| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,562,814 | A→G | 5.7% | I421I (ATT→ATC) ‡ | lldP ← | L‑lactate permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,562,814 | 0 | A | G | 5.7% | 401.9 / 9.8 | 139 | I421I (ATT→ATC) ‡ | lldP | L‑lactate permease |
| Reads supporting (aligned to +/- strand): ref base A (69/62); new base G (3/5); total (72/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 | |||||||||||
Only 100 of 152 total aligned reads displayed.
GAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCTGCCGA > Exported/2562745‑2562884 | gaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa > 1:290423/1‑71 (MQ=255)gaagGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa > 1:3084253/1‑71 (MQ=255) aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg < 1:2822310/71‑1 (MQ=255) aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg < 1:5047878/71‑1 (MQ=255) aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg < 1:3844628/71‑1 (MQ=255) aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg < 1:835765/71‑1 (MQ=255) aTGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCg < 1:1409973/71‑1 (MQ=255) gCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATaa < 1:2481695/64‑1 (MQ=255) cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt > 1:5169307/1‑71 (MQ=255) cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt > 1:3893123/1‑71 (MQ=255) cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAAt > 1:106148/1‑71 (MQ=255) cATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCAAAATAAAGGCGAAt > 1:638628/1‑71 (MQ=255) aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaa > 1:5344643/1‑55 (MQ=255) aCCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAg > 1:3647968/1‑61 (MQ=255) ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGc > 1:5466577/1‑62 (MQ=255) ccGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAg > 1:1020275/1‑71 (MQ=255) cGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa < 1:4622033/56‑1 (MQ=255) cGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa < 1:5176153/56‑1 (MQ=255) tgCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATaaa < 1:4947671/53‑1 (MQ=255) cgcCAGTGCCAGCGCCAGTGTTGATGCCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc > 1:4262622/1‑71 (MQ=255) cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa > 1:1587077/1‑47 (MQ=255) cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa > 1:2550696/1‑47 (MQ=255) cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGaaa > 1:1418383/1‑47 (MQ=255) cgcCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAt < 1:5028110/48‑1 (MQ=255) ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAcc > 1:3398620/1‑70 (MQ=255) ccAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCg < 1:4700014/71‑1 (MQ=255) cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCa < 1:2633657/65‑1 (MQ=255) cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGccaa < 1:4206624/71‑3 (MQ=255) cAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGccaa < 1:5280274/71‑3 (MQ=255) aGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt > 1:239227/1‑71 (MQ=255) gTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATg < 1:1936672/71‑1 (MQ=255) tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa < 1:1021423/43‑1 (MQ=255) tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATa < 1:3836005/43‑1 (MQ=255) tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAgg < 1:3974195/47‑1 (MQ=255) tGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAAT‑AAGGCGAATGCCAGCACCATACCGATGGa < 1:1114507/71‑1 (MQ=255) accagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa > 1:4741748/2‑71 (MQ=255) ccagcgccagcgTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa > 1:769714/1‑70 (MQ=255) ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATa > 1:1750329/1‑62 (MQ=255) ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg > 1:5545325/1‑71 (MQ=255) ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg < 1:3234846/71‑1 (MQ=255) ccAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg > 1:1110052/1‑71 (MQ=255) cAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg > 1:5599599/1‑70 (MQ=255) cAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa < 1:2241158/69‑1 (MQ=255) cgcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAg > 1:4506506/1‑67 (MQ=255) gcCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATAc < 1:4140878/58‑1 (MQ=255) ccAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTa < 1:5275838/67‑1 (MQ=255) aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCa > 1:3025819/1‑52 (MQ=255) aGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCa > 1:1596421/1‑52 (MQ=255) gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa > 1:5662538/1‑40 (MQ=255) gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGaa > 1:2072745/1‑40 (MQ=255) gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGa < 1:2713171/39‑1 (MQ=255) gtgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATggg > 1:4043806/1‑70 (MQ=255) tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt < 1:464769/57‑1 (MQ=255) tgtTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa < 1:5484630/71‑1 (MQ=255) tgatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCaga < 1:2691275/70‑1 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa > 1:4021223/1‑35 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa > 1:4942369/1‑35 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa > 1:1249381/1‑35 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGaa > 1:4512518/1‑35 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc > 1:3373119/1‑71 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc > 1:1380088/1‑71 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc > 1:741882/1‑71 (MQ=255) gatgaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc > 1:5530277/1‑71 (MQ=255) gatgaCAGT‑CGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc > 1:3243766/1‑70 (MQ=255) gaCAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCAt > 1:3298977/1‑44 (MQ=255) cAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCag > 1:1691005/1‑63 (MQ=255) gTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGa > 1:1311283/1‑54 (MQ=255) gTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCACACCGATGGAGTAGa > 1:5237428/1‑54 (MQ=255) tCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAg < 1:1224405/52‑1 (MQ=255) cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCagag > 1:895726/1‑60 (MQ=255) cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAg > 1:4927924/1‑64 (MQ=255) cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCttt < 1:2064984/70‑1 (MQ=255) cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCttt > 1:4434644/1‑70 (MQ=255) cGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGAT‑‑GCAGAGCCAGTTCTTTCAg > 1:3351507/1‑71 (MQ=255) gAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCg < 1:1667538/39‑1 (MQ=255) gAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAg < 1:1354016/71‑1 (MQ=255) gAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAg < 1:4092279/71‑1 (MQ=255) aaTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGc > 1:98151/1‑71 (MQ=255) aTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCg > 1:2293695/1‑71 (MQ=255) aTAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCg > 1:4474416/1‑71 (MQ=255) tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGa < 1:4163940/37‑1 (MQ=255) tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt < 1:5232841/38‑1 (MQ=255) tAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGAt < 1:2100207/38‑1 (MQ=255) tAGTTCGAAATAAACGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGc < 1:5394239/69‑1 (MQ=255) aGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCa < 1:3090354/51‑1 (MQ=255) gTTCGAAATAAAGGCGAATGCCAGCACCATACCGATg > 1:636022/1‑37 (MQ=255) gTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGc > 1:1397448/1‑54 (MQ=255) gTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtgc < 1:230483/71‑1 (MQ=255) gTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtgc > 1:856961/1‑71 (MQ=255) gTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtgc > 1:3021505/1‑71 (MQ=255) ttCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGtt < 1:89909/58‑1 (MQ=255) ttCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtgct < 1:1887200/71‑1 (MQ=255) ttCGAAATAAAGGCGAATGCCAGCACCATACCGAAGGAGTAGATGGGCAGAGCCAGtt < 1:1658827/58‑1 (MQ=255) cGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCa > 1:4261842/1‑53 (MQ=255) cGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGtg > 1:2850442/1‑67 (MQ=255) gAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCTGcc < 1:2541147/71‑1 (MQ=255) aaaTAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGt < 1:2167776/53‑1 (MQ=255) aaaTAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGt < 1:3644682/53‑1 (MQ=255) aaaTAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGt < 1:1054866/53‑1 (MQ=255) aaTAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCTGCCGa < 1:3264120/71‑1 (MQ=255) | GAAGGTGAATGCATGACCGGTGTGCGCCAGTGCCAGCGCCAGTGTTGATGACAGTCCGGAATAGTTCGAAATAAAGGCGAATGCCAGCACCATACCGATGGAGTAGATGGGCAGAGCCAGTTCTTTCAGCGTGCTGCCGA > Exported/2562745‑2562884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |