Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,596,088 G→C 10.4% L170L (CTC→CTG arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,596,0880GC10.4% 238.5 / 14.2 86L170L (CTC→CTGarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (30/47);  new base C (6/3);  total (36/50)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

CATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTA  >  Exported/2596019‑2596154
                                                                      |                                                                  
cATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGa                                                                   <  1:95430/71‑1 (MQ=255)
 aTCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGa                                                                   <  1:2708317/70‑1 (MQ=255)
    aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGc                                                                >  1:1023456/1‑70 (MQ=255)
    aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGc                                                                >  1:900574/1‑70 (MQ=255)
    aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGc                                                                >  1:3730694/1‑70 (MQ=255)
    aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGc                                                                >  1:1413762/1‑70 (MQ=255)
        gCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCaa                                                             <  1:1658216/69‑1 (MQ=255)
             gCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATc                                                      <  1:4271830/71‑1 (MQ=255)
                acaaTCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGc                                                   >  1:4656980/1‑71 (MQ=255)
                   aTCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGa                                                <  1:350982/71‑1 (MQ=255)
                      gcggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGaaa                                              <  1:4626850/70‑1 (MQ=255)
                      gcggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGaaa                                              <  1:4620175/70‑1 (MQ=255)
                        ggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGa                                           <  1:1529366/71‑1 (MQ=255)
                          cgATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:5208722/1‑71 (MQ=255)
                             tatCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGa                                                <  1:2569275/61‑1 (MQ=255)
                              atCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCa                                     >  1:3750295/1‑71 (MQ=255)
                               tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:4246305/1‑66 (MQ=255)
                               tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:4212338/1‑66 (MQ=255)
                               tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:3468187/1‑66 (MQ=255)
                               tCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTc                                      >  1:2542745/1‑69 (MQ=255)
                                 cACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGaa                                                         <  1:1592809/48‑1 (MQ=255)
                                 cACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAAt                                                       >  1:3275166/1‑50 (MQ=255)
                                 cACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAcc                                   <  1:4238865/70‑1 (MQ=255)
                                 cACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCa                                  <  1:2734764/71‑1 (MQ=255)
                                  aCCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGaaa                                                        >  1:35870/1‑48 (MQ=255)
                                   ccGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGaa                                                         <  1:5404506/46‑1 (MQ=255)
                                   ccGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAAt                                                       >  1:1408805/1‑48 (MQ=255)
                                   ccGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGa                                                <  1:245251/55‑1 (MQ=255)
                                    cGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGt                               <  1:3055166/71‑1 (MQ=255)
                                     ggCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGaaa                                              >  1:878055/1‑55 (MQ=255)
                                     ggCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                              <  1:2563728/71‑1 (MQ=255)
                                      gCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                             <  1:3655638/71‑1 (MQ=255)
                                       gatcATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGt                               >  1:1748718/4‑68 (MQ=255)
                                         ccATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATc                                                      <  1:3048243/43‑1 (MQ=255)
                                         ccATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATc                                                      <  1:2693380/43‑1 (MQ=255)
                                         ccATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATc                                                      <  1:1598296/43‑1 (MQ=255)
                                         ccATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATc                                                      <  1:642444/43‑1 (MQ=255)
                                         ccATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:3571826/52‑1 (MQ=255)
                                          cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa                         >  1:3207204/1‑69 (MQ=255)
                                          cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa                         >  1:2358490/1‑69 (MQ=255)
                                          cATAACGGAGGCGTACTGCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaa                          >  1:4646796/1‑69 (MQ=255)
                                            tCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:2312640/49‑1 (MQ=255)
                                              aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                             >  1:467677/1‑63 (MQ=255)
                                              aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACaa                      >  1:2641538/1‑70 (MQ=255)
                                              aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                     <  1:1330378/71‑1 (MQ=255)
                                              aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                     <  1:249723/71‑1 (MQ=255)
                                                cGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAg                                                    <  1:2248939/38‑1 (MQ=255)
                                                cGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGa                                           <  1:1179556/47‑1 (MQ=255)
                                                cGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                     >  1:3443319/1‑69 (MQ=255)
                                                    gCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg               >  1:1004157/1‑71 (MQ=255)
                                                     cGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGc              <  1:4355585/71‑1 (MQ=255)
                                                       tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:2006210/38‑1 (MQ=255)
                                                       tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                            <  1:3665311/39‑1 (MQ=255)
                                                       tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                            <  1:5605021/39‑1 (MQ=255)
                                                       tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACg                                            <  1:3508099/39‑1 (MQ=255)
                                                       tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAg                                 <  1:1461946/50‑1 (MQ=255)
                                                       tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAg                                 <  1:3006230/50‑1 (MQ=255)
                                                       tATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGACATTCACCAg                                 <  1:1082108/50‑1 (MQ=255)
                                                        aTTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAgg                                >  1:349147/1‑50 (MQ=255)
                                                        aTTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                    <  1:1196447/62‑1 (MQ=255)
                                                         ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:2061554/36‑1 (MQ=255)
                                                         ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAc                                    <  1:223291/45‑1 (MQ=255)
                                                         ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                             >  1:4434754/1‑52 (MQ=255)
                                                         ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg                             >  1:2173524/1‑52 (MQ=255)
                                                         ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGAAATTCACAAGGTTGGAGACAATAAGCGGCAgg           >  1:2493236/1‑70 (MQ=255)
                                                          tCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAAc                                             <  1:3576795/35‑1 (MQ=255)
                                                          tCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         >  1:2091012/1‑39 (MQ=255)
                                                          tCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAg                  >  1:5291843/1‑62 (MQ=255)
                                                           cgcgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAc                                    >  1:5292188/1‑43 (MQ=255)
                                                            gcgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGata                                         <  1:1122080/37‑1 (MQ=255)
                                                             cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg       >  1:3393996/4‑71 (MQ=255)
                                                             cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg       >  1:3387305/4‑71 (MQ=255)
                                                             cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc      >  1:1196194/1‑71 (MQ=255)
                                                             cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc      <  1:1877612/71‑1 (MQ=255)
                                                             cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc      <  1:3513256/71‑1 (MQ=255)
                                                             cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc      >  1:351590/1‑71 (MQ=255)
                                                              gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                              <  1:519162/46‑1 (MQ=255)
                                                              gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt                              <  1:4460923/46‑1 (MQ=255)
                                                              gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCg     <  1:3270281/71‑1 (MQ=255)
                                                               caaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACa                       <  1:5531407/52‑1 (MQ=255)
                                                                aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg               <  1:11626/60‑1 (MQ=255)
                                                                aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg               <  1:3444343/60‑1 (MQ=255)
                                                                aaaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTTGAGACAATAAGCGGCAGGCTGGCgg    >  1:2759706/1‑71 (MQ=255)
                                                                  aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCa                                  <  1:5092252/39‑1 (MQ=255)
                                                                  aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTa  >  1:4325507/1‑71 (MQ=255)
                                                                   gCCGAGGCCAAATAAATCAGCGGAAACGATATTCACCAGGtt                              <  1:4819783/42‑1 (MQ=255)
                                                                      |                                                                  
CATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTA  >  Exported/2596019‑2596154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: