Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,101 T→A 7.8% I32F (ATC→TTC) ‡ mtlA ← fused mannitol‑specific PTS enzyme IIABC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,1010TA 7.8% 171.3 / 6.2 64I32F (ATC→TTC) ‡mtlAfused mannitol‑specific PTS enzyme IIABC components
Reads supporting (aligned to +/- strand):  ref base T (30/29);  new base A (3/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

TCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTGCACT  >  Exported/2569032‑2569171
                                                                     |                                                                      
tcGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATa                                                                       <  1:2056632/71‑1 (MQ=255)
        aCCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCAcgc                                                               <  1:3845802/71‑1 (MQ=255)
        aCCAGCTTCCCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCAcgc                                                               <  1:1166793/71‑1 (MQ=255)
         ccAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCAcgc                                                               >  1:4679329/1‑70 (MQ=255)
          cAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGa                                                             >  1:67731/1‑71 (MQ=255)
           aGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGAt                                                            <  1:5698130/71‑1 (MQ=255)
             cTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATa                                                           >  1:2675980/1‑70 (MQ=255)
              ttCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATAccc                                                                    >  1:4633505/1‑60 (MQ=255)
              ttCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATAccc                                                                    >  1:648603/1‑60 (MQ=255)
                cGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAAcg                                                       >  1:2617703/1‑71 (MQ=255)
                cGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAAAAAATAACGCTGTGATGATACGCCACGCGATa                                                           <  1:29603/67‑1 (MQ=255)
                 gCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAAcgc                                                      <  1:2425969/71‑1 (MQ=255)
                    aGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGcc                                                   >  1:4570591/1‑71 (MQ=255)
                    aGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGcc                                                   >  1:5613050/1‑71 (MQ=255)
                        tctcGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGata                                               <  1:5634626/71‑1 (MQ=255)
                         ttcGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAata                                               >  1:2891923/2‑70 (MQ=255)
                           cGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATAttt                                            >  1:4618268/1‑71 (MQ=255)
                           cGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATAtt                                             <  1:2015882/70‑1 (MQ=255)
                            gTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATAttt                                            <  1:517938/70‑1 (MQ=255)
                             ttCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGatat                                              <  1:4230686/67‑1 (MQ=255)
                               cGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGc                                         <  1:4888212/70‑1 (MQ=255)
                                    gccaacCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGAtg                                      <  1:2863261/67‑2 (MQ=255)
                                     ccaacCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCa                                  >  1:4707540/1‑71 (MQ=255)
                                      caccCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGata                                               >  1:255166/1‑57 (MQ=255)
                                      caccCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCAt                                 >  1:2490011/1‑71 (MQ=255)
                                      caccCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCAt                                 >  1:5082293/1‑71 (MQ=255)
                                      caccCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCAt                                 >  1:51359/1‑71 (MQ=255)
                                          cTGTTGGAATAAATAACGCGGTGATGATACCCCAcgc                                                               <  1:2227238/37‑1 (MQ=255)
                                          cTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTg                             >  1:4581898/1‑71 (MQ=255)
                                             ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGata                                               <  1:4769913/50‑1 (MQ=255)
                                             ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATAttt                                            <  1:2644061/53‑1 (MQ=255)
                                             ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATAttt                                            <  1:292667/53‑1 (MQ=255)
                                             ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATAttt                                            <  1:491361/53‑1 (MQ=255)
                                             ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCt                           <  1:24541/70‑1 (MQ=255)
                                             ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCt                           <  1:1896580/70‑1 (MQ=255)
                                             ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCt                           <  1:2837901/70‑1 (MQ=255)
                                             ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTg                          <  1:5613654/71‑1 (MQ=255)
                                             ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTg                          <  1:618069/71‑1 (MQ=255)
                                             ttGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTg                          <  1:2391542/71‑1 (MQ=255)
                                              tgtaataaataACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCAc                                    <  1:3122720/57‑1 (MQ=255)
                                              tGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCAc                                    <  1:3154253/60‑1 (MQ=255)
                                               ggAATAAATAACGCGGTGATGATACCCCACGCGATa                                                           >  1:3203967/1‑36 (MQ=255)
                                               ggAATAAATAACGCGGTGATGATACCCCACGCGATa                                                           >  1:2979551/1‑36 (MQ=255)
                                               ggAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGata                                               >  1:1594840/1‑48 (MQ=255)
                                               ggAATAAATAACGCAGTAATAAAACCCCAGGCAAAAAAAGCACCAata                                               >  1:781574/1‑48 (MQ=255)
                                                gAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGata                                               <  1:2249419/47‑1 (MQ=255)
                                                gAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGat                                                >  1:372050/1‑46 (MQ=255)
                                                 aataaataACGCGGTGATGATACCCCACGCGATAAACGCGACGATATTTGGCATCACCATGTTGCTGAgg                       >  1:22585/1‑70 (MQ=255)
                                                  ataaataaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGa                      >  1:3994007/1‑70 (MQ=255)
                                                   taaataaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATAttt                                            <  1:535203/47‑1 (MQ=255)
                                                    aaataaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAAc                   >  1:4707280/1‑71 (MQ=255)
                                                     aataaCGCGGTGATGATACCCCACGCGATAAACGCGCCGata                                               >  1:1675760/1‑42 (MQ=255)
                                                     aataaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTgg                                          >  1:5385183/1‑47 (MQ=255)
                                                     aataaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTg                                           >  1:3337096/1‑46 (MQ=255)
                                                       taaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCt                           <  1:3230517/60‑1 (MQ=255)
                                                       taaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGAc                >  1:502137/1‑71 (MQ=255)
                                                        aaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATAtt                                             >  1:4271999/1‑41 (MQ=255)
                                                        aaCGCGGTGATGATACCCCACGCGATAAACGCGCCGATAtt                                             >  1:5348717/1‑41 (MQ=255)
                                                         aCGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGa                         >  1:2991964/1‑60 (MQ=255)
                                                          cgcgGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCAt                                       <  1:5310353/45‑1 (MQ=255)
                                                          cgcgGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGAcc               >  1:1017178/1‑69 (MQ=255)
                                                            cgGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAg           <  1:139966/71‑1 (MQ=255)
                                                               tgatgaTACCCCACGCTATAAACGCGCCGATAttt                                            <  1:3177720/35‑1 (MQ=255)
                                                               tgatgaTACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCtt        >  1:2149476/1‑71 (MQ=255)
                                                                gatgatACCCCACGCGATAAACGAGCCGATATTTGGCATCACCATGTTGCTGAGGa                      <  1:2487797/56‑1 (MQ=255)
                                                                 atgatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTg                          <  1:231921/51‑1 (MQ=255)
                                                                 atgatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCttt       >  1:834186/1‑70 (MQ=255)
                                                                 atgatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTg      >  1:5212328/1‑71 (MQ=255)
                                                                 atgatACCACACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGaa                     <  1:5528332/56‑1 (MQ=255)
                                                                   gatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAAc                   >  1:3814279/1‑56 (MQ=255)
                                                                   gatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAAc                   >  1:2289835/1‑56 (MQ=255)
                                                                   gatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAAc                   >  1:2072208/1‑56 (MQ=255)
                                                                   gatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTGCa    >  1:4830166/1‑71 (MQ=255)
                                                                   gatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTGCa    >  1:3068868/1‑71 (MQ=255)
                                                                   gatACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTGCa    >  1:1368189/1‑71 (MQ=255)
                                                                    atACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCaaa            >  1:2450131/1‑62 (MQ=255)
                                                                    atACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAg           <  1:3611785/63‑1 (MQ=255)
                                                                     tACCCCACGCGATAAACGCGCCGATATTTGGCATc                                      <  1:2147013/35‑1 (MQ=255)
                                                                     tACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCt         <  1:2390696/64‑1 (MQ=255)
                                                                     tACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTGCACt  <  1:821188/71‑1 (MQ=255)
                                                                     tACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTGCACt  <  1:1097156/71‑1 (MQ=255)
                                                                     |                                                                      
TCGGCCCGACCAGCTTCGCCAGCGTCTCGTTCGGTAACCACCCTGTTGGAATAAATAACGCGGTGATGATACCCCACGCGATAAACGCGCCGATATTTGGCATCACCATGTTGCTGAGGAAACGACCAAAGCTTTGCACT  >  Exported/2569032‑2569171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: