Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,569,874 C→T 30.4% G48G (GGC→GGT yibI → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,569,8740CT30.4% 187.9 / 101.3 138G48G (GGC→GGTyibIpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (45/51);  new base T (22/20);  total (67/71)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

CATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCG  >  Exported/2569806‑2569943
                                                                    |                                                                     
cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCt                                                                      <  1:2381209/70‑1 (MQ=255)
cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg                                                                     >  1:2695250/1‑71 (MQ=255)
cATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTg                                                                     >  1:4990284/1‑71 (MQ=255)
 aTGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                                                                    <  1:812775/71‑1 (MQ=255)
  tGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTggg                                                                   <  1:3918818/71‑1 (MQ=255)
       tCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGc                                                              <  1:4346774/71‑1 (MQ=255)
         ccTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                                                            <  1:2332947/71‑1 (MQ=255)
           ttATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGt                                                          >  1:2197050/1‑71 (MQ=255)
            tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                                                                    <  1:3690337/60‑1 (MQ=255)
            tATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                                         >  1:3801171/1‑71 (MQ=255)
             accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                                         >  1:4720228/3‑70 (MQ=255)
             accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                                         >  1:3829765/3‑70 (MQ=255)
             accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                                         >  1:5464106/3‑70 (MQ=255)
             accTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                                         >  1:1425786/3‑70 (MQ=255)
             aTCTGATTGCCAAAAAGCGCAACCATCCCAATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                                         >  1:1190558/1‑70 (MQ=255)
              ccTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCtttt                                                         <  1:3537745/68‑1 (MQ=255)
               cTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTg                                                           <  1:5044695/66‑1 (MQ=255)
               cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCttt                                                          <  1:2727207/67‑3 (MQ=255)
               cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTtttt                                                        <  1:4888679/69‑1 (MQ=255)
               cTGATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAc                                                      <  1:1573822/71‑1 (MQ=255)
                 gATTGCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt                                                    >  1:5291234/1‑69 (MQ=255)
                  aTTGCCAAAAAGCGCAACCATCCTCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTAc                                                      <  1:5367204/68‑1 (MQ=255)
                   ttGCCGAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTActct                                                   >  1:3191869/1‑70 (MQ=255)
                   ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg                                                  <  1:4366441/71‑1 (MQ=255)
                   ttGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg                                                  >  1:1663345/1‑71 (MQ=255)
                    tGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTg                                                  >  1:992449/1‑70 (MQ=255)
                     gCCAAAAAACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTa                                                       >  1:3065113/1‑64 (MQ=255)
                       cAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATg                                              >  1:4926543/1‑71 (MQ=255)
                        aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                                             >  1:3214145/1‑71 (MQ=255)
                        aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                                             >  1:1269569/1‑71 (MQ=255)
                        aaaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                                             >  1:660056/1‑71 (MQ=255)
                         aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTa                                                       >  1:5133009/1‑60 (MQ=255)
                         aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                                             >  1:722329/1‑70 (MQ=255)
                         aaaaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGt                                             >  1:435764/1‑70 (MQ=255)
                         aaaaACGTAATCATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt                                             >  1:4942626/1‑70 (MQ=255)
                           aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGTCTGGGTGAGCCTGTTTACTCTGCATGTTAt                                          >  1:308777/1‑71 (MQ=255)
                           aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTgg                                                                    >  1:780185/1‑45 (MQ=255)
                           aaGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTAt                                          >  1:1164799/1‑71 (MQ=255)
                            aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc                                         <  1:22948/71‑1 (MQ=255)
                            aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc                                         <  1:5631368/71‑1 (MQ=255)
                            aGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATc                                         <  1:5297502/71‑1 (MQ=255)
                                  atcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                                           >  1:1354295/3‑63 (MQ=255)
                                   tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                                           <  1:768040/61‑1 (MQ=255)
                                   tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                                           <  1:5340909/61‑1 (MQ=255)
                                   tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTAt                                          >  1:3518308/2‑63 (MQ=255)
                                   tcATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGt                                  <  1:1009023/70‑1 (MQ=255)
                                   ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                                  <  1:4374643/71‑1 (MQ=255)
                                   ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                                  <  1:2571623/71‑1 (MQ=255)
                                   ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                                  <  1:5276981/71‑1 (MQ=255)
                                   ccATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGt                                  <  1:660972/71‑1 (MQ=255)
                                    cATCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGt                                             >  1:2114441/1‑59 (MQ=255)
                                     aTCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGtt                                            >  1:3210049/1‑59 (MQ=255)
                                     aTCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGtt                                 >  1:4722256/1‑70 (MQ=255)
                                      tCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt                                                    <  1:1721333/50‑3 (MQ=255)
                                      tCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt                                                    <  1:1132009/50‑3 (MQ=255)
                                      tCCTCATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTAcgt                                                    <  1:2048407/50‑3 (MQ=255)
                                      tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGtt                                                         <  1:2935852/45‑1 (MQ=255)
                                      tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc                               <  1:1196356/71‑1 (MQ=255)
                                      tCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc                               <  1:1489890/71‑1 (MQ=255)
                                      accccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCt                                                            <  1:3960212/41‑1 (MQ=255)
                                        ctcATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtg                             >  1:2797549/3‑71 (MQ=255)
                                        cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtg                             <  1:2518199/71‑1 (MQ=255)
                                        cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCtg                             <  1:2724093/71‑1 (MQ=255)
                                        cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt                              <  1:1131859/70‑1 (MQ=255)
                                        cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt                              <  1:3068120/70‑1 (MQ=255)
                                        cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt                              >  1:4563581/1‑70 (MQ=255)
                                        cccATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt                              <  1:3899699/70‑1 (MQ=255)
                                         tcATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTa                                           <  1:2215821/55‑1 (MQ=255)
                                         tcATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGttttt                              <  1:3591591/68‑3 (MQ=255)
                                          cATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCt                              <  1:1049063/68‑1 (MQ=255)
                                          cATGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtgtg                           >  1:4692436/1‑71 (MQ=255)
                                           aTGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtg                             <  1:5633290/68‑1 (MQ=255)
                                           aTGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTgg                          <  1:1260818/71‑1 (MQ=255)
                                            tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTAct                                                     <  1:3876431/43‑1 (MQ=255)
                                            tGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGa                         <  1:5629547/71‑1 (MQ=255)
                                            tGCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGa                         <  1:1399860/71‑1 (MQ=255)
                                             gCCGACGCTATTCATACGGCTGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGa                         <  1:469848/70‑1 (MQ=255)
                                             gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGttttt                              >  1:1053594/1‑63 (MQ=255)
                                             gCCGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTtg                             >  1:5564650/1‑66 (MQ=255)
                                              ccGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGAt                        >  1:1878292/1‑70 (MQ=255)
                                               cGACGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGAttt                      <  1:2189882/71‑3 (MQ=255)
                                                gACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTg                     <  1:912649/71‑1 (MQ=255)
                                                 aCGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGGCGTTTCTGTGGATCTg                     <  1:4249926/70‑1 (MQ=255)
                                                  cGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGt                                  <  1:3773534/56‑1 (MQ=255)
                                                  cGCCATTCATGTTGCTGGTTGGGTGAGTCTTTTTACGTTACATGTTATCTGGCCGTTTTTGTGGATTTggg                   <  1:3254356/71‑1 (MQ=255)
                                                      aTTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCa                                                <  1:2225296/38‑1 (MQ=255)
                                                       ttCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt                                            <  1:4987888/41‑1 (MQ=255)
                                                         cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACg              >  1:4651877/1‑69 (MQ=255)
                                                         cATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGc             >  1:876759/1‑70 (MQ=255)
                                                          aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt                                            <  1:2048063/38‑1 (MQ=255)
                                                          aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt                                            <  1:4698348/38‑1 (MQ=255)
                                                          aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt                                            <  1:35103/38‑1 (MQ=255)
                                                          aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGtt                                            <  1:4225046/38‑1 (MQ=255)
                                                          aTACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGctc           <  1:17661/71‑1 (MQ=255)
                                                            aCGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc                               <  1:49556/49‑1 (MQ=255)
                                                             cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGcc                                    >  1:2241659/1‑43 (MQ=255)
                                                             cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGcc                                    >  1:5406819/1‑43 (MQ=255)
                                                             cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGcc                                    >  1:3358285/1‑43 (MQ=255)
                                                             cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc                               >  1:3899144/1‑48 (MQ=255)
                                                             cggcggGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTc                               >  1:944097/1‑48 (MQ=255)
                                                                    |                                                                     
CATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGTTTCTGTGGATCTGGGCGACGCTCTATCAACCG  >  Exported/2569806‑2569943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: