Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,405 C→G 28.7% S398T (AGC→ACC) ‡ arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,4050CG28.7% 208.4 / 100.8 139S398T (AGC→ACC) ‡arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base C (52/47);  new base G (18/22);  total (70/69)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

AGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAA  >  Exported/2595336‑2595474
                                                                     |                                                                     
aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt                                                                      <  1:130726/71‑1 (MQ=255)
 gcgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt                                                                      >  1:2839713/1‑70 (MQ=255)
  cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                                                                    <  1:513441/71‑1 (MQ=255)
  cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                                                                    <  1:1243161/71‑1 (MQ=255)
    cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                                                                    >  1:5070701/1‑69 (MQ=255)
     ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg                                                                 >  1:3870623/2‑69 (MQ=255)
        cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                                                              <  1:214073/71‑1 (MQ=255)
         agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                                                              >  1:5646237/1‑70 (MQ=255)
         agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa                                                              <  1:1674147/70‑1 (MQ=255)
          gcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                                                            <  1:4893225/71‑1 (MQ=255)
           cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                                                           <  1:1256084/71‑1 (MQ=255)
           cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                                                           <  1:614030/71‑1 (MQ=255)
           cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                                                           <  1:4148128/71‑1 (MQ=255)
            gTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat                                                           <  1:2985680/70‑1 (MQ=255)
             tgacaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttt                                                         <  1:1203956/69‑2 (MQ=255)
             tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa                                                                    <  1:4270512/60‑1 (MQ=255)
             tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc                                                         <  1:1791542/71‑1 (MQ=255)
               acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt                                                       <  1:3852905/71‑1 (MQ=255)
               acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                                                       >  1:4352398/1‑71 (MQ=255)
                caAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttttc                                                      <  1:3068514/71‑1 (MQ=255)
                caAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttcttc                                                      >  1:2882560/1‑71 (MQ=255)
                 aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct                                                     >  1:760969/1‑71 (MQ=255)
                 aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct                                                     >  1:1942030/1‑71 (MQ=255)
                  aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTg                                                    >  1:1783083/1‑71 (MQ=255)
                    cagcagAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGc                                                   >  1:5555800/1‑70 (MQ=255)
                     aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg                                                  >  1:4332141/1‑68 (MQ=255)
                     aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg                                                  >  1:74608/1‑68 (MQ=255)
                          aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt                                                                <  1:2549641/51‑1 (MQ=255)
                          aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa                                            >  1:1731795/1‑71 (MQ=255)
                           ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc                                           <  1:1130225/71‑1 (MQ=255)
                            gcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg                                          <  1:5252685/71‑1 (MQ=255)
                             caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt                                         <  1:4120927/71‑1 (MQ=255)
                              aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                                                            >  1:2803758/1‑51 (MQ=255)
                              aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                                                            >  1:3604778/1‑51 (MQ=255)
                              acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                        >  1:1389504/4‑71 (MQ=255)
                              acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                        <  1:555069/68‑1 (MQ=255)
                              acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                        <  1:5285263/68‑1 (MQ=255)
                               gggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGc                                       <  1:1524422/71‑1 (MQ=255)
                               cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCa                                                              <  1:3979752/46‑1 (MQ=16)
                               cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                        <  1:2483560/68‑1 (MQ=255)
                                tgTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc                                           <  1:2257996/65‑1 (MQ=255)
                                ggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc                                                         <  1:3415756/52‑1 (MQ=255)
                                  tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGaa                                                <  1:5615276/59‑1 (MQ=255)
                                  tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc                                    >  1:4912920/1‑71 (MQ=255)
                                  tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg                                              >  1:774505/1‑61 (MQ=255)
                                  tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg                                              >  1:4417615/1‑61 (MQ=255)
                                   cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtt                                                          >  1:5272059/1‑48 (MQ=19)
                                   cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGc                                    >  1:3535977/1‑70 (MQ=255)
                                    aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc                                                               >  1:4134779/1‑42 (MQ=255)
                                    aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc                                                               >  1:431553/1‑42 (MQ=255)
                                    aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa                                  <  1:5321996/71‑1 (MQ=255)
                                    aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa                                  <  1:203005/71‑1 (MQ=255)
                                     tGATAATCCCTGTACGGAAGTAATATCCCCAGCTg                                                                     <  1:3026791/35‑1 (MQ=255)
                                     tGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                                                            <  1:3012696/44‑1 (MQ=255)
                                     tGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata                                                            <  1:4453964/44‑1 (MQ=255)
                                       aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                                                       >  1:2099194/1‑47 (MQ=255)
                                       aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt                                                       >  1:4746272/1‑47 (MQ=255)
                                       aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag                               <  1:5564881/71‑1 (MQ=255)
                                        tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt                                                                >  1:700295/1‑37 (MQ=255)
                                        tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                              >  1:4243826/1‑55 (MQ=255)
                                         aaTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTg                                                    >  1:2184508/1‑48 (MQ=19)
                                           tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc                           >  1:4444805/1‑71 (MQ=255)
                                            cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                             >  1:5274802/1‑68 (MQ=255)
                                            cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                             >  1:2801405/1‑68 (MQ=255)
                                              cggTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagtt                        <  1:5530447/69‑2 (MQ=255)
                                               tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                       >  1:3436204/1‑71 (MQ=255)
                                               tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                       >  1:549829/1‑71 (MQ=255)
                                                gTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                      >  1:4700516/1‑71 (MQ=255)
                                                 tACGGAAGTAATATCCCCAGCTGATAGTGATATTCTTCTGCGAAAGTACGTGCAGCCa                                  <  1:5601061/58‑1 (MQ=255)
                                                 tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa                                            >  1:1377180/1‑48 (MQ=255)
                                                 tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa                                            >  1:4353467/1‑48 (MQ=255)
                                                 tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa                                  <  1:1988895/58‑1 (MQ=255)
                                                 tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                       <  1:846704/69‑1 (MQ=255)
                                                  accgAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc                     <  1:4689893/67‑1 (MQ=255)
                                                  aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc                      >  1:4250600/1‑69 (MQ=255)
                                                   cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                   >  1:1359458/1‑71 (MQ=255)
                                                    ggAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                  >  1:2068162/1‑71 (MQ=255)
                                                      aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                 >  1:4607899/1‑70 (MQ=255)
                                                      aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                 >  1:1061447/1‑70 (MQ=255)
                                                      aaGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCac                                 <  1:4735523/54‑2 (MQ=255)
                                                       aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtg               >  1:2813062/1‑70 (MQ=255)
                                                       aGTAATATCCCCAAGTGATTGTCATATTTTTCAGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                <  1:878483/70‑1 (MQ=255)
                                                        gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                        <  1:457075/45‑1 (MQ=255)
                                                         tAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc              >  1:3296041/1‑68 (MQ=255)
                                                          aaTATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCa                                      >  1:3256388/1‑45 (MQ=255)
                                                           atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                  >  1:292915/1‑64 (MQ=255)
                                                           atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa            <  1:5022952/70‑1 (MQ=255)
                                                            tatCCCCAGCTGATAGTCATATTCTTCTGCGaaat                                              <  1:367818/35‑2 (MQ=255)
                                                            tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                              <  1:4743152/35‑1 (MQ=255)
                                                            tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt          <  1:2615888/71‑1 (MQ=255)
                                                             atCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAtt         >  1:2975719/1‑71 (MQ=255)
                                                              tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg        >  1:3338200/1‑71 (MQ=255)
                                                               ccccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTgg       >  1:4586071/1‑71 (MQ=255)
                                                                cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg      >  1:4068552/1‑71 (MQ=255)
                                                                cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg      >  1:1531737/1‑71 (MQ=255)
                                                                  cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                <  1:1476712/43‑1 (MQ=255)
                                                                  cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                <  1:262241/43‑1 (MQ=255)
                                                                  cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                <  1:1632740/43‑1 (MQ=255)
                                                                   aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt    >  1:2497030/1‑70 (MQ=255)
                                                                    gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc                           >  1:1134345/1‑46 (MQ=255)
                                                                     |                                                                     
AGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAA  >  Exported/2595336‑2595474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: