Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,566 G→A 16.8% G344G (GGC→GGT arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,5660GA16.8% 244.2 / 35.4 107G344G (GGC→GGTarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (44/45);  new base A (8/10);  total (52/55)
Fisher's exact test for biased strand distribution p-value = 7.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

ATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCG  >  Exported/2595498‑2595632
                                                                    |                                                                  
aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc                                                                  <  1:5071896/71‑1 (MQ=255)
aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc                                                                  <  1:2079645/71‑1 (MQ=255)
 tCACATTGGCATAAATCATTGCCTCTTTGACGCCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCAcc                                                                  >  1:2467144/1‑70 (MQ=255)
 tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCa                                                                 <  1:2781269/71‑1 (MQ=255)
  cacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa                                                                >  1:464410/1‑71 (MQ=255)
   acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa                                                                <  1:466127/70‑1 (MQ=255)
   acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa                                                                >  1:3466013/1‑70 (MQ=255)
   acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                               <  1:1037860/71‑1 (MQ=255)
   acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                               >  1:2308503/1‑71 (MQ=255)
   acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                               >  1:3459158/1‑71 (MQ=255)
   acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                               >  1:157761/1‑71 (MQ=255)
   acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                               <  1:3254161/71‑1 (MQ=255)
    caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc                                                              <  1:1634087/71‑1 (MQ=255)
    caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc                                                               <  1:1966156/70‑1 (MQ=255)
     aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                                             <  1:4849085/71‑1 (MQ=255)
      ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg                                                            <  1:1979696/71‑1 (MQ=255)
       tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa                                                                >  1:3055542/1‑66 (MQ=255)
       tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa                                                                >  1:3920017/1‑66 (MQ=255)
        ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt                                                           <  1:1472548/70‑1 (MQ=255)
        ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt                                                           <  1:4262823/70‑1 (MQ=255)
         gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa                                                          <  1:3422995/70‑1 (MQ=255)
         gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa                                                          <  1:2396974/70‑1 (MQ=255)
            tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                                                      >  1:1124572/1‑71 (MQ=255)
             aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc                                                         >  1:1249439/1‑67 (MQ=255)
             aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                     >  1:2560031/1‑71 (MQ=255)
              aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                                                                    >  1:5035245/1‑55 (MQ=255)
              aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                                                                    >  1:2718291/1‑55 (MQ=255)
              aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                                                                    >  1:3736814/1‑55 (MQ=255)
              aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                                                                    >  1:611440/1‑55 (MQ=255)
              aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                     <  1:4005571/70‑1 (MQ=255)
              aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                    >  1:1911825/1‑71 (MQ=255)
              aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                    >  1:190643/1‑71 (MQ=255)
              aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                    >  1:4762948/1‑71 (MQ=255)
              aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg                                                    >  1:4005558/1‑71 (MQ=255)
              aaCCATCGCTTCTTTGATAACGCCAGATGCCGAGCTGCCATCAATGGACAACGCGCCAAcc                                                              >  1:4243777/1‑61 (MQ=255)
              aaCCATAGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg                                                                    >  1:3215243/1‑55 (MQ=255)
                     gcctcttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCatat                                              >  1:300896/4‑70 (MQ=255)
                     gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt                                             >  1:285528/1‑71 (MQ=255)
                      cctcttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg                                            <  1:4234961/69‑1 (MQ=255)
                         cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                                           <  1:1743118/69‑1 (MQ=255)
                           ttGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                                                   >  1:1388473/1‑43 (MQ=255)
                           ttGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc                                                                   >  1:2577139/1‑43 (MQ=255)
                            tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt                                                      >  1:389031/1‑55 (MQ=255)
                             gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                     >  1:467426/1‑71 (MQ=255)
                              aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt                                           >  1:5610185/1‑64 (MQ=255)
                               cgaCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTc                                         <  1:2852098/63‑1 (MQ=255)
                                 aCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                                       <  1:5301797/65‑1 (MQ=255)
                                 aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATaga                                 <  1:1448564/71‑1 (MQ=255)
                                 aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATaga                                 <  1:1441744/71‑1 (MQ=255)
                                 aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                  <  1:1061604/70‑1 (MQ=255)
                                 aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                  <  1:4980414/70‑1 (MQ=255)
                                 aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                  <  1:364533/70‑1 (MQ=255)
                                 aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                  <  1:5685742/70‑1 (MQ=255)
                                  cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                                <  1:464555/71‑1 (MQ=255)
                                  cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa                                <  1:3890566/71‑1 (MQ=255)
                                   gCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCatca                                     >  1:1893540/1‑63 (MQ=255)
                                   gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                                             >  1:2674055/1‑41 (MQ=255)
                                   gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa                                                             >  1:2004573/1‑41 (MQ=255)
                                   gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaag                               <  1:1003764/71‑1 (MQ=255)
                                    ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAcc                                                        <  1:2483012/45‑1 (MQ=255)
                                    ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                              <  1:1625058/71‑1 (MQ=255)
                                       gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag                           <  1:5283863/71‑1 (MQ=255)
                                          gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                     >  1:1026562/1‑42 (MQ=255)
                                          gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                     >  1:5695721/1‑42 (MQ=255)
                                          gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                     >  1:5313032/1‑42 (MQ=255)
                                          gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc                                                     >  1:1142167/1‑42 (MQ=255)
                                          gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg                                            >  1:3789575/1‑51 (MQ=255)
                                          gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGt                                           >  1:2675742/1‑52 (MQ=255)
                                           ccGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGtt                                          >  1:4158798/1‑52 (MQ=255)
                                           ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                                       <  1:1134433/55‑1 (MQ=255)
                                           ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                                       <  1:5532385/55‑1 (MQ=255)
                                           ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                                       <  1:4648604/55‑1 (MQ=255)
                                            cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg                      >  1:4332174/1‑69 (MQ=255)
                                              tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCgg                                                   <  1:3779870/40‑1 (MQ=255)
                                              tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCgg                                                   <  1:2187126/40‑1 (MQ=255)
                                              tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCg                    <  1:2098620/71‑1 (MQ=255)
                                                   acATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAg               >  1:2566778/2‑71 (MQ=255)
                                                    cgtCAATCGACAGCGCACCAATCAGTACCGTCGGCATATTGTTCat                                       >  1:1992271/3‑46 (MQ=25)
                                                    cgtCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat                                       >  1:566624/3‑46 (MQ=255)
                                                    cATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa                                     >  1:3032727/1‑48 (MQ=255)
                                                      tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg                                            <  1:1539955/39‑1 (MQ=255)
                                                       cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt           >  1:606189/1‑71 (MQ=255)
                                                        aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                      >  1:2973408/1‑43 (MQ=255)
                                                        aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata                                      >  1:1108406/1‑43 (MQ=255)
                                                        aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag                             >  1:2789313/1‑52 (MQ=255)
                                                        aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc          <  1:4929070/71‑3 (MQ=255)
                                                        aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc          <  1:3490046/71‑3 (MQ=255)
                                                         aTGGACAACCCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc          >  1:3660895/1‑70 (MQ=255)
                                                          tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                  <  1:3637119/45‑1 (MQ=255)
                                                          tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                  <  1:3300775/45‑1 (MQ=255)
                                                          tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag                                  <  1:4363712/45‑1 (MQ=255)
                                                          tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt                  <  1:218872/61‑1 (MQ=255)
                                                            gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa                        >  1:1057806/1‑53 (MQ=255)
                                                              cAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc    >  1:3578976/1‑71 (MQ=255)
                                                                gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg  <  1:3499369/70‑1 (MQ=255)
                                                                gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg  <  1:4148179/70‑1 (MQ=255)
                                                                 cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                              >  1:2020014/1‑42 (MQ=255)
                                                                 cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                              >  1:5598692/1‑42 (MQ=255)
                                                                 cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga                              >  1:800238/1‑42 (MQ=255)
                                                                 cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg       >  1:1755250/1‑65 (MQ=255)
                                                                    |                                                                  
ATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCG  >  Exported/2595498‑2595632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: