Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,701 G→T 11.1% A299A (GCC→GCA arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,7010GT11.1% 288.8 / 13.0 94A299A (GCC→GCAarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (50/33);  new base T (4/6);  total (55/39)
Fisher's exact test for biased strand distribution p-value = 3.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 101 total aligned reads displayed.

TCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGC  >  Exported/2595637‑2595766
                                                                |                                                                 
tCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                                  <  1:2943725/66‑1 (MQ=255)
  gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                           >  1:981857/1‑71 (MQ=255)
  gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                           >  1:3402723/1‑71 (MQ=255)
  gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                           >  1:1999490/1‑71 (MQ=255)
   cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcgc                                                          <  1:3485629/71‑1 (MQ=255)
    ggCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                           >  1:3567205/1‑69 (MQ=255)
     gCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCAtt                                                              <  1:2292966/65‑1 (MQ=255)
       ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgc                                                            >  1:3961938/1‑65 (MQ=255)
       ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                                      >  1:3780043/1‑71 (MQ=255)
       ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                                      >  1:5602778/1‑71 (MQ=255)
       ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                                      >  1:2526325/1‑71 (MQ=255)
        cacaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc                                                      >  1:4903128/1‑70 (MQ=255)
         acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                                    >  1:4029242/1‑71 (MQ=255)
         acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                                    >  1:1896900/1‑71 (MQ=255)
         acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                                    >  1:4126379/1‑71 (MQ=255)
         acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                                    >  1:2875794/1‑71 (MQ=255)
         acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa                                                    >  1:2682705/1‑71 (MQ=255)
               cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                              <  1:5526257/71‑1 (MQ=255)
                 ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                                  <  1:127529/49‑1 (MQ=255)
                 ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                                  <  1:5383454/49‑1 (MQ=255)
                 ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                                  <  1:2278032/49‑1 (MQ=255)
                 ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                                  <  1:5618776/49‑1 (MQ=255)
                 ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa                                                <  1:4828598/67‑1 (MQ=255)
                  tATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                            <  1:2842828/70‑1 (MQ=255)
                   aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCa                                                                >  1:4861178/1‑49 (MQ=255)
                   aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCa                                                                >  1:1257627/1‑49 (MQ=255)
                   aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAgg                                                       >  1:2411165/1‑58 (MQ=255)
                   aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                            >  1:4305840/1‑69 (MQ=255)
                   aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                            >  1:2871876/1‑69 (MQ=255)
                   aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                            >  1:1762316/1‑69 (MQ=255)
                   aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                                             >  1:5442086/1‑68 (MQ=255)
                   aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                                             >  1:2537540/1‑68 (MQ=255)
                   aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca                                             >  1:1326877/1‑68 (MQ=255)
                   aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                              >  1:2400230/1‑67 (MQ=255)
                   aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc                                              >  1:4164990/1‑67 (MQ=255)
                   aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                                               >  1:1798524/1‑66 (MQ=255)
                     ctgCCAGCAGATTCAGCACGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATa                                                  >  1:5314827/3‑61 (MQ=255)
                     ctgCCAGCAGATTCAGCACGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATa                                                  >  1:3648782/3‑61 (MQ=255)
                      cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                        <  1:2578486/70‑1 (MQ=255)
                      cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                        <  1:5487768/70‑1 (MQ=255)
                      cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa                                        <  1:237230/70‑1 (MQ=255)
                      cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                                       <  1:394464/71‑1 (MQ=255)
                      cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt                                       <  1:4913772/71‑1 (MQ=255)
                        ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATa                                      <  1:5692575/70‑1 (MQ=255)
                          aGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACAt                                   >  1:619326/1‑71 (MQ=255)
                              cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                               <  1:2684351/71‑1 (MQ=255)
                              cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg                               <  1:4507846/71‑1 (MQ=255)
                               gTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                                  <  1:913053/35‑1 (MQ=255)
                               gTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg                                                                  <  1:2980581/35‑1 (MQ=255)
                                ttGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATa                                                  <  1:2925855/50‑1 (MQ=255)
                                 tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca                                          >  1:1491589/1‑57 (MQ=255)
                                 tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc                            >  1:1370406/1‑71 (MQ=255)
                                  gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                           >  1:53938/1‑39 (MQ=255)
                                  gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg                                                           >  1:564529/1‑39 (MQ=255)
                                  gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                                               >  1:962069/1‑51 (MQ=255)
                                  gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac                                               >  1:5452169/1‑51 (MQ=255)
                                      aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa                       <  1:3268683/71‑1 (MQ=255)
                                      aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa                       <  1:434414/71‑1 (MQ=255)
                                      aCGCCAGAAAGATATTCCTTTAATCCAGCATTGCGCAGGCCATaaa                                                >  1:4252219/1‑46 (MQ=255)
                                      aCGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa                                                >  1:3645637/1‑46 (MQ=255)
                                      aCGACAGAAAGATATTCCGTTAATCCGGCATTGAGCAGGCCATaaa                                                >  1:545180/1‑46 (MQ=39)
                                       cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaa                                                 >  1:40940/1‑44 (MQ=255)
                                       cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag                         >  1:3286343/1‑68 (MQ=255)
                                        gCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaaaa                     <  1:951590/71‑3 (MQ=255)
                                         ccAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                    <  1:328670/71‑1 (MQ=255)
                                         ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                            >  1:1359151/1‑47 (MQ=255)
                                         ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac                                            >  1:3714069/1‑47 (MQ=255)
                                         ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                    <  1:5096957/71‑1 (MQ=255)
                                         ccAGAAAGATATTCCGTTAATCCGGAATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga                     >  1:4739359/1‑70 (MQ=255)
                                          cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                    >  1:4179180/1‑70 (MQ=255)
                                          cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt                    <  1:3390875/70‑1 (MQ=255)
                                           aGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa                  <  1:3853428/71‑1 (MQ=255)
                                            gAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaa                                                 <  1:885608/39‑1 (MQ=255)
                                             aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                       >  1:3888093/1‑64 (MQ=255)
                                             aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                       >  1:1902425/1‑64 (MQ=255)
                                             aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa                       >  1:5593260/1‑64 (MQ=255)
                                               aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt              >  1:5290268/1‑71 (MQ=255)
                                                 atatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag                      <  1:3366957/61‑1 (MQ=255)
                                                   atTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt                    >  1:4202109/1‑61 (MQ=255)
                                                    ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc                            <  1:343597/52‑1 (MQ=255)
                                                    ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc         <  1:5425102/71‑1 (MQ=255)
                                                    ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc         <  1:2489466/71‑1 (MQ=255)
                                                    ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc          <  1:3432388/70‑1 (MQ=255)
                                                    ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc          <  1:639752/70‑1 (MQ=255)
                                                     tCCGTTAATCCGGCATTGCGCAGGCCATAAaccacc                                           >  1:1406758/1‑36 (MQ=255)
                                                     tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc          >  1:4215380/1‑69 (MQ=255)
                                                      ccGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg       >  1:3361955/1‑71 (MQ=255)
                                                      ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGcc                                <  1:1361447/46‑1 (MQ=255)
                                                      ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGc          >  1:1502745/1‑68 (MQ=255)
                                                       cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga                     >  1:3009797/1‑56 (MQ=255)
                                                       cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa        <  1:2437817/69‑1 (MQ=255)
                                                         ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag                         <  1:5134430/50‑1 (MQ=255)
                                                          tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg   >  1:2040723/1‑71 (MQ=255)
                                                           aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg   <  1:1520409/70‑1 (MQ=255)
                                                           aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg   <  1:5190734/70‑1 (MQ=255)
                                                             tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc         <  1:4721886/62‑1 (MQ=255)
                                                             tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc  <  1:553044/69‑1 (MQ=255)
                                                             tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc  <  1:395201/69‑1 (MQ=255)
                                                             tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc  <  1:177354/69‑1 (MQ=255)
                                                               cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt            <  1:4706119/57‑1 (MQ=255)
                                                                |                                                                 
TCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGC  >  Exported/2595637‑2595766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: