| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,584,324 | G→A | 23.5% | L985L (CTG→TTG) ‡ | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,584,324 | 0 | G | A | 23.5% | 243.4 / 60.6 | 115 | L985L (CTG→TTG) ‡ | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (44/44); new base A (11/16); total (55/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01 | |||||||||||
Only 100 of 118 total aligned reads displayed.
CCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATG > Exported/2584257‑2584391 | ccATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACg < 1:765931/71‑1 (MQ=255) cATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGc > 1:5622025/1‑71 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:5471326/1‑68 (MQ=255) ccGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGaa > 1:1165698/1‑70 (MQ=255) cgatacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAAt > 1:1730401/4‑70 (MQ=9) cgatacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATa < 1:2237258/68‑1 (MQ=9) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAAt > 1:1950990/1‑70 (MQ=255) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:5205726/71‑1 (MQ=255) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:2445552/71‑1 (MQ=255) gTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaaa < 1:1516042/71‑1 (MQ=255) gTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa > 1:2285179/1‑69 (MQ=255) atacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATaaa < 1:4123503/70‑1 (MQ=9) atacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATaaa < 1:852092/70‑1 (MQ=9) tacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAg < 1:4715207/71‑1 (MQ=9) accGCGTTTTGAGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCAc > 1:384676/1‑53 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:1625428/1‑69 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:2544561/1‑69 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:4008447/71‑1 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:1895414/71‑1 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGCCAGCACGCCGAGAATAAAGGCCagag < 1:768882/71‑1 (MQ=255) agcgTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGcga > 1:4694479/2‑66 (MQ=9) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:3908394/1‑61 (MQ=255) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:1059270/1‑61 (MQ=255) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:5171755/1‑61 (MQ=255) cgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag > 1:5291444/1‑71 (MQ=255) gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:4306235/67‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc < 1:4521918/50‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc < 1:4760626/50‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc < 1:4764709/50‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:562964/66‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa < 1:3214057/64‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa < 1:4791385/64‑1 (MQ=255) tGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtcat > 1:391086/1‑71 (MQ=255) gcgcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtcat < 1:3554749/70‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcatc < 1:4469410/70‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcat < 1:4900481/69‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcat < 1:129252/69‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcat < 1:1735021/69‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcat < 1:4289323/69‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGgcat < 1:4282731/69‑1 (MQ=255) cgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:3756472/71‑1 (MQ=255) gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa > 1:503638/1‑71 (MQ=255) gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa > 1:5537871/1‑71 (MQ=255) ccAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt < 1:162097/71‑1 (MQ=255) cAGAACCGGCACCATGACTGATAACCAGAGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAtt > 1:365942/1‑71 (MQ=255) tgccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGAt < 1:4171865/65‑1 (MQ=12) gccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCgaga > 1:4517874/2‑52 (MQ=255) gccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCgaga > 1:2230835/2‑52 (MQ=255) gccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCgaga > 1:1120242/2‑52 (MQ=255) ccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg < 1:1165271/71‑1 (MQ=9) ccGGCAACGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg > 1:735007/1‑71 (MQ=9) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:4758177/71‑1 (MQ=255) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:5418138/71‑1 (MQ=255) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:4030879/71‑1 (MQ=255) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:245938/71‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAAt < 1:2535918/38‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga < 1:4724158/50‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga < 1:3166582/50‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga < 1:5239277/50‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga < 1:1835747/50‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa > 1:3605538/1‑70 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATaaa > 1:4262358/1‑38 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATaaa > 1:345050/1‑38 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagtgag < 1:2822000/48‑1 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:3692448/57‑1 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:4370925/57‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:4535269/56‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa < 1:4750877/57‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa < 1:5678795/57‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:1370591/71‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:2464338/71‑1 (MQ=255) gttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGgacgcagacgca < 1:544853/68‑1 (MQ=2) gttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGgacgcagacgca > 1:5043168/4‑71 (MQ=2) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga > 1:1880683/1‑45 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:1698327/1‑41 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGt > 1:3468221/1‑48 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg > 1:66371/1‑55 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTg > 1:4813082/1‑59 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGACAGGTCATCAGGATTGGGCGTaa > 1:1121840/1‑66 (MQ=255) ttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACg > 1:1321823/3‑68 (MQ=9) taCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGa > 1:2929012/2‑54 (MQ=17) aCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGt > 1:1137416/1‑45 (MQ=16) cTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcg < 1:602202/71‑1 (MQ=9) tGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACgc < 1:1845112/59‑2 (MQ=9) tGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACgc < 1:3747188/59‑2 (MQ=9) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa > 1:2473156/1‑49 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:5475533/1‑60 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCAt > 1:3998547/1‑65 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcgcg > 1:4958936/1‑70 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCgcgc > 1:3171747/1‑71 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCgcgc > 1:1885653/1‑71 (MQ=255) aCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc > 1:377027/1‑71 (MQ=255) ccAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCt < 1:5679229/71‑1 (MQ=255) ccAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCt < 1:3438055/71‑1 (MQ=255) aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc < 1:1230076/71‑1 (MQ=255) ggCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgat < 1:2225644/71‑1 (MQ=255) ggCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCg > 1:2095808/1‑69 (MQ=255) gcagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatg < 1:126222/71‑1 (MQ=255) gcagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatg < 1:4232838/71‑1 (MQ=255) gcagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatg < 1:774471/71‑1 (MQ=255) | CCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATG > Exported/2584257‑2584391 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |