| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,571,423 | A→C | 13.2% | intergenic (+191/‑1588) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,571,423 | 0 | A | C | 13.2% | 224.9 / 33.4 | 106 | intergenic (+191/‑1588) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (53/39); new base C (8/6); total (61/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
Only 100 of 130 total aligned reads displayed.
TGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGCTTTTCCATC > Exported/2571355‑2571488 | tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg < 1:1268388/71‑1 (MQ=255)tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg < 1:3810209/71‑1 (MQ=255)tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg < 1:4960781/71‑1 (MQ=255)tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:4984534/70‑1 (MQ=255) ctctCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgat < 1:151787/71‑1 (MQ=255) ctcATTAGATTTCTCATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatga > 1:1943869/1‑71 (MQ=255) tcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga > 1:4314960/1‑70 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg < 1:3904393/68‑1 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg < 1:1937277/68‑1 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGata > 1:2811266/1‑71 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGata > 1:1951030/1‑71 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:552476/68‑1 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCActaatg < 1:4527043/67‑1 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatgaca < 1:2542554/70‑3 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatgaca < 1:3093088/70‑3 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatgaca < 1:4734283/70‑3 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:1715371/1‑71 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:3556466/1‑71 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:2753796/1‑71 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:5173566/62‑1 (MQ=255) tGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat > 1:5176006/1‑69 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgat < 1:5399116/63‑1 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:4112445/1‑62 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAg > 1:4664338/1‑71 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAg > 1:2577682/1‑71 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:947627/70‑1 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:1182047/70‑1 (MQ=255) gATTTCTTATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGaat > 1:3122013/1‑69 (MQ=255) ttttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATg < 1:231130/66‑1 (MQ=255) ttttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATg < 1:2994731/66‑1 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg > 1:3515198/1‑60 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg > 1:2172702/1‑60 (MQ=255) ttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgttgtt > 1:622813/1‑71 (MQ=255) tttATCGGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCaa > 1:731348/1‑53 (MQ=255) tttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtc > 1:2823603/1‑71 (MQ=255) tATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtcc < 1:2406726/70‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:5384567/55‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:3553576/55‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:1538276/55‑1 (MQ=255) cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTcctcct < 1:5342248/70‑1 (MQ=255) cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg > 1:5491644/1‑71 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg < 1:3229031/69‑1 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat > 1:4034113/1‑52 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCG‑AATGATGATATGAGTTGTTCCTCCTGCGACg > 1:1619006/1‑71 (MQ=255) gTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc < 1:1230706/69‑1 (MQ=255) ttCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga > 1:3234548/1‑46 (MQ=255) ttCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:4301107/46‑1 (MQ=255) tCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCaa > 1:2466197/1‑39 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:1971366/44‑1 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctc > 1:423132/1‑71 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGct > 1:3810355/1‑70 (MQ=255) tGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:3935302/38‑1 (MQ=255) tGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:64923/38‑1 (MQ=255) tGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACgg > 1:5083692/1‑67 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:1664118/1‑38 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:2298830/1‑38 (MQ=255) cAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa < 1:4786608/71‑1 (MQ=255) aGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc < 1:3721742/59‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgtt < 1:4446608/43‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgtt < 1:3708620/43‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTa < 1:5339379/64‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:5505732/71‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:3290580/71‑1 (MQ=255) aTGAGACTATACATCGTCGAGATCGCAATGATGATATGAgtt < 1:4218532/42‑1 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTaa > 1:5210211/1‑63 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa > 1:2546351/1‑69 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa > 1:4208551/1‑66 (MQ=255) tGAGACGATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTaa > 1:1459/1‑63 (MQ=255) aCTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaaaa > 1:4354855/1‑67 (MQ=255) aCTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAagag > 1:242859/1‑70 (MQ=255) tataCATCGTCGAGATCGCAATGATGTTATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAg > 1:3801726/1‑71 (MQ=255) tataCATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaaaa > 1:171021/1‑65 (MQ=255) taCATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCTACGGCTCTAATCAAAAAAGAGCAgg < 1:3670887/70‑1 (MQ=255) taCATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAAt < 1:5045480/56‑1 (MQ=255) taCATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAgg < 1:2745143/70‑1 (MQ=255) taCATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAg > 1:4592541/1‑69 (MQ=255) taCATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAg > 1:2680435/1‑69 (MQ=255) cATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctc > 1:63521/1‑50 (MQ=255) cATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa > 1:4450503/1‑56 (MQ=255) cATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa > 1:3092649/1‑56 (MQ=255) cATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGt > 1:4219500/1‑71 (MQ=255) aTCGTCGAGATCGCAATGATGATATGAGTTGTtcct > 1:4080725/1‑36 (MQ=255) aTCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAg < 1:1990394/66‑1 (MQ=255) tcgtcgAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGc > 1:1351916/1‑71 (MQ=255) tcgtcgAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGc > 1:4018276/1‑71 (MQ=255) tcgtcgAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGc > 1:3781592/1‑71 (MQ=255) tcgtcgAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGc > 1:224432/1‑71 (MQ=255) tcgtcgAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGc > 1:4684665/1‑71 (MQ=255) gagaTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAg > 1:5419034/1‑63 (MQ=255) gaTCGCAATGATGATATGAGTTGTTCCTCCTGCGAAGGCTCTaa > 1:1148926/1‑44 (MQ=255) aTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:2593939/49‑1 (MQ=255) aTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAag < 1:124382/52‑1 (MQ=255) aTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAag < 1:4736522/52‑1 (MQ=255) aTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAgg < 1:84878/58‑1 (MQ=255) aTCGCAATCATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:4850145/49‑1 (MQ=255) cGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGCTTTTCCATc > 1:1901843/1‑70 (MQ=255) aaTGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCTGGAGTGCtt > 1:1967423/1‑60 (MQ=255) aaTGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGCtt > 1:4438096/1‑60 (MQ=255) atgatgATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:3310542/43‑1 (MQ=255) atgatgATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa < 1:5639258/40‑1 (MQ=255) | TGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGCTTTTCCATC > Exported/2571355‑2571488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |