| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,294,078 | C→A | 10.9% | intergenic (+105/‑170) | gldA → / → yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,294,078 | 0 | C | A | 10.9% | 359.1 / 17.9 | 126 | intergenic (+105/‑170) | gldA/yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (53/59); new base A (9/5); total (62/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 128 total aligned reads displayed.
CGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGA > Exported/2294010‑2294136 | cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtt < 1:3711802/71‑1 (MQ=255)cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtt < 1:4396510/71‑1 (MQ=255)cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtt < 1:3338977/71‑1 (MQ=255) gCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCttt < 1:4666928/71‑1 (MQ=255) aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGATTTTGCGTGCTTTTg > 1:5198063‑M2/1‑70 (MQ=255) aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg < 1:1393426‑M2/71‑2 (MQ=255) aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg < 1:5487399‑M2/71‑2 (MQ=255) aaatGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:3981576/4‑69 (MQ=255) aatgcCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta > 1:2619056‑M2/3‑67 (MQ=255) tGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGc < 1:889139/71‑1 (MQ=255) tGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGc < 1:1354478/71‑1 (MQ=255) tGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGc < 1:557875/71‑1 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGcag < 1:2398025/71‑3 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgtagg > 1:1562397‑M2/1‑61 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgtagg > 1:223170‑M2/1‑61 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGa > 1:1324775/1‑71 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGa > 1:2831294/1‑71 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGa > 1:4901081/1‑70 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt < 1:4455300/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATa < 1:973445/71‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:4445909‑M2/1‑59 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt > 1:1621580/1‑70 (MQ=255) tGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTgt < 1:2117498‑M2/60‑3 (MQ=255) gcgcTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:5430285‑M2/60‑4 (MQ=255) gcgcTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:900149‑M2/60‑4 (MQ=255) gcgcTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:2471523‑M2/60‑4 (MQ=255) gcgcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGc > 1:1789694/1‑53 (MQ=255) cgcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAg < 1:2057525/70‑1 (MQ=255) cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtttt < 1:5571167/54‑1 (MQ=255) cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtttt < 1:4523524/54‑1 (MQ=255) cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:1730447‑M2/57‑4 (MQ=255) cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:1753831‑M2/57‑4 (MQ=255) cTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCg < 1:1017138/71‑1 (MQ=255) cTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCg < 1:581835/71‑1 (MQ=255) tACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGc < 1:234742/49‑1 (MQ=255) aCGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgt < 1:465315‑M2/54‑3 (MQ=255) cTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCAc > 1:3613057/1‑71 (MQ=255) tATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCg < 1:54883/64‑1 (MQ=255) tATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCg < 1:2644355/64‑1 (MQ=255) aTCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTcc > 1:1376865/1‑71 (MQ=255) tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:1119658‑M2/1‑45 (MQ=255) tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:4811485‑M2/1‑45 (MQ=255) tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGcag > 1:5440119/1‑51 (MQ=255) tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt < 1:2993334/56‑1 (MQ=17) tCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCtttt < 1:2993628/45‑1 (MQ=255) cAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCg > 1:4720792/1‑70 (MQ=255) aGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:1977081/1‑56 (MQ=17) aGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCa < 1:3097563/71‑1 (MQ=255) gCCTACGCCATCTCTGCAATATATTGAATTTGCTTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATc < 1:5565589/71‑1 (MQ=14) gCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATc < 1:350415/71‑1 (MQ=14) cTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCg > 1:3390699/1‑71 (MQ=11) tACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:2706362/57‑1 (MQ=255) tACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:5361768/57‑1 (MQ=255) aCGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:776566/48‑1 (MQ=255) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:4195964/1‑49 (MQ=14) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:2887121/1‑49 (MQ=14) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTc > 1:4768019/1‑56 (MQ=14) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCgg < 1:1258126/69‑1 (MQ=11) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCa > 1:3472742/1‑71 (MQ=11) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCa > 1:4381388/1‑71 (MQ=11) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCa > 1:2113870/1‑71 (MQ=11) cGCAATCTCTGCAATATAATGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCTTCCgg < 1:4109549/69‑1 (MQ=255) tctcTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt < 1:1518826/42‑1 (MQ=255) tctcTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATa < 1:4591468/43‑1 (MQ=255) ctctGCAATATATTGAATTTGCGTGCTTTTGTAAGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCtgt > 1:2420119/1‑71 (MQ=255) tctGCAATATATTGAATTTGCGTGCTTTTGTAGGca > 1:822454/1‑35 (MQ=255) tctGCAATATATTGAATTTGCGTGCTTTTGTAGGca > 1:1899314/1‑35 (MQ=255) tctGCAATATATTGAATTTGCGTGCTTTTGTAGGca > 1:4698597/1‑35 (MQ=255) tctGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCa > 1:4102370/1‑64 (MQ=40) ctGCAATATATTGAATTTGCGTGCTTTTTTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGc > 1:5678167/1‑71 (MQ=255) ctGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:3646079/47‑1 (MQ=38) tGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGc > 1:4100520/1‑70 (MQ=255) cAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCa < 1:2137637/46‑1 (MQ=38) cAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCa < 1:5045231/46‑1 (MQ=38) cAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt < 1:50652/36‑1 (MQ=255) atatatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGt > 1:1044988/1‑70 (MQ=255) atatatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGt < 1:1771631/70‑1 (MQ=255) atatatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTa < 1:2666049/71‑1 (MQ=255) tatTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCa > 1:4161596/1‑41 (MQ=255) atTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCa > 1:704235/1‑40 (MQ=40) ttGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCt > 1:540453/1‑70 (MQ=255) ctgATTTAGCGTGATTTTgtaggtcggataaggcgtt > 1:688064‑M2/2‑18 (MQ=255) tGATTTAGCGTGATTTTgtaggtcggataaggcgttta > 1:3233117‑M2/1‑17 (MQ=255) tGATTTAGCGTGATTTTgtaggtcggataaggcgtt > 1:721931‑M2/1‑17 (MQ=255) tGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTc > 1:5021569/1‑37 (MQ=255) gATTTAGCGTGATTTTgtaggtcggataaggcgtttatgccgc > 1:3013745‑M2/1‑16 (MQ=255) gATTTAGCGTGATTTTgtaggtcggataaggcgtttacgccgca > 1:4543715‑M2/1‑16 (MQ=255) gAATTTGCGTGCTTTTGTAGGCCTGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGa < 1:770221/70‑1 (MQ=255) gAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGa < 1:2242911/70‑1 (MQ=255) tttagCGTGATTTTgtaggtcggataaggcgtttatgccgca < 1:1970746‑M2/41‑29 (MQ=255) tttagCGTGATTTTgtaggtcggataaggcgtttatgccgca < 1:1964225‑M2/41‑29 (MQ=255) tttagCGTGATTTTgtaggtcggataaggcgtttatgccg < 1:2549137‑M2/39‑27 (MQ=255) tttagCGTGATTTTgtaggtcggataaggcgtttatgcc > 1:3192519‑M2/2‑14 (MQ=255) tttagCGTGATTTTgtaggtcggataaggcgtttatgcc > 1:3576888‑M2/2‑14 (MQ=255) aTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCt > 1:3457347/1‑66 (MQ=255) aTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCt > 1:3459319/1‑66 (MQ=255) ttGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAAt > 1:815692/1‑62 (MQ=255) tGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATcc > 1:3887064/1‑42 (MQ=38) gCGTGATTTTgtaggtcggataaggcgtttacgccgcatccgacat < 1:4143606‑M2/46‑37 (MQ=255) gCGTGATTTTgtaggtcggataaggcgtttacgccgcatccgaca < 1:4111864‑M2/45‑36 (MQ=255) | CGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGA > Exported/2294010‑2294136 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |