Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,374,528 A→T 26.8% C178S (TGC→AGC)  tatD ← DNase, magnesium‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,374,5280AT26.8% 88.6 / 38.2 56C178S (TGC→AGC) tatDDNase, magnesium‑dependent
Reads supporting (aligned to +/- strand):  ref base A (21/20);  new base T (6/9);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 5.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

GATCAGTAATTTTTCCGCCGGAATCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCCACGCACGCCTGCATCTCTTCGCGTGTG  >  Exported/2374460‑2374592
                                                                    |                                                                
gATCAGTAATTTTTCCGCCGGAATCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCaa                                                                 <  1:2829695/70‑1 (MQ=255)
        aTTTTTCCGCCGGAATCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCa                                                            <  1:892568/67‑1 (MQ=255)
        aTTTTTCCGCCGGAATCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCg                                                        >  1:459562/1‑71 (MQ=255)
        aTTTTTCCGCCGGAATCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCg                                                        >  1:2122918/1‑71 (MQ=255)
          ttttCCGCCGGAATCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCgg                                                       >  1:4014855/1‑70 (MQ=255)
           tttCCGCCGGAATCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCaa                                                           >  1:4789500/1‑65 (MQ=255)
           tttCCGCCGGAATCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCaa                                                           >  1:337177/1‑65 (MQ=255)
                gccgGAATCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGcc                                                <  1:1942003/71‑1 (MQ=255)
                  cgGAATCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCaa                                                                 <  1:216411/52‑1 (MQ=255)
                     aaTCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAAcc                                                         >  1:4629738/1‑57 (MQ=255)
                     aaTCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGTaa                                                    >  1:5239245/1‑62 (MQ=255)
                     aaTCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGTAAAGCCGata                                            >  1:1593150/1‑70 (MQ=255)
                       tCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCgg                                                       <  1:4400274/57‑1 (MQ=255)
                         aaCGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAAc                                                               >  1:375258/1‑47 (MQ=255)
                         aaCGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAAc                                                               >  1:832681/1‑47 (MQ=255)
                          aCGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAAtt                                      <  1:1523984/71‑1 (MQ=255)
                           cGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAATTc                                     <  1:599344/71‑1 (MQ=255)
                                      cccGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCcacg                          >  1:4840939/1‑71 (MQ=255)
                                      cccGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCcacg                          >  1:5587776/1‑71 (MQ=255)
                                       ccGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCcacgc                         <  1:4234168/71‑1 (MQ=255)
                                       ccGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCcacg                          >  1:1948260/1‑70 (MQ=255)
                                        cGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCcacgca                        >  1:2726293/1‑71 (MQ=255)
                                         gCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCcacgc                         >  1:560556/1‑69 (MQ=255)
                                         actgcgtcttttactggctcttcTCGCTAACCCaa                                                           <  1:5464082‑M1/12‑1 (MQ=255)
                                         actgcgtcttttactggctcttcTCGCTAACCCaa                                                           <  1:3815422‑M1/12‑1 (MQ=255)
                                         actgcgtcttttactggctcttcTCGCTAACCCAAc                                                          <  1:4304800‑M1/13‑1 (MQ=255)
                                         actgcgtcttttactggctcttcTCGCTAACCCAAc                                                          <  1:5605238‑M1/13‑1 (MQ=255)
                                         actgcgtcttttactggctcttcTCGCTAACCCAACCg                                                        <  1:296584‑M1/15‑1 (MQ=255)
                                         actgcgtcttttactggctcttcTCGCTAACCCAACCg                                                        <  1:3476691‑M1/15‑1 (MQ=255)
                                         actgcgtcttttactggctcttcTCGCTAACCCAACCGGt                                                      >  1:311424‑M1/24‑40 (MQ=255)
                                         actgcgtcttttactggctcttcTCGCTAACCCAACCGGt                                                      >  1:5203469‑M1/24‑40 (MQ=255)
                                         actgcgtcttttactggctcttcTCGCTAACCCAACCGGt                                                      >  1:4638053‑M1/24‑40 (MQ=255)
                                         actgcgtcttttactggctcttcTCGCTAACCCAACCGGt                                                      >  1:221378‑M1/24‑40 (MQ=255)
                                         actgcgtcttttactggctcttcTCGCTAACCCAACCGGTa                                                     <  1:315000‑M1/18‑1 (MQ=255)
                                         actgcgtcttttactggctcttcTCGCTAAACCAACCGGTa                                                     <  1:4281735‑M1/18‑1 (MQ=255)
                                           tgcgtcttttactggctcttcTCGCTAACCCAACCGGt                                                      >  1:1569305‑M1/22‑38 (MQ=255)
                                            gcgtcttttactggctcttcTCGCTAACCCAACCGGt                                                      >  1:181526‑M1/21‑37 (MQ=255)
                                            gCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGt                                                      <  1:2417244/37‑1 (MQ=255)
                                            gCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGt                                                      <  1:2252053/37‑1 (MQ=255)
                                               tcttttactggctcttcTCGCTAACCCAACCGGTaa                                                    <  1:4290858‑M1/19‑1 (MQ=255)
                                               ccAGTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGCCGATATaaa                                        >  1:5040273/1‑48 (MQ=255)
                                                cAGTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCCACGCACGCCTGc                 <  1:4269696/70‑1 (MQ=255)
                                                  gTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAAt                                       <  1:4933495/46‑1 (MQ=255)
                                                  gTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAATTc                                     <  1:1785000/48‑1 (MQ=255)
                                                     cgcgTCGTTCATCGCAAACCCAACCGGTAATGCCGATATaa                                         >  1:616850/1‑41 (MQ=255)
                                                     cgcgTCGTTCATCGCAAACCCAACCGGTAATGCCGATATaa                                         >  1:1964198/1‑41 (MQ=255)
                                                     cgcgTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCAt                                  >  1:3188071/1‑48 (MQ=255)
                                                     cgcgTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCCACGCACGCCTGCATctct           >  1:989722/1‑71 (MQ=255)
                                                     cgcgTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCCACGCACGCCTGCATctct           >  1:4817289/1‑71 (MQ=255)
                                                        gtcgtTCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCCACGCACGCCTGCATCTCTTc         <  1:1363117/70‑1 (MQ=255)
                                                            ttCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCCACGCACGCCTGCATctct           <  1:2836345/64‑1 (MQ=255)
                                                            ttCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCCACGCACGCCTGCATCTCTTCGCgt     <  1:5316504/70‑1 (MQ=255)
                                                               aTCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCCACGCACGCCTGCATCTCTTCGCGtgtg  <  1:2937724/70‑1 (MQ=255)
                                                                tCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCCACGCACGCCTGCATCTCTTcgc       <  1:774073/64‑1 (MQ=255)
                                                                 cGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCcacg                          <  1:4241340/44‑1 (MQ=255)
                                                                 cGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCcacg                          <  1:340252/44‑1 (MQ=255)
                                                                 cGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCcacg                          <  1:3248664/44‑1 (MQ=255)
                                                                 cGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCcacg                          <  1:825951/44‑1 (MQ=255)
                                                                    aaaCCCAACCGGTAATGCCGATATAAATTCCATGCGCCACGCACGcc                    >  1:4064237/1‑47 (MQ=255)
                                                                    |                                                                
GATCAGTAATTTTTCCGCCGGAATCAACGGCAAAAGTTCCCGCAGCTCCAGTCCGCGTCGTTCATCGCAAACCCAACCGGTAATGCCGATATAAATTCCATGCGCCACGCACGCCTGCATCTCTTCGCGTGTG  >  Exported/2374460‑2374592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: