Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,584,361 T→A 13.9% P972P (CCA→CCT mdtF ← multidrug transporter, RpoS‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,584,3610TA13.9% 189.7 / 20.7 72P972P (CCA→CCTmdtFmultidrug transporter, RpoS‑dependent
Reads supporting (aligned to +/- strand):  ref base T (31/31);  new base A (4/6);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

CAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGCATC  >  Exported/2584291‑2584427
                                                                      |                                                                  
cAGAACCGGCACCATGACTGATAACCAGAGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAtt                                                                    >  1:365942/1‑71 (MQ=255)
     ccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg                                                               <  1:1165271/71‑1 (MQ=9)
     ccGGCAACGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg                                                               >  1:735007/1‑71 (MQ=9)
      cGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg                                                               >  1:20626/1‑70 (MQ=9)
       ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                                             <  1:5418138/71‑1 (MQ=255)
       ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                                             <  1:4758177/71‑1 (MQ=255)
       ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                                             <  1:4030879/71‑1 (MQ=255)
       ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                                             <  1:245938/71‑1 (MQ=255)
        gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                                             >  1:4600369/1‑70 (MQ=255)
        gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa                                                             >  1:3605538/1‑70 (MQ=255)
           ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg                                                         <  1:3864851/71‑1 (MQ=255)
            cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                                                        <  1:1370591/71‑1 (MQ=255)
            cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc                                                        <  1:2464338/71‑1 (MQ=255)
             gttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGgacgcagacgca                                                       <  1:544853/68‑1 (MQ=2)
             gttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGgacgcagacgca                                                       >  1:5043168/4‑71 (MQ=2)
             aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTg                                                                   >  1:4813082/1‑59 (MQ=255)
             aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGACAGGTCATCAGGATTGGGCGTaa                                                            >  1:1121840/1‑66 (MQ=255)
              ttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACg                                                         >  1:1321823/3‑68 (MQ=9)
                 cTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcg                                                   <  1:602202/71‑1 (MQ=9)
                  tGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACgc                                                              <  1:1845112/59‑2 (MQ=9)
                  tGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACgc                                                              <  1:3747188/59‑2 (MQ=9)
                    aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa                                                           >  1:5475533/1‑60 (MQ=255)
                    aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaa                                                            >  1:4321275/1‑59 (MQ=255)
                    aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCAt                                                      >  1:3998547/1‑65 (MQ=255)
                    aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCAt                                                      >  1:1528676/1‑65 (MQ=255)
                    aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcgcg                                                 >  1:4958936/1‑70 (MQ=255)
                    aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCgcgc                                                >  1:3171747/1‑71 (MQ=255)
                    aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCgcgc                                                >  1:1885653/1‑71 (MQ=255)
                       aCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc                                             >  1:377027/1‑71 (MQ=255)
                        ccAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCt                                            <  1:3438055/71‑1 (MQ=255)
                        ccAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCt                                            <  1:5679229/71‑1 (MQ=255)
                          aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc                                          <  1:1230076/71‑1 (MQ=255)
                           gCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc                                          >  1:1208272/1‑70 (MQ=255)
                             ggCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgat                                       <  1:2225644/71‑1 (MQ=255)
                             ggCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCg                                         >  1:2095808/1‑69 (MQ=255)
                              gcagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatg                                      <  1:126222/71‑1 (MQ=255)
                              gcagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatg                                      <  1:774471/71‑1 (MQ=255)
                              gcagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatg                                      <  1:4232838/71‑1 (MQ=255)
                                     gCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATACGCGCCGCTTCgatga                                     >  1:1508865/1‑65 (MQ=255)
                                       cGAGAATAAAGGCGAGAGAGGTCATCAGGATAGgacgcagacgca                                                       <  1:3597625/45‑1 (MQ=14)
                                       cGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCctct                             <  1:3884686/71‑1 (MQ=255)
                                        gagaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTa                            <  1:4799728/71‑1 (MQ=255)
                                        gagaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTa                            >  1:4662074/1‑71 (MQ=255)
                                         agaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCga                                        >  1:481579/1‑58 (MQ=255)
                                          gaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATc                          >  1:5569807/1‑71 (MQ=255)
                                           aaTAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg                                                         <  1:3102816/39‑1 (MQ=255)
                                            aTAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCgg                        <  1:970128/71‑1 (MQ=255)
                                             tAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCt                                            <  1:791186/50‑1 (MQ=255)
                                             tAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGcc                                >  1:4039750/1‑62 (MQ=255)
                                             tAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGcc                                >  1:2227228/1‑62 (MQ=255)
                                             tAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGc                       >  1:3886279/1‑71 (MQ=255)
                                              aaaGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCg                      >  1:83996/1‑71 (MQ=255)
                                                  gCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGtttt                  <  1:866093/71‑1 (MQ=255)
                                                  gCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGtttt                  <  1:2129364/71‑1 (MQ=255)
                                                   ccAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCg                      >  1:873139/1‑66 (MQ=255)
                                                    cAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc                <  1:2919290/71‑1 (MQ=255)
                                                        gagGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgat                                       <  1:374103/44‑1 (MQ=255)
                                                        gagGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgat                                       <  1:3840922/44‑1 (MQ=255)
                                                        gagGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgat                                       <  1:2432651/44‑1 (MQ=255)
                                                           gTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCtt                                           <  1:2522284/37‑1 (MQ=255)
                                                           gTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCtt                                           <  1:5193216/37‑1 (MQ=255)
                                                           gTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCtt                                           <  1:4915868/37‑1 (MQ=255)
                                                           gTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCtt                                           <  1:4719541/37‑1 (MQ=255)
                                                           gTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCttcttt         <  1:1855415/71‑1 (MQ=255)
                                                             catcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGGTTGCCTCTATCGGCGTTTTCCCttctttct       >  1:5203704/1‑71 (MQ=255)
                                                             catcaGGATTGGGCGTAAACGCATCCGAGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCcttctt          >  1:2094163/1‑68 (MQ=255)
                                                              atcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGttt                   >  1:3396711/1‑58 (MQ=255)
                                                              atcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc                >  1:1203596/1‑61 (MQ=255)
                                                              atcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc                >  1:478239/1‑61 (MQ=255)
                                                                caGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCct              >  1:4589843/1‑61 (MQ=255)
                                                                 aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcat   >  1:5416896/1‑71 (MQ=255)
                                                                 aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcat   >  1:4278776/1‑71 (MQ=255)
                                                                  ggATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcatc  <  1:2337921/71‑1 (MQ=255)
                                                                      |                                                                  
CAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGCATC  >  Exported/2584291‑2584427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: