Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,473 A→G 19.7% I375I (ATT→ATC arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,4730AG19.7% 268.3 / 58.1 132I375I (ATT→ATCarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base A (54/52);  new base G (16/10);  total (70/62)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 137 total aligned reads displayed.

GCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCG  >  Exported/2595404‑2595543
                                                                     |                                                                      
gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa                                                                       <  1:1272417/71‑1 (MQ=255)
    aTAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAtt                                                                     <  1:4232981/69‑1 (MQ=255)
      aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                                 >  1:3891123/1‑71 (MQ=255)
      aGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                                 >  1:3583917/1‑71 (MQ=255)
       gTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                                 <  1:5434796/70‑1 (MQ=255)
        tCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCggg                                                               <  1:1234240/71‑2 (MQ=255)
          atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                                  >  1:3968983/1‑66 (MQ=255)
          atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                                  >  1:61438/1‑66 (MQ=255)
          atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTc                                                                  >  1:2489482/1‑66 (MQ=255)
          atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                                             >  1:3141656/1‑71 (MQ=255)
          atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                                             >  1:3251462/1‑71 (MQ=255)
          atatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                                             >  1:4548297/1‑71 (MQ=255)
           tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                                             >  1:3336725/1‑70 (MQ=255)
           tatTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTcc                                                             >  1:4974575/1‑70 (MQ=255)
             ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa                                                          <  1:4267265/71‑1 (MQ=255)
             ttcttcTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCaa                                                          <  1:5141489/71‑1 (MQ=255)
              tcttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTccc                                                            >  1:1699749/1‑68 (MQ=255)
                ttctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                                                       >  1:696216/1‑71 (MQ=255)
                 tctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg                                                      >  1:855357/1‑71 (MQ=255)
                    gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                                                   >  1:2687148/1‑71 (MQ=255)
                    gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg                                                   >  1:4940678/1‑71 (MQ=255)
                     cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc                                                     <  1:2793191/68‑1 (MQ=255)
                      gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATc                                                       <  1:3178037/65‑1 (MQ=255)
                      gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc                                                  >  1:3121949/1‑70 (MQ=255)
                       aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa                                                <  1:1807966/71‑1 (MQ=255)
                        aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCgg                                                                >  1:1273099/1‑54 (MQ=255)
                        aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCg                                                                 >  1:3799103/1‑53 (MQ=255)
                        aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                                               <  1:4062936/71‑1 (MQ=255)
                        aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa                                               <  1:1582911/71‑1 (MQ=255)
                        aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACAAATTGGGGTAATTTTc                                                                  >  1:1483323/1‑52 (MQ=255)
                         aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAAt                                              >  1:3615097/1‑71 (MQ=255)
                           tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAAt                                                                      <  1:5153649/45‑1 (MQ=255)
                           tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca                                            >  1:3863892/1‑71 (MQ=255)
                           tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca                                            <  1:2751941/71‑1 (MQ=255)
                            acatGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATca                                            >  1:1514912/4‑70 (MQ=255)
                              gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt                                         <  1:3790476/71‑1 (MQ=255)
                              gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt                                         <  1:4778729/71‑1 (MQ=255)
                              atGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACAt                                         <  1:3814260/70‑1 (MQ=255)
                                     cAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                                  <  1:1972875/71‑1 (MQ=255)
                                      agagTAGCGTAGCCAGGCTACCAATTGGGGTAAtttt                                                                   <  1:3498099/37‑1 (MQ=255)
                                      agagTAGCGTAGCCAGGCTACCAATTGGGGTAAttt                                                                    >  1:3833663/1‑36 (MQ=255)
                                      agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                                  >  1:2716251/1‑70 (MQ=255)
                                         gtagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc                               >  1:5664621/1‑70 (MQ=255)
                                          tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                                                           <  1:892936/41‑1 (MQ=255)
                                          tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                                                           <  1:507659/41‑1 (MQ=255)
                                          tagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCa                                                           <  1:698790/41‑1 (MQ=255)
                                               ttgcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCAt                            >  1:308917/3‑67 (MQ=255)
                                               ttgcCAGACTGCCAACCGGGGTGATTTTCGGGCCTAAATCACAGCCAATcac                                           <  1:5169098/50‑1 (MQ=255)
                                               tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcac                                           <  1:3109399/52‑1 (MQ=255)
                                               tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCAt                            <  1:2479251/67‑1 (MQ=255)
                                               tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCAt                            <  1:5261264/67‑1 (MQ=255)
                                                agcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGct                        >  1:117541/1‑70 (MQ=255)
                                                agcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAACCGCAGCCAATCACATTGGCATAAACCATCGctt                       >  1:1749143/1‑71 (MQ=255)
                                                 gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTg                                       >  1:830136/1‑54 (MQ=255)
                                                 gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctc                      >  1:4111743/1‑71 (MQ=255)
                                                 gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctc                      >  1:138197/1‑71 (MQ=255)
                                                    aGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCttt                   <  1:1136169/71‑1 (MQ=255)
                                                      gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                                  <  1:5621723/54‑1 (MQ=255)
                                                      gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa                                  <  1:5274453/54‑1 (MQ=255)
                                                      gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa                 >  1:44154/1‑71 (MQ=255)
                                                      gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa                 >  1:3076353/1‑71 (MQ=255)
                                                       ctgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctct                     >  1:3569147/4‑66 (MQ=255)
                                                       ctgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctct                     >  1:445603/4‑66 (MQ=255)
                                                       ctgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctct                     >  1:5456389/4‑66 (MQ=255)
                                                       ctgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctct                     >  1:2284202/4‑66 (MQ=255)
                                                       cTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca                                            <  1:2849464/43‑1 (MQ=255)
                                                        tgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATca                                            <  1:5516054/40‑1 (MQ=16)
                                                        tgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATca                                            <  1:4793352/40‑1 (MQ=16)
                                                        tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCg                          >  1:4759544/1‑60 (MQ=255)
                                                        tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt                >  1:2367820/1‑70 (MQ=255)
                                                        tACCAATTGGGGTAATTTTAGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt                >  1:3327418/1‑70 (MQ=255)
                                                          ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATc                                             >  1:1156533/1‑39 (MQ=255)
                                                          ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATc                           <  1:4385994/57‑1 (MQ=255)
                                                          ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAc             >  1:3451052/1‑71 (MQ=255)
                                                          ccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGacg               <  1:530882/69‑3 (MQ=255)
                                                           cAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca                                          <  1:2562623/41‑1 (MQ=255)
                                                           cAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca                                          <  1:5446397/41‑1 (MQ=255)
                                                            aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCa                             >  1:1377362/1‑53 (MQ=255)
                                                            aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc           <  1:2905130/71‑1 (MQ=255)
                                                             atcggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc           <  1:97747/67‑1 (MQ=255)
                                                              ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAcc                              <  1:5342575/50‑1 (MQ=255)
                                                              ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc           >  1:3748577/1‑69 (MQ=255)
                                                              ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc           >  1:3177689/1‑69 (MQ=255)
                                                              tcggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCa                             >  1:2381800/3‑51 (MQ=255)
                                                               tGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc           >  1:4094631/1‑68 (MQ=255)
                                                                ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc                               <  1:4203771/47‑1 (MQ=255)
                                                                ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc                               <  1:2011736/47‑1 (MQ=255)
                                                                ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa              >  1:1869377/1‑64 (MQ=255)
                                                                ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa              >  1:2880212/1‑64 (MQ=255)
                                                                ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa              >  1:623043/1‑64 (MQ=255)
                                                                ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc           >  1:4738066/1‑67 (MQ=255)
                                                                 gggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgtc      >  1:3301054/1‑69 (MQ=255)
                                                                 gggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGa       <  1:2410889/70‑1 (MQ=255)
                                                                 gggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGa       <  1:720008/70‑1 (MQ=255)
                                                                  ggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt                <  1:1616517/60‑1 (MQ=255)
                                                                   gTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCa                             <  1:5212239/46‑1 (MQ=255)
                                                                   gTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTg                  <  1:4444327/57‑1 (MQ=255)
                                                                    tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc   <  1:848510/71‑1 (MQ=255)
                                                                    tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc   >  1:4639318/1‑71 (MQ=255)
                                                                     aaTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg  >  1:1316500/1‑71 (MQ=255)
                                                                     |                                                                      
GCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCG  >  Exported/2595404‑2595543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: