Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,572,772 T→C 19.7% intergenic (+1540/‑239) yibH → / → ysaB hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,572,7720TC19.7% 245.1 / 53.5 122intergenic (+1540/‑239)yibH/ysaBhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (46/52);  new base C (14/10);  total (60/62)
Fisher's exact test for biased strand distribution p-value = 3.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.58e-01

Only 100 of 133 total aligned reads displayed.

CACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGCTTCTG  >  Exported/2572703‑2572839
                                                                     |                                                                   
cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat                                                                     <  1:2640986/70‑1 (MQ=255)
  cctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt                                                                  <  1:2116581/71‑1 (MQ=255)
   ctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt                                                                  >  1:2631853/1‑70 (MQ=255)
    tacctTCCAGCCCAATCGGAGCCTGAGAGTTATACCGCCCCAGCTGCGGTTAAAGTTACCGAAAATAGAta                                                                >  1:2432791/1‑71 (MQ=255)
     acctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta                                                                >  1:2439551/1‑70 (MQ=255)
      cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTTCGGTTAATGTTACCGATAATAGTta                                                                <  1:3063471/69‑1 (MQ=255)
      cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta                                                                <  1:4763221/69‑1 (MQ=255)
      cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta                                                                <  1:5310379/69‑1 (MQ=255)
       ctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg                                                             <  1:5513053/71‑1 (MQ=255)
       ctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg                                                             >  1:1943673/1‑71 (MQ=255)
         tCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg                                                             >  1:1695969/1‑69 (MQ=255)
          ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTTCTGCGGTTAATGTTACCGATAATAGTTATAGt                                                            <  1:5113808/69‑1 (MQ=255)
          ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa                                                           <  1:5045414/70‑1 (MQ=255)
           cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa                                                           >  1:2144679/1‑69 (MQ=255)
           cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa                                                           >  1:1811317/1‑69 (MQ=255)
              ccccATCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGcc                                                      >  1:2273757/1‑71 (MQ=255)
              ccccATCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGcc                                                      <  1:1764035/71‑1 (MQ=255)
              cccAATCGGGTCCGGGGTGATATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGcc                                                      >  1:64164/1‑71 (MQ=255)
               cccaTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGccc                                                     >  1:1898110/4‑71 (MQ=255)
                cAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt                                                    <  1:28268/71‑1 (MQ=255)
                 caTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTg                                                   <  1:3753246/70‑1 (MQ=255)
                  aTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGa                                                  >  1:3363095/1‑71 (MQ=255)
                  aTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCACTGa                                                  >  1:3547067/1‑71 (MQ=255)
                   tCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAt                                                 >  1:4835131/1‑71 (MQ=255)
                   tCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAt                                                 >  1:2551937/1‑71 (MQ=255)
                     ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataata                                                                    >  1:4172927/1‑50 (MQ=255)
                     ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtc                                               <  1:3677966/71‑1 (MQ=255)
                        tccTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctc                                            <  1:2827269/70‑1 (MQ=255)
                         ccTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGt                                                            <  1:5209863/54‑1 (MQ=255)
                         ccTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatca                                           >  1:1458927/1‑71 (MQ=255)
                          cTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat                                          <  1:1215386/71‑1 (MQ=255)
                           tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGt                                                            <  1:1550200/52‑1 (MQ=255)
                           tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTAc                                                          >  1:3606836/1‑54 (MQ=255)
                           tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat                                          >  1:4255053/1‑70 (MQ=255)
                           tGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctcat                                          >  1:4848809/1‑70 (MQ=255)
                            gAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAg                                                                   <  1:5317765/44‑1 (MQ=255)
                             ggtgaTATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACTcctcatcat                                       <  1:3500579/70‑1 (MQ=255)
                             aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAg                                                                   <  1:375271/43‑1 (MQ=255)
                             aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca                                        <  1:4024081/70‑1 (MQ=255)
                             aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTcatcatcat                                       <  1:9140/71‑1 (MQ=255)
                              gtgtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt                                                >  1:1850727/1‑61 (MQ=255)
                              gtgtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt                                                >  1:4311450/1‑61 (MQ=255)
                              gtgaTATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACtcctcat                                          <  1:4888930/67‑1 (MQ=255)
                                gtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat                                          <  1:3750460/65‑1 (MQ=255)
                                gtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat                                          <  1:3995348/65‑1 (MQ=255)
                                aatataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCAtgct                                    >  1:3625482/3‑71 (MQ=255)
                                 ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt                                                    <  1:459614/54‑1 (MQ=255)
                                 ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt                                                    <  1:5120786/54‑1 (MQ=255)
                                 ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTcatcatcat                                       <  1:1729571/67‑1 (MQ=255)
                                 ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtact                                    <  1:704595/70‑1 (MQ=255)
                                 atataCCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGcccc                                                    >  1:1408611/2‑53 (MQ=255)
                                 atataCCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACTCCTCATCAtgctg                                   <  1:1260426/70‑2 (MQ=255)
                                 atataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctc                                            >  1:2377207/2‑62 (MQ=255)
                                 atataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCAtgctg                                   >  1:5647503/2‑70 (MQ=255)
                                   ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTtata                                                              >  1:1599657/1‑42 (MQ=255)
                                   ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTtat                                                               >  1:4843679/1‑41 (MQ=255)
                                   ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATagtact                                 >  1:311533/1‑71 (MQ=255)
                                    taCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGcc                                                      >  1:3381715/1‑49 (MQ=255)
                                    taCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGcc                                                      >  1:3598182/1‑49 (MQ=255)
                                    taCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCATGctgctg                                >  1:1084867/1‑71 (MQ=255)
                                     aCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat                                          <  1:3216827/60‑1 (MQ=255)
                                         ccccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCg                           >  1:1675088/1‑71 (MQ=255)
                                           ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGa                                                  >  1:707945/1‑46 (MQ=255)
                                           ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGa                                                  >  1:4672802/1‑46 (MQ=255)
                                           ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtact                                    <  1:4925133/60‑1 (MQ=255)
                                           ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATactactact                              <  1:2410494/66‑1 (MQ=255)
                                           ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCgg                          >  1:1158939/1‑70 (MQ=255)
                                            ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca                                        <  1:2079703/55‑1 (MQ=255)
                                            ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca                                        <  1:1131489/55‑1 (MQ=255)
                                            ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca                                        <  1:878015/55‑1 (MQ=255)
                                            ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca                                        <  1:3793395/55‑1 (MQ=255)
                                            ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCa                        <  1:3059692/71‑1 (MQ=255)
                                             tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca                                        <  1:4509804/54‑1 (MQ=255)
                                             tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca                                        <  1:4503456/54‑1 (MQ=255)
                                             tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca                                        <  1:3481780/54‑1 (MQ=255)
                                             tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca                                        <  1:105533/54‑1 (MQ=255)
                                             tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAg                       >  1:3132992/1‑71 (MQ=255)
                                              gctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat                                          <  1:1960161/51‑1 (MQ=255)
                                              gctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat                                          <  1:946158/51‑1 (MQ=255)
                                              gctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGc                         >  1:3048195/1‑68 (MQ=255)
                                               ctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGt                      <  1:4689544/70‑1 (MQ=255)
                                                 gcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGc                         >  1:5064551/1‑65 (MQ=255)
                                                     ttAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtgat                 <  1:5478436/69‑1 (MQ=255)
                                                     ttAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtgat                 <  1:2148182/69‑1 (MQ=255)
                                                      tAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGaa              >  1:587680/1‑71 (MQ=255)
                                                      tAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGaa              >  1:610744/1‑71 (MQ=255)
                                                       aaTGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATactact                                 >  1:2881159/1‑51 (MQ=255)
                                                       aaTGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGaa              >  1:1630218/1‑70 (MQ=255)
                                                            tACCGATAATAGTTATAGTACAGCCCTTATTCATCATCAta                                      >  1:5017773/1‑41 (MQ=255)
                                                            tACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAta                                      >  1:143625/1‑41 (MQ=255)
                                                            tACCGATAATAGTTATAGTACAGCCCTGATTAATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTg        >  1:3958912/1‑71 (MQ=255)
                                                              ccGATAATAGTTATAGTACAGCCCTGATTCATCATCAtact                                    <  1:4970106/41‑1 (MQ=255)
                                                              ccGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTa           >  1:1186802/1‑66 (MQ=255)
                                                              ccGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGCt      >  1:678455/1‑71 (MQ=255)
                                                                gATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGCttt    <  1:2099569/71‑2 (MQ=255)
                                                                  taataGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGCtttta  >  1:5567465/1‑68 (MQ=255)
                                                                  taataGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGCtttta  <  1:3806569/71‑4 (MQ=255)
                                                                    ataGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCg                           >  1:2202934/1‑44 (MQ=255)
                                                                    ataGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCg                           >  1:2367906/1‑44 (MQ=255)
                                                                    ataGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAg                       <  1:4014853/48‑1 (MQ=255)
                                                                     |                                                                   
CACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAACTATTGCTTCTG  >  Exported/2572703‑2572839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: