BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,592	G→A	6.5%	T495T (ACC→ACT)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,592	0	G	A	6.5%	320.3 / 6.9	107	T495T (ACC→ACT)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base G (43/57); new base A (4/3); total (47/60)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

AGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTG  >  Exported/2562523‑2562661

aGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCgg                                                                      >  1:1643765/1‑71 (MQ=255)

  aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGt                                                                     <  1:3799749/70‑1 (MQ=255)

      tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                                                                >  1:5659472/1‑71 (MQ=255)

      tttGCCCACCAGGACTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                                                                >  1:4463872/1‑71 (MQ=255)

         gCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGccgc                                                             <  1:4157060/71‑3 (MQ=255)

         gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGa                                                                   >  1:3194293/1‑65 (MQ=255)

         gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                                                             >  1:1941283/1‑71 (MQ=255)

         gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                                                             >  1:5111582/1‑71 (MQ=255)

          cccACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                                                             <  1:2776999/70‑1 (MQ=255)

           ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg                                                           >  1:3093636/1‑71 (MQ=255)

               caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg                                                       <  1:1457428/71‑1 (MQ=255)

                aGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg                                                       >  1:3101389/1‑70 (MQ=255)

                 ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                                                     >  1:4464874/1‑71 (MQ=255)

                    cTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg                                                  >  1:4143537/1‑71 (MQ=255)

                      gtcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgc                                                >  1:4290080/3‑71 (MQ=255)

                      gtcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgc                                                >  1:2997310/3‑71 (MQ=255)

                       tcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgct                                               >  1:2850094/2‑70 (MQ=255)

                       ccgccgCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggc                                                <  1:1358432/70‑1 (MQ=255)

                        cgccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTgg                                                 <  1:4372768/68‑1 (MQ=255)

                         gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGt                                                                     <  1:2815257/47‑1 (MQ=255)

                         gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggcgg                                              >  1:4962567/1‑70 (MQ=255)

                            gcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc                                          <  1:905535/71‑1 (MQ=255)

                            gcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc                                          <  1:38867/71‑1 (MQ=255)

                              acaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc                                          >  1:4993910/1‑69 (MQ=255)

                               caGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                                                     >  1:3867121/1‑57 (MQ=255)

                               caGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                                                     >  1:1403883/1‑57 (MQ=255)

                                aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc                                      <  1:4477575/71‑1 (MQ=255)

                                aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc                                      <  1:5458937/71‑1 (MQ=255)

                                 ggCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggt                                                         >  1:1532170/1‑51 (MQ=255)

                                 ggCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc                                      >  1:1784924/1‑70 (MQ=255)

                                  gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc                                      <  1:3603062/69‑1 (MQ=255)

                                  gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                    <  1:4956448/71‑1 (MQ=255)

                                  gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                    >  1:3198310/1‑71 (MQ=255)

                                  gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                    >  1:3698900/1‑71 (MQ=255)

                                  gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                    >  1:3692022/1‑71 (MQ=255)

                                    gatagcgatagATTGCGGGGAGATCATCTTACCGGTGa                                                                   <  1:4402374/38‑1 (MQ=255)

                                    gatagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                    >  1:3006952/1‑69 (MQ=255)

                                      tagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                    >  1:760940/1‑67 (MQ=255)

                                      tagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGa                                <  1:4985427/71‑1 (MQ=255)

                                      tagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGa                                <  1:933193/71‑1 (MQ=255)

                                      cggcgatagATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTTGCTGCCAc                                          >  1:3575025/3‑61 (MQ=255)

                                       agcgatagATTGCGGGGAGATCATCTTACCGGTGa                                                                   >  1:322529/1‑35 (MQ=255)

                                       agcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtg                                                        >  1:4864617/1‑46 (MQ=255)

                                       agcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtg                                                        >  1:884592/1‑46 (MQ=255)

                                       agcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                    >  1:1040689/1‑66 (MQ=255)

                                       agcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                    >  1:2991359/1‑66 (MQ=255)

                                        gcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGAt                               <  1:3589432/70‑1 (MQ=255)

                                        gcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGAt                               <  1:3799621/70‑1 (MQ=255)

                                         cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATc                              <  1:4468703/70‑1 (MQ=255)

                                         cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                             <  1:2206542/71‑1 (MQ=255)

                                         cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                             <  1:732248/71‑1 (MQ=255)

                                         cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                             <  1:2402859/71‑1 (MQ=255)

                                         cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                             <  1:3975860/71‑1 (MQ=255)

                                           atagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCaga                           <  1:4903605/71‑1 (MQ=255)

                                           atagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                             <  1:4125777/69‑1 (MQ=255)

                                           atagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                             <  1:2099888/69‑1 (MQ=255)

                                            tagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                             >  1:3856092/1‑68 (MQ=255)

                                             agATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCagag                          >  1:5168142/1‑70 (MQ=255)

                                             agATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAgaga                         >  1:4826657/1‑71 (MQ=255)

                                             agATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAgaga                         >  1:3199942/1‑71 (MQ=255)

                                              gATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAgaga                         >  1:4528684/1‑70 (MQ=255)

                                               aTTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTTGCTGCCACCAGCa                                     <  1:4719050/57‑1 (MQ=255)

                                               aTTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                             <  1:4274733/65‑1 (MQ=255)

                                               aTTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                             <  1:521037/65‑1 (MQ=255)

                                               aTTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                             <  1:466454/65‑1 (MQ=255)

                                               aTTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACg                       >  1:2290931/1‑71 (MQ=255)

                                                      ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt                >  1:3305907/1‑71 (MQ=255)

                                                      ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt                <  1:5347637/71‑1 (MQ=255)

                                                      ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt                <  1:1706535/71‑1 (MQ=255)

                                                      ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt                <  1:2742185/71‑1 (MQ=255)

                                                      ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt                <  1:2518998/71‑1 (MQ=255)

                                                      ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACAAGCAACAGATCAGAGACGCCAAttt                <  1:4530105/71‑1 (MQ=255)

                                                       gagaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttg               <  1:5386954/71‑1 (MQ=255)

                                                       gagaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttg               <  1:5653578/71‑1 (MQ=255)

                                                       gagaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttg               <  1:725154/71‑1 (MQ=255)

                                                        agaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgt              <  1:3876918/71‑1 (MQ=255)

                                                        agaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgt              <  1:1250462/71‑1 (MQ=255)

                                                          atcatcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGcc                     >  1:1369123/1‑62 (MQ=255)

                                                          atcatcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGc                      <  1:5144430/61‑1 (MQ=255)

                                                           tcatcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACg                       <  1:1601814/59‑1 (MQ=255)

                                                           tcatcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACg                       <  1:1897474/59‑1 (MQ=255)

                                                            catcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgttg            <  1:2369473/69‑1 (MQ=255)

                                                            catcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTtgttgt           >  1:5348102/1‑70 (MQ=255)

                                                            catcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTtgtg          >  1:1459403/1‑71 (MQ=255)

                                                              tcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt                <  1:5438080/63‑1 (MQ=255)

                                                               cTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgttg            >  1:4879143/1‑66 (MQ=255)

                                                               cTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgc         >  1:2737973/1‑69 (MQ=255)

                                                                ttACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgctgc      >  1:411249/1‑71 (MQ=255)

                                                                 tACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttg               >  1:3090777/1‑61 (MQ=255)

                                                                  aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgctgc      <  1:2011742/69‑1 (MQ=255)

                                                                  aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgctgc      <  1:514004/69‑1 (MQ=255)

                                                                  aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgct        <  1:2268682/67‑1 (MQ=255)

                                                                  aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgct        <  1:2085545/67‑1 (MQ=255)

                                                                  aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCgg    >  1:1921218/1‑71 (MQ=255)

                                                                  aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCg     <  1:2193849/70‑1 (MQ=255)

                                                                    cGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgttg            <  1:4991421/61‑1 (MQ=255)

                                                                    cGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgttg            <  1:2623471/61‑1 (MQ=255)

                                                                    cGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgttg            <  1:4908609/61‑1 (MQ=255)

                                                                    cGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgttg            <  1:1003998/61‑1 (MQ=255)

                                                                    cGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTg  <  1:4458571/71‑1 (MQ=255)

AGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTG  >  Exported/2562523‑2562661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`