Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,220,179 G→T 18.0% intergenic (+439/‑539) yhdH → / → accB predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,220,1790GT18.0% 247.5 / 29.2 94intergenic (+439/‑539)yhdH/accBpredicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit
Reads supporting (aligned to +/- strand):  ref base G (35/42);  new base T (8/9);  total (43/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTG  >  Exported/2220109‑2220244
                                                                      |                                                                 
tGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATg                                                                   <  1:918950/71‑1 (MQ=255)
 gCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATgt                                                                  >  1:481108/1‑71 (MQ=255)
 gCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATgt                                                                  >  1:1633661/1‑71 (MQ=255)
  cTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATgtc                                                                 >  1:4470631/1‑71 (MQ=255)
     tAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATgtcgt                                                               <  1:3038778/70‑1 (MQ=255)
         aTTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATc                                                          >  1:2328295/1‑71 (MQ=255)
                tCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCt                                                         >  1:725991/1‑65 (MQ=255)
                 cTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgc                                                   >  1:3169424/1‑70 (MQ=255)
                  aacccAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGa                                                 <  1:4306231/68‑1 (MQ=255)
                  aacccAGGCGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGa                                                 <  1:4653670/68‑1 (MQ=255)
                   aTCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGa                                                 >  1:468223/1‑70 (MQ=255)
                    tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgc                                                   <  1:1828754/67‑1 (MQ=255)
                    tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGa                                                 <  1:5020788/69‑1 (MQ=255)
                    tCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc                                               <  1:4505061/71‑1 (MQ=255)
                      cAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc                                              <  1:4283239/70‑1 (MQ=255)
                       aggcGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTg                                            <  1:3139613/68‑1 (MQ=255)
                       aggcGATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTg                                            >  1:2603526/4‑71 (MQ=255)
                           gATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCAt                                        >  1:2346852/1‑71 (MQ=255)
                           gATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGa                                                 >  1:1681589/1‑62 (MQ=255)
                           gATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGa                                                 >  1:1835493/1‑62 (MQ=255)
                           gATGCAGCGAAAACTCGGCAAGCACTGGCAACAGTTGCATAATTTCGTCTATCTTGTCGCGa                                                 >  1:3365152/1‑62 (MQ=255)
                              gCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                                     >  1:5396563/1‑71 (MQ=255)
                                aGTGAAAATTGGGTAATCCCCAGCTACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTa                                   <  1:475777/71‑1 (MQ=255)
                                aGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTc                                                     >  1:4740542/1‑53 (MQ=255)
                                 gTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTcgcg                                                  <  1:2699941/55‑1 (MQ=255)
                                 gTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCAt                                        <  1:1836574/65‑1 (MQ=255)
                                 gTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCAt                                        <  1:3595115/65‑1 (MQ=255)
                                  tGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc                                              <  1:4539228/58‑1 (MQ=255)
                                  tGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg                                            <  1:4295675/60‑1 (MQ=255)
                                  tGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg                                            <  1:3516440/60‑1 (MQ=255)
                                  tGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg                                            <  1:1083546/60‑1 (MQ=255)
                                   gAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCtt                                                        >  1:1414804/1‑47 (MQ=255)
                                   gAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc                                               >  1:2118056/1‑56 (MQ=255)
                                    aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATgtcg                                                                >  1:2629802/1‑38 (MQ=255)
                                    aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTAtaa                                                         >  1:1496903/1‑43 (MQ=255)
                                    aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTc                                                     >  1:3933827/1‑49 (MQ=255)
                                    aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg                                            >  1:925135/1‑58 (MQ=255)
                                    aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTg                                            >  1:5627965/1‑58 (MQ=255)
                                    aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACat                                >  1:267293/1‑70 (MQ=255)
                                    aaaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGAACCTGGCATCCCTACat                                >  1:4004785/1‑70 (MQ=255)
                                     aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc                                               <  1:3975953/54‑1 (MQ=255)
                                     aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc                                               <  1:577148/54‑1 (MQ=255)
                                     aaaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc                                               <  1:3311709/54‑1 (MQ=255)
                                      aaTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattat                             >  1:3353499/1‑71 (MQ=255)
                                       aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt                            <  1:5607574/71‑1 (MQ=255)
                                       aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt                            <  1:3386335/71‑1 (MQ=255)
                                        ttGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt                            >  1:2948697/1‑70 (MQ=255)
                                        ttGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt                            >  1:2122867/1‑70 (MQ=255)
                                        ttGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTAttt                           <  1:162078/71‑1 (MQ=255)
                                        ttGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTAttt                           <  1:2925540/71‑1 (MQ=255)
                                         tGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCtt                                                        <  1:5149069/41‑1 (MQ=255)
                                         tGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCtt                                                        <  1:4014550/41‑1 (MQ=255)
                                             tAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc                                               <  1:2871434/46‑1 (MQ=255)
                                             tAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATc                                               <  1:1427390/46‑1 (MQ=255)
                                              aaTCCCCAGCAACCGCTGCGTAATGTCGTGGATCTTGTCGCGTTCCTGGCATCCCTACATTTTTTGTgg                       >  1:4540535/1‑69 (MQ=255)
                                              aaTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTAc                                  <  1:3924974/58‑1 (MQ=255)
                                               aTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCt                                             >  1:5601656/1‑46 (MQ=255)
                                               aTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTc                     <  1:1818535/70‑1 (MQ=255)
                                                 ccccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgt                  >  1:627322/1‑71 (MQ=255)
                                                  cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg                 <  1:1736173/71‑1 (MQ=255)
                                                  cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg                 <  1:2005162/71‑1 (MQ=255)
                                                   ctggCAACAGTTGCATAATTTCGTCTATCTTGTGGCGATTCTGGCCCCAATACATTATCTGTGGGCCGTGa                <  1:4910396/68‑1 (MQ=255)
                                                   ctggCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgtg                 >  1:4042929/4‑70 (MQ=255)
                                                   ccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCt                                             <  1:1148480/42‑1 (MQ=255)
                                                    cAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg                 >  1:3741447/1‑69 (MQ=255)
                                                     ggCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgtg                 >  1:3262699/2‑68 (MQ=255)
                                                      gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc                                              >  1:1471143/1‑38 (MQ=255)
                                                      gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATcc                                              >  1:2532016/1‑38 (MQ=255)
                                                      gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                                     <  1:4311493/47‑1 (MQ=255)
                                                      gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                                     <  1:2398680/47‑1 (MQ=255)
                                                      gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                                     <  1:3870149/47‑1 (MQ=255)
                                                      gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATccc                                     <  1:4257752/47‑1 (MQ=255)
                                                      gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGt                      >  1:3018610/1‑62 (MQ=255)
                                                      gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGt                      >  1:673363/1‑62 (MQ=255)
                                                      gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGt                      >  1:5648965/1‑62 (MQ=255)
                                                      gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGa             <  1:1257394/71‑1 (MQ=255)
                                                      gCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgtg                 >  1:1397027/1‑67 (MQ=255)
                                                        aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACatta                              <  1:2460804/52‑1 (MQ=255)
                                                        aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACatta                              <  1:204342/52‑1 (MQ=255)
                                                        aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt           <  1:5545810/71‑1 (MQ=255)
                                                           cGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGc                                          >  1:4422049/1‑37 (MQ=255)
                                                           cGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtc        >  1:2923897/1‑71 (MQ=255)
                                                           cGCTGCGTAATGTCGTCTAACTTGTCGCGATCCTGGc                                          >  1:5650794/1‑37 (MQ=255)
                                                            gCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtct       >  1:4713829/1‑71 (MQ=255)
                                                             ttGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACatta                              <  1:2462908/46‑1 (MQ=255)
                                                             ttGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACatta                              <  1:4317539/46‑1 (MQ=255)
                                                             ttGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACatta                              <  1:5260081/46‑1 (MQ=255)
                                                             ttGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTAtttc      <  1:431565/70‑1 (MQ=255)
                                                             ttGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTAtttc      <  1:2915009/70‑1 (MQ=255)
                                                             cTGCGTAATGTCGTCTATCTTGTCGCGCTCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtct       >  1:4510203/1‑70 (MQ=255)
                                                              tGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg                 <  1:898184/59‑1 (MQ=255)
                                                              tGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtctc      <  1:1517157/70‑1 (MQ=255)
                                                              tGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCa     >  1:5541425/1‑71 (MQ=255)
                                                                cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa     <  1:1943736/69‑1 (MQ=255)
                                                                cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa     <  1:1744959/69‑1 (MQ=255)
                                                                cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCAt    >  1:5020110/1‑70 (MQ=255)
                                                                 gTAATGTCGTCTCTCTTGTCGCGATCCTGGCATCCCTACATTATTTGTTGTCTGTGAAGATTATCTCATTg  <  1:1870458/71‑1 (MQ=255)
                                                                 gTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTtgtg                        >  1:310254/1‑49 (MQ=255)
                                                                 gTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTg  <  1:3085671/71‑1 (MQ=255)
                                                                   aaTGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTg  >  1:917296/1‑69 (MQ=255)
                                                                      |                                                                 
TGCTTTAGCATTCACATCTATCCAGACGATGCAGTGAAAATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTG  >  Exported/2220109‑2220244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: