Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,368 G→A 29.7% T410T (ACC→ACT arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,3680GA29.7% 146.3 / 71.6 101T410T (ACC→ACTarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (39/32);  new base A (17/13);  total (56/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

Only 100 of 115 total aligned reads displayed.

AGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGC  >  Exported/2595299‑2595437
                                                                     |                                                                     
aGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAggg                                                                      <  1:977500/71‑1 (MQ=255)
    tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt                                                                  >  1:1160607/1‑71 (MQ=255)
    tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt                                                                  >  1:1518678/1‑71 (MQ=255)
       aaTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGa                                                                >  1:1664135/2‑70 (MQ=255)
        aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                                                               >  1:4377325/1‑69 (MQ=255)
        aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                                                               >  1:1737157/1‑69 (MQ=255)
        aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac                                                               <  1:3521973/70‑2 (MQ=255)
        aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATg                                                                 <  1:2284194/68‑1 (MQ=255)
        aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGAt                                                               <  1:4851837/70‑1 (MQ=255)
        aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                                                              <  1:5540100/71‑1 (MQ=255)
         ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa                                                             <  1:308626/71‑1 (MQ=255)
          tACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAAt                                                            >  1:5577178/1‑71 (MQ=255)
           aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa                                                                >  1:4675637/1‑66 (MQ=255)
           aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc                                                           >  1:2982070/1‑71 (MQ=255)
            cAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAAtgc                                                          <  1:3516961/71‑3 (MQ=255)
            cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                                                          >  1:1603561/1‑71 (MQ=255)
            cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc                                                          <  1:543292/71‑1 (MQ=255)
              aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg                                                                       >  1:2154353/1‑56 (MQ=255)
              aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg                                                                       >  1:3923040/1‑56 (MQ=255)
               aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                       <  1:3202795/71‑1 (MQ=255)
               aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                       <  1:1826622/71‑1 (MQ=255)
               aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                       <  1:4669639/71‑1 (MQ=255)
               aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                       <  1:4786190/71‑1 (MQ=255)
                gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATgccgg                                                       >  1:5242204/1‑68 (MQ=255)
                gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg                                                       >  1:1153276/1‑70 (MQ=255)
                gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGt                                                      <  1:969119/71‑1 (MQ=255)
                     agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa                                                              >  1:2000060/1‑58 (MQ=255)
                        gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg                                               >  1:825321/1‑70 (MQ=255)
                        gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg                                               >  1:1827920/1‑70 (MQ=255)
                        gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt                                              <  1:5453997/71‑1 (MQ=255)
                        gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt                                              <  1:1696360/71‑1 (MQ=255)
                         agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa                                             <  1:1321587/71‑1 (MQ=255)
                         agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa                                             <  1:5410098/71‑1 (MQ=255)
                               cacgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcc                                       >  1:5377867/3‑71 (MQ=255)
                               aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt                                                            >  1:5171048/1‑50 (MQ=255)
                                gcgcCCGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                                                                  >  1:1979192/1‑43 (MQ=255)
                                gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt                                                                  >  1:83857/1‑43 (MQ=255)
                                gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc                                      >  1:5127962/1‑71 (MQ=255)
                                gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc                                      >  1:5591103/1‑71 (MQ=255)
                                acgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATccc                                      >  1:2590656/2‑71 (MQ=255)
                                acgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATccc                                      >  1:3025161/2‑71 (MQ=255)
                                 cgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc                                     <  1:3471919/71‑1 (MQ=255)
                                      gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc                                     <  1:4704743/66‑1 (MQ=255)
                                      gcgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt                                 >  1:2839713/1‑70 (MQ=255)
                                       cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                               <  1:29933/71‑1 (MQ=255)
                                       cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                               <  1:513441/71‑1 (MQ=255)
                                       cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                               <  1:1243161/71‑1 (MQ=255)
                                         cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCt                                                        <  1:2041747/44‑1 (MQ=255)
                                         cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                               >  1:5070701/1‑69 (MQ=255)
                                          ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg                            >  1:3870623/2‑69 (MQ=255)
                                           gccagaGTGACAAACAGCACCGGCACAGTCATGac                                                               <  1:2147441/35‑2 (MQ=12)
                                           gccagaGTGACAAACAGCACCGGCACAGTCATGac                                                               <  1:584133/35‑2 (MQ=12)
                                           gccagaGTGACAAACAGCACCGGCACAGTCATGac                                                               <  1:257711/35‑2 (MQ=12)
                                             cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGCTAGTCa                         <  1:5555/71‑1 (MQ=255)
                                             cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                         <  1:214073/71‑1 (MQ=255)
                                              agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                         <  1:1674147/70‑1 (MQ=255)
                                              agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                         <  1:396040/70‑1 (MQ=255)
                                              agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                         >  1:5646237/1‑70 (MQ=255)
                                               gcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                       <  1:4893225/71‑1 (MQ=255)
                                                cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                      <  1:1256084/71‑1 (MQ=255)
                                                cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                      <  1:614030/71‑1 (MQ=255)
                                                cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                      <  1:4148128/71‑1 (MQ=255)
                                                 gTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                      <  1:2985680/70‑1 (MQ=255)
                                                  tgacaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTaa                                            >  1:60203/3‑47 (MQ=16)
                                                  tgacaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttt                    <  1:1203956/69‑2 (MQ=255)
                                                  tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                               <  1:4270512/60‑1 (MQ=255)
                                                  tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                               <  1:1595552/60‑1 (MQ=255)
                                                  tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc                    <  1:1791542/71‑1 (MQ=255)
                                                   cacaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc                          >  1:5575767/1‑64 (MQ=255)
                                                    acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt                  <  1:3852905/71‑1 (MQ=255)
                                                    acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttt                   <  1:2152288/70‑3 (MQ=255)
                                                    acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa                                                >  1:2766781/1‑41 (MQ=255)
                                                    acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa                                                >  1:1231302/1‑41 (MQ=255)
                                                    acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa                                                >  1:1390253/1‑41 (MQ=255)
                                                    acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc                                       >  1:24804/1‑50 (MQ=255)
                                                    acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAg                                   >  1:2919375/1‑54 (MQ=255)
                                                    acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                  >  1:4167808/1‑71 (MQ=255)
                                                    acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                  >  1:4352398/1‑71 (MQ=255)
                                                    acaAACAGCACAGGCAGGGTCATGATAATCCCTGAACGGAAGTAATATcc                                       >  1:5395728/1‑50 (MQ=255)
                                                    acaAACAGCAAAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                  >  1:2334813/1‑71 (MQ=255)
                                                     caAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttttc                 <  1:3068514/71‑1 (MQ=255)
                                                      aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct                >  1:1942030/1‑71 (MQ=255)
                                                      aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct                >  1:4119604/1‑71 (MQ=255)
                                                      aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct                >  1:760969/1‑71 (MQ=255)
                                                       aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTg               >  1:1783083/1‑71 (MQ=255)
                                                        aCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGc              >  1:4643370/1‑71 (MQ=255)
                                                          aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg             >  1:74608/1‑68 (MQ=255)
                                                          aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg             >  1:4332141/1‑68 (MQ=255)
                                                             acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATtcttct                >  1:34713/1‑64 (MQ=255)
                                                               aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt                           <  1:2549641/51‑1 (MQ=255)
                                                               aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa       >  1:1731795/1‑71 (MQ=255)
                                                                ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc      <  1:4430395/71‑1 (MQ=255)
                                                                ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc      <  1:1130225/71‑1 (MQ=255)
                                                                 gcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg     <  1:5252685/71‑1 (MQ=255)
                                                                  caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt    <  1:4120927/71‑1 (MQ=255)
                                                                  caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt    <  1:1486229/71‑1 (MQ=255)
                                                                   aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                       >  1:2803758/1‑51 (MQ=255)
                                                                   aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                       >  1:3604778/1‑51 (MQ=255)
                                                                    gggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGc  <  1:1524422/71‑1 (MQ=255)
                                                                     ggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc                    <  1:3415756/52‑1 (MQ=255)
                                                                     |                                                                     
AGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGC  >  Exported/2595299‑2595437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: