Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,820 T→C 9.7% Y30Y (TAT→TAC yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,8200TC 9.7% 456.6 / 28.2 168Y30Y (TAT→TACyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (79/72);  new base C (9/7);  total (89/79)
Fisher's exact test for biased strand distribution p-value = 7.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 170 total aligned reads displayed.

GTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTT  >  Exported/2569751‑2569888
                                                                     |                                                                    
gTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATc                                                                     >  1:1804155/1‑71 (MQ=255)
 ttGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                                    <  1:2252363/71‑1 (MQ=255)
  tGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                                    >  1:644274/1‑70 (MQ=255)
    ggAGTGCTTATTTTTGTATTTCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGAt                                                                 >  1:4515618/1‑71 (MQ=255)
    ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                                  <  1:5462382/70‑1 (MQ=255)
    ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                                  <  1:222583/70‑1 (MQ=255)
    ggAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                                  <  1:141501/70‑1 (MQ=255)
       gTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                                                              <  1:812098/71‑1 (MQ=255)
         gCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                                    <  1:1895950/63‑1 (MQ=255)
           tGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGc                                                              <  1:1624129/67‑1 (MQ=255)
              tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                       >  1:4984488/1‑71 (MQ=255)
               cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                                                                 >  1:1338825/1‑60 (MQ=255)
               cTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGAt                                                                 >  1:865341/1‑60 (MQ=255)
                  cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                                        <  1:2519598/66‑1 (MQ=255)
                  cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                                        <  1:5167353/66‑1 (MQ=255)
                  cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                                    <  1:467712/70‑1 (MQ=255)
                  cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                                    >  1:3327599/1‑70 (MQ=255)
                   gtatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                                  >  1:1521763/4‑71 (MQ=255)
                   gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                                   <  1:789457/70‑1 (MQ=255)
                   gTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                                   <  1:5359199/70‑1 (MQ=255)
                    tatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                                  <  1:27112/68‑1 (MQ=255)
                    tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCt                                                                    <  1:4594536/52‑1 (MQ=255)
                    tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                                    <  1:735993/68‑1 (MQ=255)
                    tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                                    <  1:4991647/68‑1 (MQ=255)
                    tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                                    <  1:4690396/68‑1 (MQ=255)
                    tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                                 <  1:851204/71‑1 (MQ=255)
                    tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                                 >  1:782962/1‑71 (MQ=255)
                    tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                                 >  1:2122634/1‑71 (MQ=255)
                      tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCa                                                   <  1:4724949/67‑1 (MQ=255)
                      tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                               >  1:2630246/1‑71 (MQ=255)
                      tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                               >  1:2794752/1‑71 (MQ=255)
                      tttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCa                                               >  1:4881032/1‑71 (MQ=255)
                        tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc                                                <  1:3938435/68‑1 (MQ=255)
                        tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                               <  1:2648889/69‑1 (MQ=255)
                        tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc                                             >  1:585691/1‑71 (MQ=255)
                        tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCaaaaaa                                                       >  1:1709133/1‑60 (MQ=255)
                        tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                                  >  1:1493973/1‑66 (MQ=255)
                            gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc                                          <  1:4729617/70‑1 (MQ=255)
                            gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc                                          <  1:2434928/70‑1 (MQ=255)
                            gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa                                         <  1:1255475/71‑1 (MQ=255)
                            gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa                                         <  1:3876165/71‑1 (MQ=255)
                             tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                                        >  1:4860180/1‑71 (MQ=255)
                             tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                                        >  1:5042565/1‑71 (MQ=255)
                             tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                                        >  1:2705846/1‑71 (MQ=255)
                               aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                                                         >  1:4173963/1‑52 (MQ=255)
                               aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg                                       <  1:5529270/70‑1 (MQ=255)
                                 tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                                                         <  1:2319478/50‑1 (MQ=255)
                                 tttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCaaaa                                                         <  1:2109396/50‑1 (MQ=255)
                                  ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                                  >  1:1130441/1‑40 (MQ=255)
                                  ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                                  >  1:1020948/1‑40 (MQ=255)
                                  ttttATGGAATCATCGCGATACATGACATCCCTTATCTGa                                                                  >  1:2328510/1‑40 (MQ=255)
                                   tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                            >  1:3092708/1‑61 (MQ=255)
                                   tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                            >  1:3497020/1‑61 (MQ=255)
                                    ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCg                                    >  1:114063/1‑68 (MQ=255)
                                    ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGAc                                  >  1:5043405/1‑70 (MQ=255)
                                    ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                                 >  1:1469594/1‑71 (MQ=255)
                                       tGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                              <  1:2605352/71‑1 (MQ=255)
                                         gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                            >  1:2599164/1‑71 (MQ=255)
                                          aaTCATCGCGATACATGACATCCCTTATCTGATTGCCaaaaa                                                        >  1:4549641/1‑42 (MQ=255)
                                            tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa                          >  1:3992792/1‑70 (MQ=255)
                                              atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                       >  1:5237444/1‑39 (MQ=255)
                                              atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                       >  1:3527576/1‑39 (MQ=255)
                                              atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                             <  1:2776419/65‑1 (MQ=255)
                                              atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa                        >  1:1243472/1‑70 (MQ=255)
                                               tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                                 >  1:1004806/1‑60 (MQ=255)
                                               tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg                      >  1:3178585/1‑71 (MQ=255)
                                                cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCGACCATCCCCATGCCGACGCTATTc                           >  1:5203480/1‑65 (MQ=255)
                                                cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                   >  1:3722708/1‑57 (MQ=255)
                                                cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                   >  1:2050557/1‑57 (MQ=255)
                                                cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                            >  1:1287173/1‑64 (MQ=255)
                                                cgcgATACATGACATCCCTTATCTGATTGCAAAAAAGCGCAACCATCCCCATGCCGa                                   >  1:1172349/1‑57 (MQ=255)
                                                 gcgATACATGACATCCCTTATCTGATTGCCAAAAAGAGCAACTATCCCCATGCCGACGCTATTCATAcggc                    >  1:2421094/1‑71 (MQ=255)
                                                   gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcgg                  <  1:4708106/71‑1 (MQ=255)
                                                     tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc                                <  1:2350146/55‑1 (MQ=255)
                                                     tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc                    <  1:2005412/67‑1 (MQ=255)
                                                     tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc                    <  1:1745259/67‑1 (MQ=255)
                                                       cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                                 <  1:811494/52‑1 (MQ=255)
                                                       cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                                 <  1:1092572/52‑1 (MQ=255)
                                                       cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                           <  1:1594965/42‑1 (MQ=255)
                                                       cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                           <  1:3715975/42‑1 (MQ=255)
                                                       cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                              >  1:477828/1‑55 (MQ=255)
                                                       cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                              >  1:1651559/1‑55 (MQ=255)
                                                       cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg                   >  1:3457701/1‑66 (MQ=255)
                                                              tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc       <  1:4346774/71‑1 (MQ=255)
                                                                ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                   >  1:2946378/1‑41 (MQ=255)
                                                                ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                   >  1:3127835/1‑41 (MQ=255)
                                                                ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt                         >  1:3402966/1‑51 (MQ=255)
                                                                ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt                         >  1:4697492/1‑51 (MQ=255)
                                                                ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc       <  1:3576495/69‑1 (MQ=255)
                                                                ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt     >  1:2166381/1‑71 (MQ=255)
                                                                 cTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg    >  1:4444284/1‑71 (MQ=255)
                                                                  ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                              >  1:649719/1‑44 (MQ=255)
                                                                   tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                   >  1:1926275/1‑38 (MQ=255)
                                                                   tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                   >  1:3921082/1‑38 (MQ=255)
                                                                   tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                                 <  1:2734561/40‑1 (MQ=255)
                                                                   tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt  >  1:3100864/1‑71 (MQ=255)
                                                                    aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                              >  1:3741527/1‑42 (MQ=255)
                                                                    aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa                          >  1:1366089/1‑46 (MQ=255)
                                                                    aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt  >  1:5463544/1‑70 (MQ=255)
                                                                     tCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcgg                     >  1:802442/1‑50 (MQ=255)
                                                                     |                                                                    
GTTGGGAGTGCTGATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTT  >  Exported/2569751‑2569888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: