Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,571,357 C→A 26.6% intergenic (+125/‑1654) yibH → / → ysaB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,571,3570CA26.6% 296.5 / 80.4 146intergenic (+125/‑1654)yibH/ysaBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (33/74);  new base A (21/18);  total (54/92)
Fisher's exact test for biased strand distribution p-value = 1.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

Only 100 of 151 total aligned reads displayed.

TATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGAT  >  Exported/2571289‑2571427
                                                                       |                                                                      
tatTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGctc                                                                      >  1:2352113/1‑71 (MQ=255)
 atTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGctct                                                                     >  1:5669681/1‑71 (MQ=255)
 atTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGctct                                                                     >  1:4021442/1‑71 (MQ=255)
        cATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGa                                                              >  1:2016698/1‑71 (MQ=255)
          ttAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAtt                                                            >  1:598891/1‑71 (MQ=255)
           tAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGTAtt                                                            <  1:225473/70‑1 (MQ=255)
            aGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGAttt                                                           <  1:2451652/70‑1 (MQ=255)
                 tgatgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGAtttctt                                                        <  1:4252406/69‑1 (MQ=255)
                  gatgatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATcc                                                    <  1:1022779/71‑1 (MQ=255)
                   atgatTTTTCCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATc                                                     >  1:3425292/1‑71 (MQ=255)
                   atgatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCAtt                                                                 <  1:1042987/57‑1 (MQ=255)
                     gatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATcc                                                    <  1:3671395/68‑1 (MQ=255)
                     gatTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                                                 >  1:3264253/1‑71 (MQ=255)
                     gatTTTACTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                                                 >  1:734041/1‑71 (MQ=255)
                      tttttcCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTc                                                 >  1:5093691/2‑71 (MQ=255)
                      atTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCtctc                                                                    <  1:2802838/51‑1 (MQ=255)
                      atTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTc                                                 <  1:2984344/70‑1 (MQ=255)
                        tttcCTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCtt                                               >  1:2110719/1‑71 (MQ=255)
                         tttCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTg                                              <  1:4212067/70‑1 (MQ=255)
                           tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                                              <  1:4726710/68‑1 (MQ=255)
                           tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                                              <  1:2989061/68‑1 (MQ=255)
                           tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                                              <  1:2353435/68‑1 (MQ=255)
                           tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                                              <  1:327799/68‑1 (MQ=255)
                           tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                                              <  1:2033084/68‑1 (MQ=255)
                           tccTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                                              <  1:529762/68‑1 (MQ=255)
                           tccTTTAATATCCAGCATGTTAAGAAGTTCTGGGATAGCTGTAATCTCATTAGATTTCTCATCCGTCTTg                                              <  1:1522883/68‑1 (MQ=255)
                           tccTTTAATATCCAGCATGTTAAGAAGTTCTGGGATAGCTGTAATCTCATTAGATTTCTCATCCGTCTTg                                              <  1:4309757/68‑1 (MQ=255)
                           tccTTTAATATCCAGCATGTTAAGAAGTTCTGGGATAGCTGTAATCTCATTAGATTTCTCATCCGTCTTg                                              <  1:2759612/68‑1 (MQ=255)
                           tccTTTAATATCCAGCATGTTAAGAAGTTCTGGGATAGCTGTAATCTCATTAGATTTCTCATCCGTCTTg                                              <  1:4254032/68‑1 (MQ=255)
                           tCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGtt                                            <  1:5695919/70‑1 (MQ=255)
                             cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc                                           >  1:4842888/1‑71 (MQ=255)
                             cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc                                           <  1:116806/71‑1 (MQ=255)
                              tttAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTc                                           <  1:475903/69‑1 (MQ=255)
                              tttAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                                              <  1:5480766/67‑1 (MQ=255)
                                tAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGGTCTGcc                                       >  1:5014420/1‑71 (MQ=255)
                                tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa                                                       >  1:2221760/1‑56 (MQ=255)
                                tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTa                                                       >  1:4814237/1‑56 (MQ=255)
                                tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCt                                          >  1:3759709/1‑69 (MQ=255)
                                tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTGc                                        >  1:2674080/1‑71 (MQ=255)
                                tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTGc                                        >  1:5528958/1‑71 (MQ=255)
                                tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTGc                                        >  1:3672754/1‑71 (MQ=255)
                                 aaTATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTgat                                            >  1:2205858/1‑64 (MQ=255)
                                  atatTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCg                                      <  1:1877765/70‑1 (MQ=255)
                                     ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATc                                                     >  1:5095556/1‑52 (MQ=255)
                                     ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATc                                                     >  1:4038816/1‑52 (MQ=255)
                                     ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCt                                          <  1:1611576/63‑1 (MQ=255)
                                     ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCt                                          <  1:1716614/63‑1 (MQ=255)
                                     ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAgt                                  <  1:5305193/71‑2 (MQ=255)
                                     ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg                                   <  1:1864496/70‑1 (MQ=255)
                                     ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg                                   <  1:3328060/70‑1 (MQ=255)
                                     ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg                                   <  1:1175257/70‑1 (MQ=255)
                                       cATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                                >  1:1549294/1‑71 (MQ=255)
                                        agcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc                                           >  1:5207379/4‑60 (MQ=255)
                                        agcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc                                           >  1:1731674/4‑60 (MQ=255)
                                        aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATcc                                                    >  1:482338/1‑50 (MQ=255)
                                        aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                                <  1:3930766/70‑1 (MQ=255)
                                        aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATg                               <  1:4985725/71‑1 (MQ=255)
                                        aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTATATCCGTCTTGTTCTGCCGCAGGAt                                <  1:5026670/70‑1 (MQ=255)
                                         ttATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGtt                                            <  1:4167997/57‑1 (MQ=255)
                                            tGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGAc                           <  1:4784700/71‑1 (MQ=255)
                                            tGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTg                                              <  1:4295897/53‑1 (MQ=255)
                                              ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATcc                                                    <  1:4938778/44‑1 (MQ=255)
                                              ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACta                         <  1:1616543/71‑1 (MQ=255)
                                              ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt                          <  1:1536982/70‑1 (MQ=255)
                                              ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt                          <  1:596909/70‑1 (MQ=255)
                                               taataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTa                                                       >  1:414453/1‑40 (MQ=255)
                                               taataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCt                                          >  1:3661204/1‑53 (MQ=255)
                                               taataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTg                                         >  1:4344942/1‑54 (MQ=255)
                                               taataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTg                                         >  1:4514969/1‑54 (MQ=255)
                                               taataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACta                         >  1:4223154/1‑70 (MQ=255)
                                                 ataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCg                                                   <  1:4732441/42‑1 (MQ=255)
                                                   aaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga                              >  1:246516/1‑61 (MQ=255)
                                                     gTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTc                                           <  1:1266936/46‑1 (MQ=255)
                                                        cTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc                <  1:3511341/70‑1 (MQ=255)
                                                          tGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc                <  1:3932380/69‑1 (MQ=255)
                                                          tGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc                <  1:774512/69‑1 (MQ=255)
                                                             gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                                >  1:2566562/1‑51 (MQ=255)
                                                             gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                                >  1:1469927/1‑51 (MQ=255)
                                                             gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt                                >  1:1823475/1‑51 (MQ=255)
                                                             gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc                <  1:5436525/67‑1 (MQ=255)
                                                             gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgaga            <  1:5619635/71‑1 (MQ=255)
                                                             gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag             <  1:3413843/70‑1 (MQ=255)
                                                             gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga              <  1:4358569/69‑1 (MQ=255)
                                                             gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga              <  1:4149641/69‑1 (MQ=255)
                                                             gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga              <  1:2161337/69‑1 (MQ=255)
                                                             gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGACGCAGGATGAGACTATACATCGTCgag             <  1:3829152/70‑1 (MQ=255)
                                                               tGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa                     <  1:1392556/60‑1 (MQ=255)
                                                                gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg         >  1:463623/1‑71 (MQ=255)
                                                                gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg         >  1:1537116/1‑71 (MQ=255)
                                                                 gctgtgATCTCATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTgagaa           >  1:1551697/3‑67 (MQ=255)
                                                                 aGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg         <  1:1820505/70‑1 (MQ=255)
                                                                  gtgtGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCa       <  1:227182/71‑1 (MQ=255)
                                                                     tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg                                   <  1:886221/40‑1 (MQ=255)
                                                                     tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg                                   <  1:3201858/40‑1 (MQ=255)
                                                                     tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg                                   <  1:5117893/40‑1 (MQ=255)
                                                                     tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg    <  1:1268388/71‑1 (MQ=255)
                                                                     tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg    <  1:3810209/71‑1 (MQ=255)
                                                                     tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat     <  1:4984534/70‑1 (MQ=255)
                                                                      gCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag             <  1:417316/61‑1 (MQ=255)
                                                                       ctctCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgat  <  1:151787/71‑1 (MQ=255)
                                                                       |                                                                      
TATTACTTCATTAGCTTCGATGATTTTTCTTTAATATTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGAT  >  Exported/2571289‑2571427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: