                    <  1:11791/60‑1 (MQ=255)
                              gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                                                    <  1:4053417/60‑1 (MQ=255)
                              gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                                                    <  1:1146724/60‑1 (MQ=255)
                              gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                                                    <  1:1267016/60‑1 (MQ=255)
                              gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtg                                          >  1:836607/1‑70 (MQ=255)
                                gCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg                                                  >  1:4798413/1‑60 (MQ=255)
                                 cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                                      <  1:2050087/71‑1 (MQ=255)
                                 cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                                      <  1:4613688/71‑1 (MQ=255)
                                 cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                                      <  1:3111314/71‑1 (MQ=255)
                                  acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa                                      <  1:5010047/67‑1 (MQ=255)
                                  acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa                                      <  1:5535236/67‑1 (MQ=255)
                                  acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCttt                                                                   <  1:2357421/41‑1 (MQ=255)
                                  acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                                      <  1:3226932/70‑1 (MQ=255)
                                   cgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaaaa                                    >  1:190574/3‑71 (MQ=255)
                                   cgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaaaa                                    <  1:223424/69‑1 (MQ=255)
                                   cgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaaaa                                    >  1:1372428/3‑71 (MQ=255)
                                   caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATg                                             <  1:3569765/62‑1 (MQ=255)
                                   caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa                                    <  1:4942605/71‑1 (MQ=255)
                                     aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa                                    >  1:2756128/1‑69 (MQ=255)
                                      aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt                                                        >  1:3630603/1‑48 (MQ=255)
                                      aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt                                                        >  1:5668755/1‑48 (MQ=255)
                                      aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt                                                        >  1:5260916/1‑48 (MQ=255)
                                      aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc                                                       <  1:1220998/49‑1 (MQ=255)
                                      aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc                                                       <  1:721850/49‑1 (MQ=255)
                                      aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc                                                       <  1:4152227/49‑1 (MQ=255)
                                      aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc                                 >  1:4489128/1‑71 (MQ=255)
                                      aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc                                 <  1:2801628/71‑1 (MQ=255)
                                      aGGCAATGGAAATGCCGTTAACCACGTTCCAGCCTTTGCCCAGCAAAt                                                        >  1:2848161/1‑48 (MQ=255)
                                      aGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgtgtta                                       >  1:4137638/1‑63 (MQ=255)
                                      aGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTAAAACtg                                 <  1:3774747/71‑2 (MQ=255)
                                       ggCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTAAAACtgg                                <  1:2852568/71‑3 (MQ=255)
                                        gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                                                   >  1:2372887/1‑51 (MQ=255)
                                        gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                                               >  1:4735662/1‑55 (MQ=255)
                                        gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                                               >  1:3868890/1‑55 (MQ=255)
                                          aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                                                     <  1:3966389/47‑1 (MQ=255)
                                          aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                                                 >  1:5610506/1‑51 (MQ=255)
                                          aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                                                 >  1:4753208/1‑51 (MQ=255)
                                          aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                                                 >  1:5439009/1‑51 (MQ=255)
                                          aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                                                 >  1:940966/1‑51 (MQ=255)
                                           aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaaa                                                         >  1:1236361/1‑42 (MQ=255)
                                           aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaaa                                                         >  1:5349269/1‑42 (MQ=255)
                                           aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                                                      <  1:3890396/45‑1 (MQ=255)
                                           aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                                                      <  1:4840014/45‑1 (MQ=255)
                                           aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                                                      <  1:2854481/45‑1 (MQ=255)
                                           aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg                                                  <  1:1938430/49‑1 (MQ=255)
                                           aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg                                                  <  1:1941406/49‑1 (MQ=255)
                                           aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg                                                  <  1:1796894/49‑1 (MQ=255)
                                            tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                                                     >  1:644446/1‑45 (MQ=255)
                                            tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                                                   >  1:3599880/1‑47 (MQ=255)
                                            tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                                                 <  1:1357031/49‑1 (MQ=255)
                                             ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGc                                                            >  1:883130/1‑37 (MQ=255)
                                             ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGc                                                            >  1:3105797/1‑37 (MQ=255)
                                             ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCAAGCAAATCttt                                                    >  1:1344116/1‑45 (MQ=255)
                                               aaaTGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGAtt                        <  1:4854646/71‑1 (MQ=255)
                                               aaaTGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGAtt                        >  1:2521635/1‑71 (MQ=255)
                                                aaTGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCg                                <  1:2759106/62‑1 (MQ=255)
                                                  tGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                                      <  1:1296079/54‑1 (MQ=255)
                                                  tGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTc                       <  1:1520045/69‑1 (MQ=255)
                                                  tGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTc                       <  1:2190586/69‑1 (MQ=255)
                                                   gCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTg                     >  1:4191754/1‑70 (MQ=255)
                                                    ccGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGt                    >  1:5087710/1‑70 (MQ=255)
                                                    ccGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGt                    <  1:3230345/70‑1 (MQ=255)
                                                     cGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                                      <  1:268737/51‑1 (MQ=255)
                                                      gTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa                                    >  1:3957565/1‑52 (MQ=255)
                                                      gTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc                            <  1:3655996/60‑1 (MQ=255)
                                                      gTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc                            <  1:2726522/60‑1 (MQ=255)
                                                         gACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTc               <  1:126705/70‑1 (MQ=255)
                                                            cACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGt           >  1:869825/1‑71 (MQ=255)
                                                              cGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGt           >  1:2991543/1‑69 (MQ=255)
                                                              cGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTa         <  1:1545702/71‑1 (MQ=255)
                                                              cGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTa         <  1:3566885/71‑1 (MQ=255)
                                                                     gCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCa  <  1:481581/71‑1 (MQ=255)
                                                                      ccTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTc               >  1:3424217/1‑57 (MQ=255)
                                                                      ccTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCa              >  1:2062936/1‑58 (MQ=255)
                                                                      ccTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCa              >  1:4726282/1‑58 (MQ=255)
                                                                      ccTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCa              >  1:3231600/1‑58 (MQ=255)
                                                                      |                                                                     
GGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCA  >  Exported/2130578‑2130717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: