Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,427 A→C 20.8% S391A (TCG→GCG) ‡ arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,4270AC20.8% 203.1 / 69.2 125S391A (TCG→GCG) ‡arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (60/39);  new base C (10/16);  total (70/55)
Fisher's exact test for biased strand distribution p-value = 4.88e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

AGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCA  >  Exported/2595362‑2595497
                                                                 |                                                                      
aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa                                                                   >  1:1731795/1‑71 (MQ=255)
 ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc                                                                  <  1:4430395/71‑1 (MQ=255)
  gcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg                                                                 <  1:5252685/71‑1 (MQ=255)
   caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt                                                                <  1:4120927/71‑1 (MQ=255)
   caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt                                                                <  1:1486229/71‑1 (MQ=255)
    acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCAc                                                                  <  1:1809577/65‑1 (MQ=255)
    acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                               <  1:555069/68‑1 (MQ=255)
    acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                               <  1:5285263/68‑1 (MQ=255)
    acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                               >  1:1389504/4‑71 (MQ=255)
     gggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGc                                                              <  1:1524422/71‑1 (MQ=255)
     cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                               <  1:2483560/68‑1 (MQ=255)
        tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGaa                                                                       <  1:5615276/59‑1 (MQ=255)
        tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg                                                                     >  1:774505/1‑61 (MQ=255)
        tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg                                                                     >  1:4417615/1‑61 (MQ=255)
         cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGc                                                           >  1:3535977/1‑70 (MQ=255)
          aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa                                                         <  1:5321996/71‑1 (MQ=255)
          aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                               >  1:833614/1‑65 (MQ=255)
          aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa                                                         <  1:203005/71‑1 (MQ=255)
             aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag                                                      <  1:5564881/71‑1 (MQ=255)
              tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                                                     >  1:5424356/1‑55 (MQ=255)
                 tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc                                                  >  1:4444805/1‑71 (MQ=255)
                  cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                    >  1:516239/1‑68 (MQ=255)
                  cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                    >  1:4924942/1‑68 (MQ=255)
                  cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                    >  1:5274802/1‑68 (MQ=255)
                  cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta                                                    >  1:2801405/1‑68 (MQ=255)
                   ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg                                                 >  1:4551306/1‑70 (MQ=255)
                    cggTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagtt                                               <  1:5530447/69‑2 (MQ=255)
                     tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                              >  1:3436204/1‑71 (MQ=255)
                      gTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                                             >  1:4700516/1‑71 (MQ=255)
                      gTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCAcat                                                                <  1:2890320/52‑3 (MQ=255)
                       tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa                                                                   >  1:1377180/1‑48 (MQ=255)
                       tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag                                              <  1:846704/69‑1 (MQ=255)
                        accgAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGcc                                            <  1:4689893/67‑1 (MQ=255)
                        aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc                                             >  1:4250600/1‑69 (MQ=255)
                          ggAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                                         >  1:2068162/1‑71 (MQ=255)
                            aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                        >  1:4607899/1‑70 (MQ=255)
                            aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                        >  1:1061447/1‑70 (MQ=255)
                            aaGTAATATCCCCAAGTGATTGTCATATTTTTCTTGGCAAGCACATGCAGCCac                                                        <  1:3114598/54‑2 (MQ=255)
                            aaGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCac                                                        <  1:4735523/54‑2 (MQ=255)
                             aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtg                                      >  1:2813062/1‑70 (MQ=255)
                             aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                                       <  1:1140045/70‑1 (MQ=255)
                             aGTAATATCCCCAAGTGATTGTCATATTTTTCAGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                                       <  1:878483/70‑1 (MQ=255)
                              gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                               <  1:457075/45‑1 (MQ=255)
                              gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                                               <  1:1365411/45‑1 (MQ=255)
                              gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                                     <  1:1763288/71‑3 (MQ=255)
                               tAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc                                     >  1:3296041/1‑68 (MQ=255)
                                aaTATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCa                                                             >  1:3256388/1‑45 (MQ=255)
                                 atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg                                         >  1:292915/1‑64 (MQ=255)
                                 atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg                                          >  1:5683652/1‑63 (MQ=255)
                                 atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa                                  >  1:2033974/1‑71 (MQ=255)
                                 atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa                                   <  1:5022952/70‑1 (MQ=255)
                                  tatCCCCAGCTGATAGTCATATTCTTCTGCGaaat                                                                     <  1:367818/35‑2 (MQ=255)
                                  tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAg                                                                     <  1:4743152/35‑1 (MQ=255)
                                  tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCATAGTAGCGTAGCCAGGCTACCaa                                  <  1:4347204/70‑1 (MQ=255)
                                  tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                                 <  1:2615888/71‑1 (MQ=255)
                                    tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg                               >  1:3338200/1‑71 (MQ=255)
                                      cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                             >  1:4068552/1‑71 (MQ=255)
                                      cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg                             >  1:1531737/1‑71 (MQ=255)
                                        cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                       <  1:1632740/43‑1 (MQ=255)
                                        cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                       <  1:1476712/43‑1 (MQ=255)
                                        cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga                                                       <  1:262241/43‑1 (MQ=255)
                                         aGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGt                           >  1:2497030/1‑70 (MQ=255)
                                          gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc                                                  >  1:1134345/1‑46 (MQ=255)
                                          gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                         <  1:1272417/71‑1 (MQ=255)
                                            tGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCTAGGCTACCa                                   <  1:2456311/59‑1 (MQ=255)
                                              aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc                                             >  1:124800/1‑47 (MQ=255)
                                              aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAtt                       <  1:4232981/69‑1 (MQ=255)
                                               tAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag                                                      <  1:1191807/37‑1 (MQ=255)
                                                aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                                               >  1:1767616/1‑43 (MQ=255)
                                                aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgta                                               >  1:2896609/1‑43 (MQ=255)
                                                aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt                                 >  1:4653318/1‑57 (MQ=255)
                                                aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                   >  1:3891123/1‑71 (MQ=255)
                                                aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                   >  1:3583917/1‑71 (MQ=255)
                                                 gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                   <  1:5434796/70‑1 (MQ=255)
                                                  tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCggg                 <  1:1234240/71‑2 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                           >  1:1007220/1‑43 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                           >  1:3477133/1‑43 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAttt                      >  1:979338/1‑64 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                    >  1:61438/1‑66 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                    >  1:2489482/1‑66 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                    >  1:866379/1‑66 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc               >  1:3251462/1‑71 (MQ=255)
                                                    atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc               >  1:4548297/1‑71 (MQ=255)
                                                     tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc               >  1:4974575/1‑70 (MQ=255)
                                                       ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa            <  1:4267265/71‑1 (MQ=255)
                                                        tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCa                                           <  1:4705840/39‑1 (MQ=255)
                                                        tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa                                   <  1:1290473/47‑1 (MQ=255)
                                                        tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc              >  1:1699749/1‑68 (MQ=255)
                                                         cttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaaa           <  1:4285372/70‑1 (MQ=255)
                                                          ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                        <  1:1002196/40‑1 (MQ=255)
                                                          ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc                                        >  1:1011515/1‑40 (MQ=255)
                                                          ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc         >  1:696216/1‑71 (MQ=255)
                                                              gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg     >  1:4940678/1‑71 (MQ=255)
                                                              gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg     >  1:2687148/1‑71 (MQ=255)
                                                              gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg     >  1:3120286/1‑71 (MQ=255)
                                                               cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc       <  1:2793191/68‑1 (MQ=255)
                                                               cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc       <  1:2964023/68‑1 (MQ=255)
                                                                gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc         <  1:3178037/65‑1 (MQ=255)
                                                                gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc    >  1:3121949/1‑70 (MQ=255)
                                                                 aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa  <  1:1807966/71‑1 (MQ=255)
                                                                 |                                                                      
AGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCA  >  Exported/2595362‑2595497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: