| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,292,617 | A→G | 56.6% | intergenic (+168/‑253) | fsaB → / → gldA | fructose‑6‑phosphate aldolase 2/glycerol dehydrogenase, NAD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,292,617 | 0 | A | G | 56.6% | 20.1 / 58.2 | 53 | intergenic (+168/‑253) | fsaB/gldA | fructose‑6‑phosphate aldolase 2/glycerol dehydrogenase, NAD |
| Reads supporting (aligned to +/- strand): ref base A (9/14); new base G (18/12); total (27/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TTAGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGG > Exported/2292551‑2292680 | ttaGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACgg > 1:478875/1‑71 (MQ=255) aGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCa > 1:109277/1‑71 (MQ=255) gCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCAt > 1:4643989/1‑71 (MQ=255) gCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCAt > 1:4666674/1‑71 (MQ=255) cACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCg > 1:3221493/1‑70 (MQ=255) aCTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTa < 1:4906419/71‑1 (MQ=255) cTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTa < 1:3646243/70‑1 (MQ=255) tGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCAtt < 1:2752864/65‑1 (MQ=255) ggCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACgg > 1:2838524/1‑71 (MQ=255) ggCAGCGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACgg > 1:4159584/1‑71 (MQ=255) aGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGtt > 1:4577520/1‑70 (MQ=255) aGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCg < 1:2094635/63‑1 (MQ=255) ggCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCg < 1:979456/60‑1 (MQ=255) ggCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCg < 1:2332234/60‑1 (MQ=255) gCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGa < 1:4911629/71‑1 (MQ=255) cAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAg > 1:626140/1‑71 (MQ=255) cAAAATACGTTGCTCCGTATGTTAACCGCGTAGATACCCAGGGCGGAGACGGCATTCGTAc > 1:2389305/1‑61 (MQ=255) aaaaTACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGc > 1:755387/1‑71 (MQ=255) aTACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTg > 1:1216046/1‑70 (MQ=255) aTACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTg > 1:4847586/1‑70 (MQ=255) aTACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTg > 1:3129281/1‑70 (MQ=255) aTACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTa > 1:2180586/1‑56 (MQ=255) cGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGc > 1:291062/1‑68 (MQ=255) cGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGc > 1:4692160/1‑68 (MQ=255) ttGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACg < 1:1335719/71‑1 (MQ=255) ttGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACg < 1:16130/71‑1 (MQ=255) cGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGtt < 1:423262/70‑1 (MQ=255) tATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTg < 1:2054000/57‑1 (MQ=255) tATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGa < 1:5455793/71‑1 (MQ=255) tATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGa < 1:1464674/71‑1 (MQ=255) aTGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGa > 1:4035308/1‑70 (MQ=255) tGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTc < 1:1839936/47‑1 (MQ=255) tGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaa < 1:1549424/70‑1 (MQ=255) tAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATg > 1:1766121/1‑70 (MQ=255) aaCCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGc < 1:3450464/70‑1 (MQ=255) aaCCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCa > 1:3774002/1‑71 (MQ=255) cgcgTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCAcgc < 1:1656347/71‑1 (MQ=255) cgcgTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCAcgc < 1:1545815/71‑1 (MQ=255) gATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaa < 1:2614963/57‑1 (MQ=255) gATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaa < 1:3746414/57‑1 (MQ=255) gATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaa < 1:5138819/57‑1 (MQ=255) tGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaa > 1:3683462/1‑55 (MQ=255) gCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaaa > 1:2227303/1‑55 (MQ=255) cccAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGa < 1:3344495/52‑1 (MQ=255) ccAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCa > 1:5190117/1‑71 (MQ=255) ccAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGc < 1:3146835/70‑1 (MQ=255) ggCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATgg < 1:3948223/70‑1 (MQ=255) ggCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATg > 1:2786859/1‑69 (MQ=255) ggCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATg > 1:5219773/1‑69 (MQ=255) cGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGtt > 1:871882/1‑42 (MQ=255) cGGAGACGGCATTAGTACGGTTCAGGAGCTGCAAACGCTGtt > 1:4125054/1‑42 (MQ=38) cGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAg < 1:5255884/59‑1 (MQ=255) cGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAg < 1:2912937/59‑1 (MQ=255) | TTAGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGG > Exported/2292551‑2292680 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |