Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 311,706 C→G 10.2% S389S (TCG→TCC acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported311,7060CG10.2% 376.9 / 23.6 127S389S (TCG→TCCacrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (60/54);  new base G (7/6);  total (67/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 128 total aligned reads displayed.

CCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATC  >  Exported/311636‑311775
                                                                      |                                                                     
ccACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATc                                                                       >  1:1276196/1‑71 (MQ=255)
  acaACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCga                                                                     <  1:2423164/71‑1 (MQ=255)
         tGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa                                                              <  1:1071670/71‑1 (MQ=255)
          ggCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa                                                              >  1:958283/1‑70 (MQ=255)
          ggCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa                                                              >  1:2725324/1‑70 (MQ=255)
           gcatCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaa                                                             >  1:1607906/4‑70 (MQ=9)
           gCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa                                                              >  1:3622721/1‑69 (MQ=255)
             gTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg                                                          <  1:2435204/71‑1 (MQ=255)
             gTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg                                                          >  1:5049028/1‑71 (MQ=255)
             gTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg                                                          >  1:5396240/1‑71 (MQ=255)
              tCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggc                                                         <  1:4854868/71‑1 (MQ=255)
              tCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggc                                                         <  1:1420939/71‑1 (MQ=255)
                aTCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcgg                                                       >  1:539611/1‑71 (MQ=255)
                 tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaa                                                             <  1:5550556/64‑1 (MQ=255)
                 tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaa                                                             <  1:2642586/64‑1 (MQ=255)
                 tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcgg                                                       >  1:4525407/1‑70 (MQ=255)
                 tCCACCAACAGGCAGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaa                                                             <  1:4851666/64‑1 (MQ=255)
                   caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc                                                      >  1:65230/1‑69 (MQ=255)
                   caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAg                                                           >  1:2174257/1‑64 (MQ=255)
                    accaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg                                                   <  1:2964428/71‑1 (MQ=255)
                    accaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg                                                   <  1:3421285/71‑1 (MQ=255)
                    accaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg                                                   <  1:3755918/71‑1 (MQ=255)
                      gagcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAagc                                                         <  1:600496/60‑1 (MQ=9)
                      gagcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGa                                                 <  1:3877372/68‑1 (MQ=12)
                      caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCga                                                                     <  1:510460/51‑1 (MQ=255)
                      caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa                                                 >  1:2970675/1‑71 (MQ=255)
                       aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGc                                                                >  1:2718452/1‑55 (MQ=255)
                       aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaaa                                                            >  1:793641/1‑59 (MQ=255)
                       aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa                                                              >  1:560383/1‑57 (MQ=255)
                       aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa                                                              >  1:5318445/1‑57 (MQ=255)
                       aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGAc                                                >  1:5483622/1‑71 (MQ=255)
                       aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGAc                                                >  1:469348/1‑71 (MQ=255)
                       aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGAc                                                >  1:3244469/1‑71 (MQ=255)
                        aCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg                                                   <  1:5028315/67‑1 (MQ=255)
                           ggCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa                                            <  1:4191323/71‑1 (MQ=255)
                           ggCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa                                            <  1:572928/71‑1 (MQ=255)
                            cccGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCaa                                           <  1:1437865/70‑1 (MQ=16)
                              cGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg                                         <  1:1094316/71‑1 (MQ=255)
                               gATGGCTAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGcc                                                               <  1:3399714/48‑1 (MQ=255)
                               gATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGcc                                                               <  1:3037034/48‑1 (MQ=255)
                               gATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGcc                                                               <  1:82209/48‑1 (MQ=255)
                               gATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGcc                                                               <  1:5422774/48‑1 (MQ=255)
                                aTGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc                                                      <  1:219989/56‑1 (MQ=255)
                                 tGGCGAGCACCATCCCTAACATTGTTATCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa                                            <  1:4981848/65‑1 (MQ=255)
                                 tGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaa                                                             <  1:1394458/48‑1 (MQ=255)
                                  ggCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgaga                                                                   >  1:37014/1‑41 (MQ=255)
                                  ggCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgaga                                                                   >  1:4550352/1‑41 (MQ=255)
                                   gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc                                    <  1:3276581/71‑1 (MQ=255)
                                   gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc                                    <  1:1865527/71‑1 (MQ=255)
                                   gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc                                    <  1:1676517/71‑1 (MQ=255)
                                   gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc                                    <  1:4340837/71‑1 (MQ=255)
                                     aaGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTccct                                   >  1:85032/2‑69 (MQ=12)
                                        cACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc                                >  1:1881803/1‑70 (MQ=255)
                                         aCCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa                                                 >  1:68116/1‑52 (MQ=255)
                                         aCCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa                                                 >  1:785974/1‑52 (MQ=255)
                                         aCCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTCCCTAACaa                              <  1:1302844/71‑1 (MQ=12)
                                          ccATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCa                               >  1:2964362/1‑69 (MQ=255)
                                          ccATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCa                               >  1:2161714/1‑69 (MQ=255)
                                          ccATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAAt                             <  1:4201267/71‑1 (MQ=255)
                                          ccATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTCCCTAACaac                             <  1:1962639/71‑2 (MQ=12)
                                           cATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc                                <  1:1733292/67‑1 (MQ=255)
                                           cATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTc                                      >  1:128890/1‑61 (MQ=12)
                                           cATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTCCCTAACaaca                            >  1:4166017/1‑69 (MQ=12)
                                             tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcgg                                                       >  1:3876650/1‑42 (MQ=255)
                                             tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa                                           <  1:4952290/54‑1 (MQ=255)
                                             tCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTCCCTAACaaca                            >  1:91188/1‑67 (MQ=12)
                                               ccGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc                                <  1:4266106/63‑1 (MQ=255)
                                                 gAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCg                      >  1:4877978/1‑71 (MQ=255)
                                                  aaCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc                                <  1:2224202/60‑1 (MQ=255)
                                                  aaCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg                     <  1:1693640/71‑1 (MQ=255)
                                                   aCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg                     >  1:4653480/1‑70 (MQ=255)
                                                    cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa                                                 >  1:3814848/1‑41 (MQ=255)
                                                    cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa                                            <  1:1352518/46‑1 (MQ=255)
                                                    cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCg                      >  1:1447142/1‑68 (MQ=255)
                                                    cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGAGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCg                      >  1:1376820/1‑68 (MQ=255)
                                                     aTTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTAc                  <  1:4654934/71‑1 (MQ=255)
                                                        gTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc                                    >  1:1032302/1‑50 (MQ=255)
                                                        gTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc                                    >  1:4456264/1‑50 (MQ=255)
                                                        gTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc                                    >  1:727980/1‑50 (MQ=255)
                                                        gTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc                                    >  1:1202656/1‑50 (MQ=255)
                                                         ttAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa                                                 <  1:4969280/36‑1 (MQ=255)
                                                          tAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCaa             >  1:196454/1‑71 (MQ=255)
                                                             cGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa                                           <  1:4855722/38‑1 (MQ=255)
                                                             cGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa                                           <  1:800495/38‑1 (MQ=255)
                                                              gtgtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg                                         >  1:4259160/1‑39 (MQ=255)
                                                              gtgtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg                                         >  1:5462723/1‑39 (MQ=255)
                                                              gtgtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg                                         >  1:2100296/1‑39 (MQ=255)
                                                               tgtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc                                <  1:1681563/47‑1 (MQ=255)
                                                                gtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgt                     <  1:4398025/57‑2 (MQ=255)
                                                                gtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg                     <  1:2718617/57‑1 (MQ=255)
                                                                gtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg                     <  1:3540056/57‑1 (MQ=255)
                                                                gtTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCg       >  1:3594483/1‑71 (MQ=255)
                                                                 tttATCGAGAAGCCAAAGGCGGCAAGGACGGCaaa                                          >  1:1771258/1‑35 (MQ=255)
                                                                 tttATCGAGAAGCCAAAGGCGGCAAGGACGGCaaa                                          >  1:2446163/1‑35 (MQ=255)
                                                                 tttATCGAGAAGCCAAAGGCGGCAAGGACGGCaaa                                          >  1:1336855/1‑35 (MQ=255)
                                                                 tttATCGAGAAGCCAAAGGCGGCAAGGACGGCaaa                                          >  1:2547344/1‑35 (MQ=255)
                                                                 tttATCGAGAAGCCAAAGGCGGCAAGGACGGCaaa                                          >  1:5392864/1‑35 (MQ=255)
                                                                 tttATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCgg      <  1:494426/71‑1 (MQ=255)
                                                                   tATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc                                    <  1:4050109/39‑1 (MQ=255)
                                                                    aTCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAAt   >  1:2996679/1‑71 (MQ=255)
                                                                      |                                                                     
CCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATC  >  Exported/311636‑311775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: