Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,331 G→A 28.3% L423L (CTA→TTA) ‡ arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,3310GA28.3% 170.3 / 75.1 113L423L (CTA→TTA) ‡arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (38/43);  new base A (17/15);  total (55/58)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCC  >  Exported/2595261‑2595401
                                                                      |                                                                      
cAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAg                                                                        <  1:2406121/71‑1 (MQ=255)
       ggTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgc                                                                 <  1:2453903/71‑1 (MQ=255)
        gTTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgc                                                                 <  1:3826773/70‑1 (MQ=255)
         ttGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgcgc                                                               <  1:340183/71‑1 (MQ=255)
          tgtgATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgcgc                                                               <  1:4702643/70‑1 (MQ=255)
             gATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggc                                                            >  1:4396535/1‑70 (MQ=255)
              aTAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAg                                                                  >  1:3306303/1‑63 (MQ=255)
               tAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagcca                                                          <  1:574231/70‑1 (MQ=255)
                agaTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAg                                                                  <  1:5335149/59‑1 (MQ=255)
                aaaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc                                                        >  1:3469632/1‑71 (MQ=255)
                aaaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc                                                        >  1:3958727/1‑71 (MQ=255)
                aaaTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagccagc                                                        >  1:3462899/1‑71 (MQ=255)
                  aTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGTGCCAGCGCAGCCAGCGt                                                      >  1:4839064/1‑71 (MQ=255)
                  aTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt                                                      >  1:3468783/1‑71 (MQ=255)
                  aTGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGt                                                      <  1:5387995/71‑1 (MQ=255)
                  aTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgcg                                                                >  1:544843/1‑61 (MQ=255)
                  aTGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAgcg                                                                >  1:939264/1‑61 (MQ=255)
                   tGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc                                                     <  1:5228961/71‑1 (MQ=255)
                    ggTAATGTTGCTCATTTCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGtga                                                    >  1:4247100/1‑69 (MQ=255)
                    ggTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGtga                                                    >  1:2836887/1‑69 (MQ=255)
                    ggTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGtga                                                    >  1:4797135/1‑69 (MQ=255)
                         tGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                            <  1:155170/58‑1 (MQ=255)
                          gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                            >  1:2083160/1‑57 (MQ=255)
                          gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                            >  1:4469462/1‑57 (MQ=255)
                          gTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGcagc                                                            >  1:2162843/1‑57 (MQ=255)
                           ttGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGGGCAGCCAGCGTCACAAACAg                                             >  1:5265201/1‑71 (MQ=255)
                           ttGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg                                             >  1:287879/1‑71 (MQ=255)
                           ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                              <  1:3899196/70‑1 (MQ=255)
                           ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                              <  1:4281743/70‑1 (MQ=255)
                           ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                              <  1:5067645/70‑1 (MQ=255)
                           ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa                                              <  1:1086304/70‑1 (MQ=255)
                              cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                          <  1:2572734/71‑1 (MQ=255)
                              cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                          <  1:844464/71‑1 (MQ=255)
                              cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGca                                           <  1:1061871/70‑1 (MQ=255)
                                cATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGcaccg                                        >  1:4169565/1‑69 (MQ=255)
                                 atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                              <  1:2459377/64‑1 (MQ=255)
                                 atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                              <  1:541780/64‑1 (MQ=255)
                                 atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                              <  1:5688686/64‑1 (MQ=255)
                                 atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcagg                                       >  1:4622462/1‑71 (MQ=255)
                                  tatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAgc                                                                 <  1:5650733/44‑1 (MQ=255)
                                  tatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                          <  1:5087646/51‑1 (MQ=255)
                                  tatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                          <  1:4323806/51‑1 (MQ=255)
                                  tatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                          <  1:4603532/51‑1 (MQ=255)
                                   atCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                              >  1:2065172/1‑62 (MQ=255)
                                   atCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggcca                                                          >  1:2786411/1‑50 (MQ=255)
                                    tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc                                                     >  1:2403905/1‑54 (MQ=255)
                                    tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca                                     <  1:1859326/70‑1 (MQ=255)
                                    tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca                                     <  1:1831062/70‑1 (MQ=255)
                                    tCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccaga                                                        >  1:124132/1‑50 (MQ=255)
                                      aGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAggg                                  <  1:977500/71‑1 (MQ=255)
                                        tATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGca                                           <  1:3053832/60‑1 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca                                                  <  1:2242025/51‑1 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg                                             >  1:705235/1‑56 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg                                             >  1:3134278/1‑56 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                                    <  1:3355540/65‑1 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                                    <  1:4290259/65‑1 (MQ=255)
                                          tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                              >  1:65953/1‑71 (MQ=255)
                                          tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGcggccag                                                         <  1:2731350/44‑1 (MQ=12)
                                          tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCAccggcaca                                   >  1:3592595/1‑64 (MQ=255)
                                          tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt                              >  1:1160607/1‑71 (MQ=255)
                                          tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt                              >  1:1518678/1‑71 (MQ=255)
                                             aaTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGa                            >  1:1664135/2‑70 (MQ=255)
                                              aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                           >  1:4377325/1‑69 (MQ=255)
                                              aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                           >  1:1737157/1‑69 (MQ=255)
                                              aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATg                             <  1:2284194/68‑1 (MQ=255)
                                              aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGAt                           <  1:4851837/70‑1 (MQ=255)
                                              aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                          <  1:5540100/71‑1 (MQ=255)
                                               ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa                                              <  1:4860878/50‑1 (MQ=255)
                                               ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa                         <  1:308626/71‑1 (MQ=255)
                                                tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag                                    <  1:5680226/59‑1 (MQ=255)
                                                 aCAAAGTGAAAGAGAGACGTAGCGCCAGCTCAGCCAGCGTCACAAAc                                               >  1:1909327/1‑47 (MQ=255)
                                                 aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                            >  1:4675637/1‑66 (MQ=255)
                                                 aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                       >  1:2094823/1‑71 (MQ=255)
                                                 aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                       >  1:2982070/1‑71 (MQ=255)
                                                  cAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAAtgc                      <  1:3516961/71‑3 (MQ=255)
                                                  cAAAGTGAAAGAGAGACGTAGCGCCAGCTcagcca                                                          >  1:1783513/1‑35 (MQ=255)
                                                  cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                      >  1:1603561/1‑71 (MQ=255)
                                                  cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                      <  1:543292/71‑1 (MQ=255)
                                                   aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                          <  1:1261978/50‑1 (MQ=255)
                                                   aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac                                          <  1:4110322/50‑1 (MQ=255)
                                                    aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg                                   >  1:3923040/1‑56 (MQ=255)
                                                     aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                   <  1:4786190/71‑1 (MQ=255)
                                                     aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                   <  1:4669639/71‑1 (MQ=255)
                                                     aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                   <  1:1826622/71‑1 (MQ=255)
                                                     aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                   <  1:3202795/71‑1 (MQ=255)
                                                      gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                   >  1:5256164/1‑70 (MQ=255)
                                                      gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                   >  1:1153276/1‑70 (MQ=255)
                                                      gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGt                  <  1:969119/71‑1 (MQ=255)
                                                           agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                          >  1:2000060/1‑58 (MQ=255)
                                                              gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg           >  1:1827920/1‑70 (MQ=255)
                                                              gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg           >  1:825321/1‑70 (MQ=255)
                                                              gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt          <  1:5453997/71‑1 (MQ=255)
                                                              gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt          <  1:1696360/71‑1 (MQ=255)
                                                               agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa         <  1:1321587/71‑1 (MQ=255)
                                                                     aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                        >  1:993475/1‑50 (MQ=255)
                                                                     aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                        >  1:1784388/1‑50 (MQ=255)
                                                                     aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                        >  1:5171048/1‑50 (MQ=255)
                                                                      gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                              >  1:83857/1‑43 (MQ=255)
                                                                      gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc  >  1:5591103/1‑71 (MQ=255)
                                                                      gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc  >  1:5127962/1‑71 (MQ=255)
                                                                      |                                                                      
CAGGCCGGGTTGTGATAAATGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCC  >  Exported/2595261‑2595401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: