BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,798	G→T	7.0%	L427M (CTG→ATG)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,798	0	G	T	7.0%	368.7 / 7.4	114	L427M (CTG→ATG)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base G (59/47); new base T (3/5); total (62/52)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 117 total aligned reads displayed.

CGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAG  >  Exported/2562729‑2562864

cGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGt                                                                   >  1:1914376/1‑71 (MQ=255)

 gAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTc                                                                  >  1:4456498/1‑71 (MQ=255)

    gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCGAGTGTTGATGACAGTCCg                                                                >  1:1519842/1‑70 (MQ=255)

    gAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCgg                                                               <  1:5551658/71‑1 (MQ=255)

      aCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGaa                                                             >  1:3912729/1‑71 (MQ=255)

      aCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGaa                                                             <  1:1743839/71‑1 (MQ=255)

      aCGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGaa                                                             <  1:1619930/71‑1 (MQ=255)

           gagaAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGtt                                                        >  1:5157284/1‑71 (MQ=255)

            agaagaAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTc                                                       <  1:3103375/71‑1 (MQ=255)

              aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTcc                                                                 <  1:618363/59‑1 (MQ=255)

              aagaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa                                                     <  1:1586393/71‑1 (MQ=255)

               agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg                                                      >  1:4599888/1‑69 (MQ=255)

               agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg                                                      >  1:5578002/1‑69 (MQ=255)

               agaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa                                                     >  1:4212015/1‑70 (MQ=255)

                gaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                                   >  1:3084253/1‑71 (MQ=255)

                gaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                                   >  1:290423/1‑71 (MQ=255)

                  agGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAt                                                  <  1:5508246/70‑1 (MQ=255)

                        aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                           <  1:5047878/71‑1 (MQ=255)

                        aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                           <  1:835765/71‑1 (MQ=255)

                        aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                           <  1:3844628/71‑1 (MQ=255)

                        aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                           <  1:5415613/71‑1 (MQ=255)

                        aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                           <  1:2842602/71‑1 (MQ=255)

                        aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                           <  1:2597269/71‑1 (MQ=255)

                        aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg                                           <  1:1409973/71‑1 (MQ=255)

                          gCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATaa                                                <  1:2481695/64‑1 (MQ=255)

                           cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                        >  1:106148/1‑71 (MQ=255)

                           cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                        >  1:1106407/1‑71 (MQ=255)

                           cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                        >  1:3893123/1‑71 (MQ=255)

                           cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt                                        >  1:5169307/1‑71 (MQ=255)

                           cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCAAAATAAAGGCGAAt                                        >  1:638628/1‑71 (MQ=255)

                               aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaa                                                    >  1:5344643/1‑55 (MQ=255)

                               aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAg                                              >  1:3647968/1‑61 (MQ=255)

                               aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGc                                      >  1:4808018/1‑69 (MQ=255)

                                ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGc                                            >  1:5466577/1‑62 (MQ=255)

                                ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAg                                   >  1:1020275/1‑71 (MQ=255)

                                ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAg                                   <  1:4606298/71‑1 (MQ=255)

                                 cGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                 <  1:4622033/56‑1 (MQ=255)

                                 cGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                 <  1:5176153/56‑1 (MQ=255)

                                    tgtgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCg                                                      <  1:1141503/48‑1 (MQ=255)

                                      tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa                                                     <  1:5610371/47‑1 (MQ=255)

                                      tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGa                                                     <  1:1259051/47‑1 (MQ=255)

                                      tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATaaa                                               <  1:4947671/53‑1 (MQ=255)

                                        cgcCAGTGCCAGCGCCAGTGTTGATGCCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc                           >  1:4262622/1‑71 (MQ=255)

                                        cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                                   >  1:4698748/1‑47 (MQ=255)

                                        cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                                   >  1:1587077/1‑47 (MQ=255)

                                        cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                                   >  1:1418383/1‑47 (MQ=255)

                                        cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa                                                   >  1:2550696/1‑47 (MQ=255)

                                        cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAt                                                  <  1:5028110/48‑1 (MQ=255)

                                        cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc                           >  1:5556386/1‑71 (MQ=255)

                                          ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAcc                          >  1:4946521/1‑70 (MQ=255)

                                          ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAcc                          >  1:3398620/1‑70 (MQ=255)

                                          ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCg                         <  1:4700014/71‑1 (MQ=255)

                                           cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCa                              <  1:2633657/65‑1 (MQ=255)

                                           cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGccaa                        <  1:5280274/71‑3 (MQ=255)

                                           cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGccaa                        <  1:4206624/71‑3 (MQ=255)

                                            aGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                       >  1:239227/1‑71 (MQ=255)

                                            aGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                       >  1:2159907/1‑71 (MQ=255)

                                              tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                 <  1:4429701/43‑1 (MQ=255)

                                              tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                 <  1:1021423/43‑1 (MQ=255)

                                              tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAgg                                             <  1:4159643/47‑1 (MQ=255)

                                              tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAgg                                             <  1:3974195/47‑1 (MQ=255)

                                                ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                    <  1:408708/70‑1 (MQ=255)

                                                ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                    >  1:118170/1‑70 (MQ=255)

                                                ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                    >  1:769714/1‑70 (MQ=255)

                                                ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATa                            >  1:1750329/1‑62 (MQ=255)

                                                ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                   >  1:1110052/1‑71 (MQ=255)

                                                 cAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                   >  1:2693699/1‑70 (MQ=255)

                                                 cAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                   >  1:5599599/1‑70 (MQ=255)

                                                 cAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                    <  1:2241158/69‑1 (MQ=255)

                                                 cAGCGACAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATCCCGATGGAg                   >  1:2008963/1‑70 (MQ=255)

                                                  aGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa                                                 <  1:1635298/39‑1 (MQ=255)

                                                    cgcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg                   >  1:4506506/1‑67 (MQ=255)

                                                     gcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc                           <  1:4140878/58‑1 (MQ=255)

                                                     gcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa               >  1:4009300/1‑70 (MQ=255)

                                                      ccAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa                 <  1:5275838/67‑1 (MQ=255)

                                                        aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCa                              >  1:3025819/1‑52 (MQ=255)

                                                        aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg           <  1:3210737/71‑1 (MQ=255)

                                                         gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa                                         >  1:2072745/1‑40 (MQ=255)

                                                         gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa                                         >  1:5662538/1‑40 (MQ=255)

                                                         gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa                                         >  1:1405628/1‑40 (MQ=255)

                                                         gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGa                                          <  1:2713171/39‑1 (MQ=255)

                                                         gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg           >  1:4043806/1‑70 (MQ=255)

                                                          tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt                       <  1:464769/57‑1 (MQ=255)

                                                          tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa         <  1:1048842/71‑1 (MQ=255)

                                                          tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa         <  1:5484630/71‑1 (MQ=255)

                                                           gtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCgtt                       <  1:33239/56‑3 (MQ=255)

                                                            ttGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATgg                     >  1:4503950/1‑57 (MQ=255)

                                                             tgatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCaga       <  1:2691275/70‑1 (MQ=255)

                                                              gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa                                         >  1:4942369/1‑35 (MQ=255)

                                                              gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa                                         >  1:1249381/1‑35 (MQ=255)

                                                              gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa                                         >  1:4512518/1‑35 (MQ=255)

                                                              gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc     >  1:5530277/1‑71 (MQ=255)

                                                              gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc     >  1:3373119/1‑71 (MQ=255)

                                                              gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc     >  1:741882/1‑71 (MQ=255)

                                                              gatgaCAGT‑CGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc     >  1:3243766/1‑70 (MQ=255)

                                                                 gaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCAt                             >  1:3298977/1‑44 (MQ=255)

                                                                   cAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCag        >  1:1691005/1‑63 (MQ=255)

                                                                     gTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa               >  1:1311283/1‑54 (MQ=255)

                                                                     gTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa               >  1:1273389/1‑54 (MQ=255)

                                                                     gTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCACACCGATGGAGTAGa               >  1:5237428/1‑54 (MQ=255)

CGAGGAACGGCGAGAAGAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAG  >  Exported/2562729‑2562864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`