| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,571,384 | T→A | 23.3% | intergenic (+152/‑1627) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,571,384 | 0 | T | A | 23.3% | 332.0 / 102.3 | 168 | intergenic (+152/‑1627) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (48/80); new base A (26/13); total (75/93) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.68e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 177 total aligned reads displayed.
TTTCTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACG > Exported/2571314‑2571452 | tttCTTTAATATTCATTATGTTAATAAGTTC‑TCGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGt > 1:5527992/1‑71 (MQ=255) tCTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGtt < 1:5695919/70‑1 (MQ=255) cTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCt > 1:4191709/1‑71 (MQ=255) cTTTAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc < 1:116806/71‑1 (MQ=255) tttAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTc < 1:475903/69‑1 (MQ=255) tAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGcc > 1:109743/1‑71 (MQ=255) tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCt > 1:3759709/1‑69 (MQ=255) tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTGc > 1:4743825/1‑71 (MQ=255) tAATATCCAGCATGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATCTGc > 1:5528958/1‑71 (MQ=255) atatTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCg < 1:1877765/70‑1 (MQ=255) ttCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCt < 1:2512567/63‑1 (MQ=255) ttCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAgt < 1:5305193/71‑2 (MQ=255) agcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc > 1:5207379/4‑60 (MQ=255) agcaTGTTAAGAAGTTCAGGGATAGCTGTGATCTCATTGGATTTCTTATCCGTCTTGATc > 1:1731674/4‑60 (MQ=255) aTTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt < 1:3930766/70‑1 (MQ=255) aTTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATg < 1:4985725/71‑1 (MQ=255) aTTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTATATCCGTCTTGTTCTGCCGCAGGAt < 1:5026670/70‑1 (MQ=255) ttAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACta < 1:1616543/71‑1 (MQ=255) ttAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt < 1:596909/70‑1 (MQ=255) ttAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt < 1:1536982/70‑1 (MQ=255) taataaGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTg > 1:4344942/1‑54 (MQ=255) ataaGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGt < 1:4440026/48‑1 (MQ=255) c‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:5004618/70‑1 (MQ=255) c‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:3511341/70‑1 (MQ=255) tGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:3932380/69‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt > 1:2566562/1‑51 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag < 1:3413843/70‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga < 1:666272/69‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga < 1:4149641/69‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga < 1:2161337/69‑1 (MQ=255) tGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa < 1:1392556/60‑1 (MQ=255) tGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa < 1:3179875/60‑1 (MQ=255) gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg > 1:1537116/1‑71 (MQ=255) gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg > 1:3667859/1‑71 (MQ=255) gctgtgATCTCATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTgagaa > 1:1551697/3‑67 (MQ=255) aGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga < 1:1982580/49‑1 (MQ=255) aGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg < 1:1820505/70‑1 (MQ=255) gtgtGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCa < 1:227182/71‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg < 1:3201858/40‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg < 1:5117893/40‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg < 1:886221/40‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg < 1:1268388/71‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg < 1:3810209/71‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg < 1:4960781/71‑1 (MQ=255) gCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag < 1:4165716/61‑1 (MQ=255) gCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag < 1:417316/61‑1 (MQ=255) ctctCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgat < 1:151787/71‑1 (MQ=255) atctcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACg < 1:1855778/63‑1 (MQ=255) atctcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACg < 1:4993226/63‑1 (MQ=255) tctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata > 1:814179/1‑49 (MQ=255) ctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg < 1:1266480/36‑1 (MQ=255) ctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg < 1:1470732/36‑1 (MQ=255) ctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg < 1:398488/36‑1 (MQ=255) ctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg < 1:1381712/36‑1 (MQ=255) ctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga > 1:3864690/1‑41 (MQ=255) ctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga > 1:2578423/1‑41 (MQ=255) ctcATTAGATTTCTCATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatga > 1:2317238/1‑71 (MQ=255) tcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga > 1:4314960/1‑70 (MQ=255) tcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCacta < 1:4315466/66‑4 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata > 1:510010/1‑46 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg < 1:1937277/68‑1 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGata > 1:2811266/1‑71 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGata > 1:1951030/1‑71 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:3581123/68‑1 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTgagaa > 1:1460628/1‑56 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCActaatg < 1:4527043/67‑1 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:1715371/1‑71 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:2753796/1‑71 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga < 1:4703125/37‑1 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCa < 1:1107040/60‑1 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:5173566/62‑1 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgat < 1:5399116/63‑1 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:4112445/1‑62 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAg > 1:4664338/1‑71 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTTTGCATTGTTGAGAACGCACTAATGACATGa < 1:5040939/70‑1 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:947627/70‑1 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:1182047/70‑1 (MQ=255) gATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgt > 1:383292/1‑71 (MQ=255) gATTTCTTATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGaat > 1:3122013/1‑69 (MQ=255) ttttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATg < 1:2994731/66‑1 (MQ=255) tttcttATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCa > 1:4599096/1‑55 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtcg < 1:1363715/46‑1 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGc < 1:4813418/53‑1 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg > 1:2172702/1‑60 (MQ=255) tttATCGGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCaa > 1:731348/1‑53 (MQ=255) tttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtc > 1:2823603/1‑71 (MQ=255) tATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtcc < 1:2406726/70‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:3553576/55‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:1538276/55‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtcctcc > 1:2529112/1‑71 (MQ=255) cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTcctcct < 1:5342248/70‑1 (MQ=255) cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg > 1:3330444/1‑71 (MQ=255) cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg > 1:5491644/1‑71 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg < 1:5372862/69‑1 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc > 1:1955615/1‑71 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc > 1:1260997/1‑71 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCG‑AATGATGATATGAGTTGTTCCTCCTGCGACg > 1:1619006/1‑71 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCG‑AATGATGATATGAGTTGTTCCTCCTGCGACg > 1:1625714/1‑71 (MQ=255) ttCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga > 1:3234548/1‑46 (MQ=255) ttCTGCCCCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga > 1:4292406/1‑46 (MQ=255) | TTTCTTTAATATTCATTATGTTAATAAGTTC‑TGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACG > Exported/2571314‑2571452 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |