Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,596,087 C→T 10.4% G171S (GGC→AGC)  arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,596,0870CT10.4% 231.4 / 13.6 86G171S (GGC→AGC) arsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base C (30/47);  new base T (6/3);  total (36/50)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

TAACATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTA  >  Exported/2596016‑2596154
                                                                        |                                                                   
tAACATCACCAGCGT‑‑CAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGcc                                                                     <  1:1656161/70‑1 (MQ=255)
  aCATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGcc                                                                     >  1:5391526/1‑70 (MQ=255)
  aCATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGcc                                                                     <  1:4792270/70‑1 (MQ=255)
  aCATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGcc                                                                     <  1:4053632/70‑1 (MQ=255)
  aCATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGcc                                                                     >  1:2167578/1‑70 (MQ=255)
  aCATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGcc                                                                     >  1:169686/1‑70 (MQ=255)
   cATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGa                                                                   <  1:95430/71‑1 (MQ=255)
    aTCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGa                                                                   <  1:2708317/70‑1 (MQ=255)
       aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGc                                                                >  1:1023456/1‑70 (MQ=255)
       aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGc                                                                >  1:900574/1‑70 (MQ=255)
       aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGc                                                                >  1:3730694/1‑70 (MQ=255)
       aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGc                                                                >  1:1413762/1‑70 (MQ=255)
           gCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCaa                                                             <  1:1658216/69‑1 (MQ=255)
                gCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATc                                                      <  1:4271830/71‑1 (MQ=255)
                   acaaTCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGc                                                   >  1:4656980/1‑71 (MQ=255)
                      aTCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGa                                                <  1:350982/71‑1 (MQ=255)
                         gcggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGaaa                                              <  1:4626850/70‑1 (MQ=255)
                         gcggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGaaa                                              <  1:4620175/70‑1 (MQ=255)
                           ggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGa                                           <  1:1529366/71‑1 (MQ=255)
                             cgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:5208722/1‑71 (MQ=255)
                                tatCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGa                                                <  1:2569275/61‑1 (MQ=255)
                                 atCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCa                                     >  1:3750295/1‑71 (MQ=255)
                                  tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:4246305/1‑66 (MQ=255)
                                  tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:4212338/1‑66 (MQ=255)
                                  tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:3468187/1‑66 (MQ=255)
                                  tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTc                                      >  1:2542745/1‑69 (MQ=255)
                                    cACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGaa                                                         <  1:1592809/48‑1 (MQ=255)
                                    cACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAAt                                                       >  1:3275166/1‑50 (MQ=255)
                                    cACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAcc                                   <  1:4238865/70‑1 (MQ=255)
                                    cACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCa                                  <  1:2734764/71‑1 (MQ=255)
                                     aCCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGaaa                                                        >  1:35870/1‑48 (MQ=255)
                                      ccGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGaa                                                         <  1:5404506/46‑1 (MQ=255)
                                      ccGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAAt                                                       >  1:1408805/1‑48 (MQ=255)
                                      ccGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGa                                                <  1:245251/55‑1 (MQ=255)
                                       cGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGt                               <  1:3055166/71‑1 (MQ=255)
                                        ggCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGaaa                                              >  1:878055/1‑55 (MQ=255)
                                        ggCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                              <  1:2563728/71‑1 (MQ=255)
                                         gCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                             <  1:3655638/71‑1 (MQ=255)
                                          gatcATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGt                               >  1:1748718/4‑68 (MQ=255)
                                            ccATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATc                                                      <  1:2693380/43‑1 (MQ=255)
                                            ccATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATc                                                      <  1:1598296/43‑1 (MQ=255)
                                            ccATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATc                                                      <  1:3048243/43‑1 (MQ=255)
                                            ccATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATc                                                      <  1:642444/43‑1 (MQ=255)
                                            ccATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:3571826/52‑1 (MQ=255)
                                             cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa                         >  1:3207204/1‑69 (MQ=255)
                                             cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa                         >  1:2358490/1‑69 (MQ=255)
                                             cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaa                          >  1:4646796/1‑69 (MQ=255)
                                               tCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:2312640/49‑1 (MQ=255)
                                                 aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                             >  1:467677/1‑63 (MQ=255)
                                                 aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACaa                      >  1:2641538/1‑70 (MQ=255)
                                                 aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                     <  1:1330378/71‑1 (MQ=255)
                                                 aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                     <  1:249723/71‑1 (MQ=255)
                                                   cGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAg                                                    <  1:2248939/38‑1 (MQ=255)
                                                   cGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGa                                           <  1:1179556/47‑1 (MQ=255)
                                                   cGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                     >  1:3443319/1‑69 (MQ=255)
                                                       gCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg               >  1:1004157/1‑71 (MQ=255)
                                                        cGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGc              <  1:4355585/71‑1 (MQ=255)
                                                          tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:2006210/38‑1 (MQ=255)
                                                          tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                            <  1:3508099/39‑1 (MQ=255)
                                                          tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                            <  1:3665311/39‑1 (MQ=255)
                                                          tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                            <  1:5605021/39‑1 (MQ=255)
                                                          tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAg                                 <  1:1461946/50‑1 (MQ=255)
                                                          tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAg                                 <  1:3006230/50‑1 (MQ=255)
                                                          tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGACATTCACCAg                                 <  1:1082108/50‑1 (MQ=255)
                                                           aTTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAgg                                >  1:349147/1‑50 (MQ=255)
                                                           aTTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                    <  1:1196447/62‑1 (MQ=255)
                                                            ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:2061554/36‑1 (MQ=255)
                                                            ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAc                                    <  1:223291/45‑1 (MQ=255)
                                                            ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                             >  1:2173524/1‑52 (MQ=255)
                                                            ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                             >  1:4434754/1‑52 (MQ=255)
                                                            ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGAAATTCACAAGGTTGGAGACAATAAGCGGCAgg           >  1:2493236/1‑70 (MQ=255)
                                                             tCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:3576795/35‑1 (MQ=255)
                                                             tCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:2091012/1‑39 (MQ=255)
                                                             tCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAg                  >  1:5291843/1‑62 (MQ=255)
                                                              cgcgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAc                                    >  1:5292188/1‑43 (MQ=255)
                                                               gcgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         <  1:1122080/37‑1 (MQ=255)
                                                                cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg       >  1:3387305/4‑71 (MQ=255)
                                                                cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg       >  1:3393996/4‑71 (MQ=255)
                                                                cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc      >  1:351590/1‑71 (MQ=255)
                                                                cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc      <  1:3513256/71‑1 (MQ=255)
                                                                cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc      <  1:1877612/71‑1 (MQ=255)
                                                                cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc      >  1:1196194/1‑71 (MQ=255)
                                                                 gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                              <  1:4460923/46‑1 (MQ=255)
                                                                 gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                              <  1:519162/46‑1 (MQ=255)
                                                                 gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCg     <  1:3270281/71‑1 (MQ=255)
                                                                  caaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACa                       <  1:5531407/52‑1 (MQ=255)
                                                                   aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg               <  1:3444343/60‑1 (MQ=255)
                                                                   aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg               <  1:11626/60‑1 (MQ=255)
                                                                   aaaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTTGAGACAATAAGCGGCAGGCTGGCgg    >  1:2759706/1‑71 (MQ=255)
                                                                     aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCa                                  <  1:5092252/39‑1 (MQ=255)
                                                                     aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTa  >  1:4325507/1‑71 (MQ=255)
                                                                      gCCGAGGCCAAATAAATCAGCGGAAACGATATTCACCAGGtt                              <  1:4819783/42‑1 (MQ=255)
                                                                        |                                                                   
TAACATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTA  >  Exported/2596016‑2596154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: