| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 312,529 | A→G | 17.7% | M115T (ATG→ACG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 312,529 | 0 | A | G | 17.7% | 263.6 / 42.6 | 113 | M115T (ATG→ACG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (53/40); new base G (9/11); total (62/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
CATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAG > Exported/312459‑312599 | cATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCa > 1:4614853/1‑71 (MQ=255)cATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCa > 1:4944481/1‑71 (MQ=255) aTCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCAt < 1:4390367/71‑1 (MQ=255) aTCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCAt > 1:1760925/1‑71 (MQ=255) aGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCg < 1:1136844/70‑1 (MQ=255) ggAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGcc < 1:1255017/71‑1 (MQ=255) gAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCg < 1:4304936/68‑1 (MQ=255) gAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCa < 1:4812912/71‑1 (MQ=255) ctgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATc > 1:899414/1‑60 (MQ=255) ctgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATc > 1:4102938/1‑60 (MQ=255) tgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt > 1:3063409/1‑71 (MQ=255) tgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt > 1:563107/1‑71 (MQ=255) ggATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAg < 1:1328137/69‑1 (MQ=255) ggATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGtt > 1:2160592/1‑71 (MQ=255) gatgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGttt > 1:1003350/1‑71 (MQ=255) tgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGt < 1:2535880/71‑1 (MQ=255) gatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGtt > 1:2136240/1‑71 (MQ=255) atTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCt > 1:4721322/1‑60 (MQ=255) atTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTc > 1:3648995/1‑71 (MQ=255) tttCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCt > 1:2938909/1‑71 (MQ=255) tctcAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTg > 1:4675381/1‑70 (MQ=255) tctcAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTg > 1:1653928/1‑70 (MQ=255) ttcAACACTGATCCCCTGCTGCTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCa > 1:3473372/2‑71 (MQ=255) ttcAACACTGATCCCCTGCTGCTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCa > 1:2213787/2‑71 (MQ=255) ctcAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGc < 1:2942190/70‑1 (MQ=255) cGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTg < 1:381890/70‑1 (MQ=255) cGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGa < 1:4243671/71‑1 (MQ=255) cGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGa < 1:1460206/71‑1 (MQ=255) cGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGa < 1:5650579/71‑1 (MQ=255) gCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGaa > 1:2302376/1‑71 (MQ=255) cTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt < 1:5464836/54‑1 (MQ=255) cTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc > 1:5188030/1‑71 (MQ=255) cTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc > 1:4415100/1‑71 (MQ=255) tCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAcc > 1:2304822/1‑71 (MQ=255) aCCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCt < 1:1199429/70‑1 (MQ=255) aCCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTg > 1:124613/1‑71 (MQ=255) aCCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTg > 1:444578/1‑71 (MQ=255) cccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTg < 1:195482/52‑1 (MQ=255) cccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTg < 1:1997438/52‑1 (MQ=255) cccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcg > 1:3268437/1‑71 (MQ=255) cccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcg > 1:5373523/1‑71 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcgc > 1:3131442/1‑71 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcgc > 1:1201616/1‑71 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcgc > 1:1830243/1‑71 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcg > 1:630402/1‑70 (MQ=255) cTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCAcc > 1:2674422/1‑59 (MQ=255) ttGCTGCTCAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGa < 1:2550526/61‑1 (MQ=255) tgctgcTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTg < 1:914114/41‑1 (MQ=19) tgctgcTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTg < 1:238437/41‑1 (MQ=19) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCt > 1:442104/1‑57 (MQ=255) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGata > 1:3822746/1‑71 (MQ=255) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGata > 1:5065724/1‑71 (MQ=255) gctgctGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGtt < 1:2431612/45‑1 (MQ=16) gctgctGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTc > 1:3610013/1‑50 (MQ=19) gctgctGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTc > 1:3609236/1‑50 (MQ=19) gctgctGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTc > 1:3360919/1‑50 (MQ=19) ctgctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGatat > 1:3947693/1‑71 (MQ=255) gctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt < 1:3670099/41‑1 (MQ=255) gctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt < 1:4092937/41‑1 (MQ=255) gctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt < 1:4430184/41‑1 (MQ=255) gctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc < 1:4098675/58‑1 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:448469/1‑70 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:515958/1‑70 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:4642006/1‑70 (MQ=255) cttcttGCGGCTGCAACGGCATCGCCAGCTGCAGTTTGTTCTGc < 1:277137/44‑1 (MQ=255) cttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGc < 1:3342115/44‑1 (MQ=255) cttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCt > 1:2598920/1‑54 (MQ=255) ttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGa < 1:2553563/49‑1 (MQ=255) tcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc > 1:1260472/1‑50 (MQ=255) tcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc > 1:1774050/1‑50 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg < 1:4296053/69‑1 (MQ=255) ttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:5200020/1‑71 (MQ=255) ctGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGt < 1:3209470/34‑1 (MQ=12) ctGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGt < 1:2746399/34‑1 (MQ=12) tGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:4264842/1‑70 (MQ=255) gCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGtc < 1:870112/68‑2 (MQ=16) gCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGtc < 1:2828131/68‑2 (MQ=16) gCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg > 1:3609367/1‑70 (MQ=16) gCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg > 1:233171/1‑70 (MQ=16) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTc < 1:510094/35‑1 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTa < 1:4587399/68‑1 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:4298404/1‑69 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:2560355/1‑69 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc < 1:5343728/69‑1 (MQ=255) cGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCa < 1:3411071/70‑1 (MQ=255) agcaACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtg < 1:4679745/71‑2 (MQ=16) agcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGa < 1:409901/68‑1 (MQ=255) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:4628002/71‑1 (MQ=255) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:3845462/71‑1 (MQ=255) caACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtgga < 1:3355794/71‑4 (MQ=12) caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGa < 1:3765029/66‑1 (MQ=255) caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaa < 1:1095740/71‑1 (MQ=255) aaCGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtgg > 1:4200181/1‑67 (MQ=12) aaCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaa < 1:3928987/70‑1 (MQ=255) aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:1332078/1‑71 (MQ=255) cGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAgg > 1:5432914/1‑71 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:3842056/1‑69 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt > 1:1006632/1‑71 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:1776993/71‑1 (MQ=255) gCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTc < 1:480879/71‑1 (MQ=255) | CATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAG > Exported/312459‑312599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |