| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,155,638 | T→C | 16.4% | intergenic (+149/+37) | dacB → / ← obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,155,638 | 0 | T | C | 16.4% | 194.7 / 25.4 | 79 | intergenic (+149/+37) | dacB/obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Reads supporting (aligned to +/- strand): ref base T (48/18); new base C (4/9); total (52/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
CTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCG > Exported/2155573‑2155708 | cTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAgcg > 1:1525361/1‑70 (MQ=255) aTCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:2995949/1‑70 (MQ=255) aTCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAt > 1:2438123/1‑71 (MQ=11) tCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATc < 1:218615/71‑1 (MQ=11) gCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGc < 1:4763606/71‑1 (MQ=11) gCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGc < 1:2681351/71‑1 (MQ=11) cTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAg > 1:5235795/1‑58 (MQ=255) cTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAg > 1:5233610/1‑58 (MQ=255) tACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGcaat > 1:5363373/1‑68 (MQ=0) aCAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAtt > 1:381283/1‑71 (MQ=255) cataaTCTTGAAAATTCAATATATTGCAGTTA‑TCTGGTAGGCCAGGTAAGCGTAGCGCATc > 1:4678403/4‑61 (MQ=2) cataaTCTTGAAAATTCAATATATTGCAGTTA‑TCTGGTAGGCCAGGTAAGCGTAGCGCATc > 1:3137942/4‑61 (MQ=2) cataaTCTTGAAAATTCAATATATTGCAGTTA‑TCTGGTAGGCCAGGTAAGCGTAGCGCATc > 1:2272322/4‑61 (MQ=2) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAttt > 1:623795/1‑71 (MQ=255) cAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGcaat < 1:1641788/69‑4 (MQ=0) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAttt > 1:1090193/1‑70 (MQ=255) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAttt > 1:1547663/1‑70 (MQ=255) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTg > 1:3810780/1‑71 (MQ=255) aaaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTg > 1:3142566/1‑71 (MQ=255) aaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:2970338/1‑57 (MQ=255) aaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGc > 1:4833084/1‑71 (MQ=255) aatgTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC‑AATTTGGCg < 1:4549032/68‑1 (MQ=2) tCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAtt < 1:1816328/65‑1 (MQ=255) cAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCa < 1:830321/53‑1 (MQ=11) cAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC‑AATTTGGCGttgcc < 1:1749085/70‑4 (MQ=2) aaaTTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC‑AATTTGGCGtt < 1:163315/66‑1 (MQ=2) aaaTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatca > 1:4118040/1‑71 (MQ=255) aaaTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatca > 1:3386257/1‑71 (MQ=255) aaTTCAATAAATTGCAATTCATCTTGTAGGCCTGATAAGCGCAGCGCATCAGGC‑AATTTGGCGtt < 1:2665475/65‑1 (MQ=11) aTTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGcaa > 1:3344038/1‑53 (MQ=0) aTTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC‑AATTTGGCGttgc > 1:1678013/1‑64 (MQ=2) aTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatcat > 1:4919494/1‑70 (MQ=255) aTTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatcat > 1:5502185/1‑70 (MQ=255) ttCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCt < 1:1600126/52‑1 (MQ=255) tCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAg > 1:5107934/1‑71 (MQ=255) tCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAg > 1:5525137/1‑71 (MQ=255) cATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTa < 1:1990295/60‑1 (MQ=255) cATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTa < 1:5125964/60‑1 (MQ=255) atatTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGAtt < 1:1322188/71‑1 (MQ=255) atTGCAATGC‑ACTTGTAGGCCTGATAAGCGTAGCGCa < 1:2561492/37‑1 (MQ=14) atTGCAATGC‑ACTTGTAGGCCTGATAAGCGTAGCGCa < 1:2887096/37‑1 (MQ=14) ttGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAAc > 1:2779820/1‑71 (MQ=255) gCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCa > 1:422365/1‑37 (MQ=255) gCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCa > 1:3699390/1‑37 (MQ=255) aaTTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGa < 1:3559613/41‑1 (MQ=255) ttC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatcat < 1:2978480/54‑1 (MQ=255) tc‑tcTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCt > 1:4014597/1‑67 (MQ=255) c‑tctTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGt > 1:2199740/1‑69 (MQ=255) c‑tctTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTa > 1:368561/1‑70 (MQ=255) c‑tctTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTa > 1:1341972/1‑70 (MQ=255) c‑tctTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTa > 1:4052852/1‑70 (MQ=255) ctTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTatca < 1:3920681/49‑1 (MQ=255) ttGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTaa > 1:1400714/1‑59 (MQ=255) ttGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTaa < 1:1620921/68‑1 (MQ=255) ttGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTaa < 1:1140017/68‑1 (MQ=255) aGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAAc > 1:653652/1‑70 (MQ=255) aGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACt > 1:5032469/1‑71 (MQ=255) ggCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTc < 1:5274481/71‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCa < 1:5155150/71‑1 (MQ=255) tGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCa < 1:3616268/68‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCTGATTTGGCGTTTAtcatca > 1:5010749/1‑40 (MQ=255) aTAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGt < 1:1475873/42‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGt < 1:2800478/42‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGAtt > 1:4029900/1‑46 (MQ=255) aTAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTa > 1:813473/1‑47 (MQ=255) aTAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGcc > 1:1989766/1‑71 (MQ=255) tAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATg < 1:4430770/59‑1 (MQ=255) tAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCct > 1:5529151/1‑71 (MQ=255) aaGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGa > 1:4503272/1‑42 (MQ=255) aaGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGa > 1:5526966/1‑42 (MQ=255) aGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGaa > 1:4364609/1‑59 (MQ=255) aGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGaa > 1:1988480/1‑59 (MQ=255) aGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGa > 1:2557608/1‑58 (MQ=255) aGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCcttc > 1:990033/1‑71 (MQ=255) cGTAGCGCATCAGGCTGATTTGGCGTTTAtcatca > 1:5451966/1‑35 (MQ=255) cGTAGCGCATCAGGCTGATTTGGCGTTTAtcatca > 1:4749567/1‑35 (MQ=255) cGTAGCGCATCAGGCTGATTTGGCGTTTAtcatca > 1:1892441/1‑35 (MQ=255) cGTAGCGCATCAGGCTGATTTGGCGTTTAtcatca > 1:896941/1‑35 (MQ=255) gTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTaaa > 1:608927/1‑52 (MQ=255) tAGCGCATCAGGCTGATTTGGCGTTTATCATCAGt < 1:2459959/35‑1 (MQ=255) tAGCGCATCAGGCTGATTTGGCGTTTATCATCAGt < 1:1245485/35‑1 (MQ=255) tAGCGCATCAGGCTGATTTGGCGTTTATCATCAGt < 1:113179/35‑1 (MQ=255) tAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGa > 1:5050123/1‑37 (MQ=255) tAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGa > 1:4166956/1‑37 (MQ=255) tAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGa > 1:3358836/1‑37 (MQ=255) tAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCcttctt > 1:5283982/1‑69 (MQ=255) tAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCttcttc > 1:358710/1‑70 (MQ=255) tAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTtcg < 1:2021258/71‑1 (MQ=255) | CTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGTTTATCATCAGTGATTAACGCTTGTAAATGAACTCAACGCCTTCTTCG > Exported/2155573‑2155708 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |