Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,835 G→A 18.2% K35K (AAG→AAA yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,8350GA18.2% 366.6 / 63.2 165K35K (AAG→AAAyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (79/56);  new base A (19/11);  total (98/67)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

TCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCT  >  Exported/2569765‑2569905
                                                                      |                                                                      
tCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                                        >  1:3244098/1‑71 (MQ=255)
    cGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                                                     <  1:467712/70‑1 (MQ=255)
     gtatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                                                   >  1:1521763/4‑71 (MQ=255)
      tatttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaa                                                                   <  1:27112/68‑1 (MQ=255)
      tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAgcgc                                                                     <  1:735993/68‑1 (MQ=255)
      tCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                                                  >  1:782962/1‑71 (MQ=255)
       cTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAc                                                                  >  1:1727243/1‑70 (MQ=255)
       atttCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTaat                                                                  >  1:253195/2‑69 (MQ=255)
        tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                                                >  1:2794752/1‑71 (MQ=255)
        tttCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                                                >  1:2630246/1‑71 (MQ=255)
          tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc                                                                 <  1:4721837/68‑1 (MQ=255)
          tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAAcc                                                                 <  1:3938435/68‑1 (MQ=255)
          tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCa                                                                <  1:2648889/69‑1 (MQ=255)
          tCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATc                                                              >  1:585691/1‑71 (MQ=255)
          tCTGGTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAatcatc                                                              >  1:2103782/1‑71 (MQ=255)
              gTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcccc                                                           <  1:4729617/70‑1 (MQ=255)
              gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa                                                          <  1:1255475/71‑1 (MQ=255)
              gTGATCTTCTATGGGATCATTATTTTACATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCa                                                          <  1:3876165/71‑1 (MQ=255)
               tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                                                         >  1:5042565/1‑71 (MQ=255)
               tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                                                         >  1:4860180/1‑71 (MQ=255)
               tGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCAt                                                         >  1:2705846/1‑71 (MQ=255)
                 aTTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATg                                                        <  1:5529270/70‑1 (MQ=255)
                    ttttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                                        >  1:190146/1‑51 (MQ=255)
                     tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                                        >  1:224250/1‑50 (MQ=255)
                     tttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATcc                                                             >  1:3497020/1‑61 (MQ=255)
                      ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                                        >  1:3549900/1‑49 (MQ=255)
                      ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                                        >  1:2437681/1‑49 (MQ=255)
                      ttATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                                                  >  1:797799/1‑71 (MQ=255)
                        aTGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc                                                 >  1:3002362/1‑70 (MQ=255)
                         tGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                               <  1:2605352/71‑1 (MQ=255)
                           gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                                        >  1:4703683/1‑44 (MQ=255)
                           gAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAtt                                             >  1:4691944/1‑71 (MQ=255)
                              tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                                              <  1:2111433/67‑1 (MQ=255)
                              tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCa                                           >  1:3992792/1‑70 (MQ=255)
                              tcatcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt                                          <  1:1797360/71‑1 (MQ=255)
                                atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAg                                                                        >  1:5237444/1‑39 (MQ=255)
                                atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                                              <  1:2776419/65‑1 (MQ=255)
                                atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa                                         >  1:1243472/1‑70 (MQ=255)
                                atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa                                         >  1:852705/1‑70 (MQ=255)
                                atcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATa                                         <  1:1477907/70‑1 (MQ=255)
                                 tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                                                  >  1:1004806/1‑60 (MQ=255)
                                 tcGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcg                                       >  1:3178585/1‑71 (MQ=255)
                                  cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                                    >  1:2711969/1‑57 (MQ=255)
                                  cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                                    >  1:3722708/1‑57 (MQ=255)
                                  cgcgATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc                                            >  1:1299216/1‑65 (MQ=255)
                                  cgcgATACATGACATCCCTTATCTGATTGCAAAAAAGCGCAACCATCCCCATGCCGa                                                    >  1:1172349/1‑57 (MQ=255)
                                   gcgATACATGACATCCCTTATCTGATTGCCAAAAAGAGCAACTATCCCCATGCCGACGCTATTCATAcggc                                     >  1:2421094/1‑71 (MQ=255)
                                     gATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                                            <  1:576693/46‑1 (MQ=255)
                                       tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGc                                                 <  1:2350146/55‑1 (MQ=255)
                                       tACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggc                                     <  1:2005412/67‑1 (MQ=255)
                                         cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                                                  <  1:1127274/52‑1 (MQ=255)
                                         cATGATATTCCCTACCTGATTGCCAAAAAACGTAATCATCCTCATGCCGACg                                                  <  1:811494/52‑1 (MQ=255)
                                         cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATccc                                                            <  1:1594965/42‑1 (MQ=255)
                                         cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                               >  1:477828/1‑55 (MQ=255)
                                         cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                               >  1:1651559/1‑55 (MQ=255)
                                         cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATAcggcg                                    >  1:3457701/1‑66 (MQ=255)
                                         cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg                               >  1:4990284/1‑71 (MQ=255)
                                          aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGcc                                                      <  1:4485198/47‑1 (MQ=255)
                                           tGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTggg                             <  1:3918818/71‑1 (MQ=255)
                                                tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                               <  1:700276/48‑1 (MQ=255)
                                                tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc                        <  1:4346774/71‑1 (MQ=255)
                                                  ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGa                                                    >  1:3127835/1‑41 (MQ=255)
                                                  ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCAt                                          >  1:3402966/1‑51 (MQ=255)
                                                  ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                      <  1:2332947/71‑1 (MQ=255)
                                                  ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                      >  1:2166381/1‑71 (MQ=255)
                                                   cTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg                     >  1:4444284/1‑71 (MQ=255)
                                                    ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                               >  1:1282397/1‑44 (MQ=255)
                                                    ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                               >  1:649719/1‑44 (MQ=255)
                                                     tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACg                                                  <  1:2734561/40‑1 (MQ=255)
                                                     tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc                                            >  1:1708686/1‑46 (MQ=255)
                                                     tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTc                                            >  1:4624941/1‑46 (MQ=255)
                                                     tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                              <  1:3690337/60‑1 (MQ=255)
                                                     tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                   >  1:3100864/1‑71 (MQ=255)
                                                      accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                   >  1:3829765/3‑70 (MQ=255)
                                                      accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                   >  1:1425786/3‑70 (MQ=255)
                                                      accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                   >  1:4720228/3‑70 (MQ=255)
                                                      aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTa                                               >  1:3741527/1‑42 (MQ=255)
                                                      aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                   >  1:5463544/1‑70 (MQ=255)
                                                      aTCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                   <  1:1084463/70‑1 (MQ=255)
                                                       ccTGATTGCCAAAAAACGTAATCATCCTCATGCCGa                                                    >  1:831240/2‑36 (MQ=16)
                                                       ccTGATTGCCAAAAAACGTAATCATCCTCATGCCGa                                                    >  1:2995784/2‑36 (MQ=16)
                                                       ccTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                   <  1:3537745/68‑1 (MQ=255)
                                                        cTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg                     <  1:5044695/66‑1 (MQ=255)
                                                        cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTtttt                  <  1:4888679/69‑1 (MQ=255)
                                                         tGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTAt                                              >  1:2862651/1‑40 (MQ=255)
                                                           aTTGCCAAAAAGCGCAACCATCCTCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTAc                <  1:5367204/68‑1 (MQ=255)
                                                           aTTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct             >  1:2202953/1‑71 (MQ=255)
                                                           aTTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCa                                           >  1:5217061/1‑41 (MQ=19)
                                                                cAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATg        >  1:4926543/1‑71 (MQ=255)
                                                                 aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt       >  1:3214145/1‑71 (MQ=255)
                                                                  aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTa                 >  1:5133009/1‑60 (MQ=255)
                                                                  aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTa                 >  1:933933/1‑60 (MQ=255)
                                                                  aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt       >  1:435764/1‑70 (MQ=255)
                                                                  aaaaACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt       >  1:4942626/1‑70 (MQ=255)
                                                                    aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGTCTGGGTGAGCCTGTTTACTCTGCATGTTAt    >  1:308777/1‑71 (MQ=255)
                                                                    aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt    >  1:1535867/1‑71 (MQ=255)
                                                                    aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt    >  1:1164799/1‑71 (MQ=255)
                                                                     aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc   <  1:22948/71‑1 (MQ=255)
                                                                     aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc   <  1:5297502/71‑1 (MQ=255)
                                                                      gcgcAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCt  <  1:79918/71‑1 (MQ=255)
                                                                      |                                                                      
TCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCT  >  Exported/2569765‑2569905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: