Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,584,343 C→G 20.0% L978L (CTG→CTC)  mdtF ← multidrug transporter, RpoS‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,584,3430CG20.0% 229.7 / 46.3 100L978L (CTG→CTC) mdtFmultidrug transporter, RpoS‑dependent
Reads supporting (aligned to +/- strand):  ref base C (35/45);  new base G (7/13);  total (42/58)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

Only 100 of 105 total aligned reads displayed.

CCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCC  >  Exported/2584276‑2584413
                                                                   |                                                                      
ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa                                                                       >  1:2544561/1‑69 (MQ=255)
ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa                                                                       >  1:1625428/1‑69 (MQ=255)
 cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag                                                                    <  1:1895414/71‑1 (MQ=255)
 cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag                                                                    <  1:4008447/71‑1 (MQ=255)
 cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGCCAGCACGCCGAGAATAAAGGCCagag                                                                    <  1:768882/71‑1 (MQ=255)
   cgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag                                                                  >  1:5291444/1‑71 (MQ=255)
    gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga                                                                     <  1:4306235/67‑1 (MQ=255)
     ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga                                                                     <  1:562964/66‑1 (MQ=255)
     ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga                                                                     <  1:5564802/66‑1 (MQ=255)
     ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa                                                                       <  1:3214057/64‑1 (MQ=255)
     ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa                                                                       <  1:4791385/64‑1 (MQ=255)
        tGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtcat                                                             >  1:391086/1‑71 (MQ=255)
          cgcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtcatc                                                            <  1:1793153/70‑1 (MQ=255)
          cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcatc                                                            <  1:4469410/70‑1 (MQ=255)
          cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcat                                                             <  1:129252/69‑1 (MQ=255)
          cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcat                                                             <  1:1735021/69‑1 (MQ=255)
          cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcat                                                             <  1:4900481/69‑1 (MQ=255)
          cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGgcat                                                             <  1:4282731/69‑1 (MQ=255)
            cgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg                                                         <  1:3756472/71‑1 (MQ=255)
             gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa                                                        >  1:5537871/1‑71 (MQ=255)
             gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa                                                        >  1:503638/1‑71 (MQ=255)
              ccAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt                                                       <  1:162097/71‑1 (MQ=255)
               cAGAACCGGCACCATGACTGATAACCAGAGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAtt                                                      >  1:365942/1‑71 (MQ=255)
                  tgccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGAt                                                       <  1:4171865/65‑1 (MQ=12)
                   gccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCgaga                                                                     >  1:4517874/2‑52 (MQ=255)
                   gccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCgaga                                                                     >  1:1120242/2‑52 (MQ=255)
                   gccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCgaga                                                                     >  1:2230835/2‑52 (MQ=255)
                    ccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg                                                 <  1:1165271/71‑1 (MQ=9)
                    ccGGCAACGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg                                                 >  1:735007/1‑71 (MQ=9)
                     cGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg                                                 >  1:20626/1‑70 (MQ=9)
                      ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                               <  1:4030879/71‑1 (MQ=255)
                      ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                               <  1:245938/71‑1 (MQ=255)
                      ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                               <  1:4758177/71‑1 (MQ=255)
                      ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                               <  1:5418138/71‑1 (MQ=255)
                       gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga                                                                   <  1:1835747/50‑1 (MQ=255)
                       gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga                                                                   <  1:3166582/50‑1 (MQ=255)
                       gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga                                                                   <  1:4724158/50‑1 (MQ=255)
                       gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga                                                                   <  1:4760639/50‑1 (MQ=255)
                       gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                               >  1:4600369/1‑70 (MQ=255)
                       gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                               >  1:3605538/1‑70 (MQ=255)
                          ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagtgag                                                                  <  1:2822000/48‑1 (MQ=255)
                          ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg                                                         <  1:3692448/57‑1 (MQ=255)
                          ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg                                                         <  1:4370925/57‑1 (MQ=255)
                          ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg                                           <  1:3864851/71‑1 (MQ=255)
                           cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg                                                         <  1:4535269/56‑1 (MQ=255)
                           cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa                                                        <  1:4750877/57‑1 (MQ=255)
                           cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa                                                        <  1:5678795/57‑1 (MQ=255)
                           cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                                          <  1:1370591/71‑1 (MQ=255)
                           cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                                          <  1:2464338/71‑1 (MQ=255)
                            gttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGgacgcagacgca                                         >  1:5043168/4‑71 (MQ=2)
                            gttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGgacgcagacgca                                         <  1:544853/68‑1 (MQ=2)
                            aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga                                                                   >  1:1880683/1‑45 (MQ=255)
                            aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa                                                                       >  1:1698327/1‑41 (MQ=255)
                            aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtc                                                               <  1:4150350/49‑1 (MQ=255)
                            aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGt                                                                >  1:946660/1‑48 (MQ=255)
                            aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGt                                                                >  1:3468221/1‑48 (MQ=255)
                            aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg                                                         >  1:66371/1‑55 (MQ=255)
                            aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTg                                                     >  1:4813082/1‑59 (MQ=255)
                            aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGACAGGTCATCAGGATTGGGCGTaa                                              >  1:1121840/1‑66 (MQ=255)
                             ttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACg                                           >  1:1321823/3‑68 (MQ=9)
                              taCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGa                                                        >  1:2929012/2‑54 (MQ=17)
                               aCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGt                                                                >  1:1137416/1‑45 (MQ=16)
                                cTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcg                                     <  1:602202/71‑1 (MQ=9)
                                 tGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACgc                                                <  1:3747188/59‑2 (MQ=9)
                                 tGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACgc                                                <  1:1845112/59‑2 (MQ=9)
                                   aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa                                                        >  1:2473156/1‑49 (MQ=255)
                                   aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa                                             >  1:5475533/1‑60 (MQ=255)
                                   aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaa                                              >  1:4321275/1‑59 (MQ=255)
                                   aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCAt                                        >  1:3998547/1‑65 (MQ=255)
                                   aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCAt                                        >  1:1528676/1‑65 (MQ=255)
                                   aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcgcg                                   >  1:4958936/1‑70 (MQ=255)
                                   aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCgcgc                                  >  1:3171747/1‑71 (MQ=255)
                                      aCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc                               >  1:377027/1‑71 (MQ=255)
                                       ccAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCt                              <  1:5679229/71‑1 (MQ=255)
                                       ccAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCt                              <  1:3438055/71‑1 (MQ=255)
                                         aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc                            <  1:1230076/71‑1 (MQ=255)
                                          gCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc                            >  1:1208272/1‑70 (MQ=255)
                                            ggCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgat                         <  1:2225644/71‑1 (MQ=255)
                                            ggCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCg                           >  1:2095808/1‑69 (MQ=255)
                                             gcagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatg                        <  1:774471/71‑1 (MQ=255)
                                             gcagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatg                        <  1:126222/71‑1 (MQ=255)
                                             gcagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatg                        <  1:4232838/71‑1 (MQ=255)
                                                  aCGCCGAGAATAAAGGCGAGAGAGGTCATCAGGAt                                                       >  1:4288444/1‑35 (MQ=255)
                                                    gCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATACGCGCCGCTTCgatga                       >  1:1508865/1‑65 (MQ=255)
                                                      cGAGAATAAAGGCGAGAGAGGTCATCAGGATAGgacgcagacgca                                         <  1:3597625/45‑1 (MQ=14)
                                                      cGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCctct               <  1:3884686/71‑1 (MQ=255)
                                                       gagaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTa              >  1:4662074/1‑71 (MQ=255)
                                                       gagaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTa              <  1:4799728/71‑1 (MQ=255)
                                                        agaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCga                          >  1:481579/1‑58 (MQ=255)
                                                         gaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATc            >  1:5569807/1‑71 (MQ=255)
                                                          aaTAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg                                           <  1:3102816/39‑1 (MQ=255)
                                                            tAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCt                              <  1:791186/50‑1 (MQ=255)
                                                            tAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGcc                  >  1:4039750/1‑62 (MQ=255)
                                                            tAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGcc                  >  1:2227228/1‑62 (MQ=255)
                                                            tAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGc         >  1:3886279/1‑71 (MQ=255)
                                                             aaaGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCg        >  1:83996/1‑71 (MQ=255)
                                                                 gCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGtttt    <  1:2129364/71‑1 (MQ=255)
                                                                 gCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGtttt    <  1:866093/71‑1 (MQ=255)
                                                                  ccAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCg        >  1:873139/1‑66 (MQ=255)
                                                                   cAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc  <  1:2919290/71‑1 (MQ=255)
                                                                   |                                                                      
CCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCC  >  Exported/2584276‑2584413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑