| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,155,567 | A→T | 19.3% | intergenic (+78/+108) | dacB → / ← obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,155,567 | 0 | A | T | 19.3% | 98.8 / 24.9 | 57 | intergenic (+78/+108) | dacB/obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Reads supporting (aligned to +/- strand): ref base A (26/20); new base T (5/6); total (31/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATA > Exported/2155497‑2155633 | gAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:3823536/71‑1 (MQ=255) ggCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCt > 1:4772423/1‑70 (MQ=255) acaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc > 1:3364822/1‑71 (MQ=255) acaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc > 1:4800180/1‑71 (MQ=255) caTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:1301565/59‑1 (MQ=255) tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:1927413/57‑1 (MQ=255) tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:2977235/57‑1 (MQ=255) tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:2921788/57‑1 (MQ=255) gCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc < 1:4255506/70‑1 (MQ=255) cggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc > 1:3673691/2‑70 (MQ=11) ggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa < 1:1553601/71‑1 (MQ=11) ggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa > 1:3050819/1‑70 (MQ=11) ggCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa > 1:5520019/1‑70 (MQ=255) cTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa < 1:3245084/71‑1 (MQ=11) cTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa < 1:3841057/71‑1 (MQ=11) cTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa < 1:3285390/71‑1 (MQ=255) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt < 1:3603031/52‑1 (MQ=11) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa < 1:4236095/68‑1 (MQ=11) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa < 1:1356754/68‑1 (MQ=11) cAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCg > 1:4382492/1‑71 (MQ=255) aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa > 1:1573383/1‑46 (MQ=255) attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg > 1:474442/1‑70 (MQ=255) tgttGACAACGTACGCATTGTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAACAGTGCa > 1:534654/3‑70 (MQ=11) tgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa > 1:4728969/3‑49 (MQ=11) tgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc > 1:339141/3‑69 (MQ=255) tgctGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCa > 1:4908578/4‑70 (MQ=11) gctGACAAATTGCGCTTTATGT‑TGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAt > 1:2276917/3‑71 (MQ=11) ttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc < 1:2908284/57‑1 (MQ=40) ctGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAAt > 1:4521451/2‑71 (MQ=255) tGACAAATTGCGCTTTATGT‑TGCCGGATGCGGCGTAAACGCCTTATcc > 1:4614147/1‑48 (MQ=11) tGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTa < 1:3205179/46‑1 (MQ=40) aaaCAAAGCGCACTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt > 1:297689/3‑71 (MQ=11) gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt > 1:1553547/1‑70 (MQ=255) aCAAATTGCGCTTTATGT‑TGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa > 1:4820455/1‑71 (MQ=11) aCAAATTGCGCTTTATGT‑TGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa > 1:2350845/1‑71 (MQ=11) aCAAATTGCGCTTTATGT‑TGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa > 1:4762957/1‑71 (MQ=11) aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc > 1:1072952/1‑70 (MQ=39) aacaGTGCGCTTTGTTTATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCaa > 1:4747307/4‑71 (MQ=11) aaaGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCaat < 1:422614/71‑2 (MQ=255) aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaatac < 1:4900152/71‑4 (MQ=9) tGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa > 1:2710053/1‑66 (MQ=255) tGCGCTTTGTTTATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCaata < 1:4553164/68‑3 (MQ=11) tGCGCTTTGTTTATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCaata < 1:4563233/68‑3 (MQ=11) cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCa < 1:970542/55‑1 (MQ=255) ttGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAAt > 1:1546328/1‑71 (MQ=255) tGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat > 1:5692738/1‑64 (MQ=40) tGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat > 1:276101/1‑64 (MQ=40) aTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:2334484/54‑1 (MQ=255) tGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:4561491/71‑1 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc > 1:444215/1‑56 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCa > 1:3612334/1‑57 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc‑t < 1:891784/60‑1 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCt < 1:5120457/71‑1 (MQ=255) cggcgTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCt > 1:253713/1‑70 (MQ=11) cggcgTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg < 1:2459078/71‑1 (MQ=14) cgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:695466/59‑1 (MQ=255) cgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGa > 1:2469852/1‑68 (MQ=255) cgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGAt > 1:2812655/1‑69 (MQ=255) cgTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa < 1:1772080/71‑1 (MQ=14) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAAt < 1:614982/35‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc < 1:3992907/37‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc < 1:5697768/47‑1 (MQ=255) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:1200361/57‑1 (MQ=255) aaaCGCCTTATCCGGCCTACAAAATCGTGCAAATTc < 1:3829410/36‑1 (MQ=40) aaaCGCCTTATCCGGCCTACAAAATCGTGCAAATTc < 1:79329/36‑1 (MQ=40) | GAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATA > Exported/2155497‑2155633 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |