| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,671,973 | T→A | 5.7% | P316P (CCT→CCA) | malQ → | 4‑alpha‑glucanotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,671,973 | 0 | T | A | 5.7% | 247.9 / 5.6 | 87 | P316P (CCT→CCA) | malQ | 4‑alpha‑glucanotransferase |
| Reads supporting (aligned to +/- strand): ref base T (35/47); new base A (2/3); total (37/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
CTGGCAGGCAGCCTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGTGAT > Exported/2671904‑2672042 | cTGGCAGGCAGCCTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTg > 1:793068/1‑71 (MQ=255) ggcaggcaGCCTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCa > 1:2636489/1‑71 (MQ=255) gcaggcagCCTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGc < 1:2856392/69‑1 (MQ=255) gcaggcagCCTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCa < 1:1737812/70‑1 (MQ=255) aggcagCCTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATgg > 1:5311819/1‑71 (MQ=255) gCCTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCaga < 1:4298832/71‑1 (MQ=255) gCCTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCaga < 1:637659/71‑1 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGc > 1:1421207/1‑59 (MQ=255) tttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGAt > 1:5018773/1‑71 (MQ=255) ttGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATg > 1:4980587/1‑71 (MQ=255) aTGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGt < 1:2742360/69‑1 (MQ=255) aTGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTa > 1:104376/1‑70 (MQ=255) cTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGaa < 1:2055115/71‑1 (MQ=255) cTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGaa < 1:1404534/71‑1 (MQ=255) cTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGaa < 1:1397849/71‑1 (MQ=255) cTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGaa < 1:4466245/71‑1 (MQ=255) aCATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACg > 1:3559507/1‑71 (MQ=255) cATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAAc < 1:1757985/69‑1 (MQ=255) cATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGt < 1:177452/71‑1 (MQ=255) cccAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGAt > 1:5177542/1‑71 (MQ=255) cccAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGAt < 1:431496/71‑1 (MQ=255) aGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCa > 1:1372643/1‑71 (MQ=255) gCAAGTGAAAGAGGATGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCAc > 1:3123422/1‑71 (MQ=255) gCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTg < 1:3309319/64‑1 (MQ=255) gCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCAc > 1:4639577/1‑71 (MQ=255) aaGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGa > 1:396857/1‑64 (MQ=255) aGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAg < 1:1953163/71‑1 (MQ=255) gTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGa < 1:4606244/71‑1 (MQ=255) gTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGa < 1:2139738/71‑1 (MQ=255) gTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGa < 1:3857774/71‑1 (MQ=255) tGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCTCCAGa < 1:3434399/70‑1 (MQ=255) gAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGa < 1:420369/69‑1 (MQ=255) gAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAg > 1:1676323/1‑71 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCaga > 1:4743902/1‑41 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGa > 1:2880622/1‑59 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCAc > 1:4164474/1‑64 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGt > 1:4669209/1‑71 (MQ=255) aaaGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGt < 1:371202/71‑1 (MQ=255) aaGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTg > 1:902273/1‑71 (MQ=255) aaGAGGACGAAATGTGCTGGGGCTGGCCAGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTg > 1:2970342/1‑71 (MQ=255) agagGACGAAATGCGCTGGGGCTGGCCTGCATGGCCaga > 1:3327612/1‑39 (MQ=255) agagGACGAAATGCGCTGGGGCTGGCCTGCATGGCCaga > 1:5290878/1‑39 (MQ=255) agagGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAgaga > 1:1574419/1‑41 (MQ=255) agagGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGc > 1:625487/1‑71 (MQ=255) gagGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAg < 1:564336/48‑1 (MQ=255) gagGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAg < 1:515924/48‑1 (MQ=255) agGACGAAATGCGCTGGGGCTGGCCTGCATGGCCag < 1:5646086/36‑1 (MQ=255) agGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTa < 1:3893495/43‑1 (MQ=255) gACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGTAGTGCg < 1:1143/68‑1 (MQ=255) gACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCg < 1:5678363/68‑1 (MQ=255) gACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCg < 1:2652016/68‑1 (MQ=255) aCGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAg > 1:2344775/1‑71 (MQ=255) aCGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAg < 1:2429295/71‑1 (MQ=255) cGAAATGCGCTGGGGCTGGCCTGCATGGCgagaga > 1:2516993/1‑35 (MQ=255) cGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTa > 1:3955111/1‑39 (MQ=255) gAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGa < 1:3946876/59‑1 (MQ=255) gAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGa < 1:5028982/59‑1 (MQ=255) gAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGa < 1:860454/59‑1 (MQ=255) gAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAg > 1:3456328/1‑61 (MQ=255) aaaTGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTc > 1:3875274/1‑71 (MQ=255) aaaTGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTc > 1:1625921/1‑71 (MQ=255) aaaTGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTc > 1:4120063/1‑71 (MQ=255) aaTGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCt > 1:1588384/1‑71 (MQ=255) aTGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTg > 1:2258647/1‑71 (MQ=255) gcgcTGGGGCTGGCCTGCATGGCCAGAGATGTATCAg < 1:3033769/37‑1 (MQ=255) cgcTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCg < 1:4557092/70‑1 (MQ=255) gcTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCg > 1:484667/1‑69 (MQ=255) tGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTg < 1:3791854/39‑1 (MQ=255) tGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTg < 1:3153380/39‑1 (MQ=255) ggCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACa > 1:2149708/1‑71 (MQ=255) ggCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACa < 1:3128056/71‑1 (MQ=255) gCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGa < 1:1170030/46‑1 (MQ=255) tGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGc < 1:3568678/60‑1 (MQ=255) gCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGaa > 1:2190368/1‑43 (MQ=255) gCCAGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAg < 1:3173040/41‑1 (MQ=255) gCCAGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAg < 1:4624581/41‑1 (MQ=255) gCCAGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAg < 1:5283521/41‑1 (MQ=255) ccTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGtga < 1:1377589/71‑1 (MQ=255) ccTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGtga > 1:825249/1‑71 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGtgat < 1:3301655/71‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGtgat < 1:5653630/71‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGtgat < 1:30590/71‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGtgat < 1:5033466/71‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGtgat < 1:2195791/71‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGtgat < 1:2229612/71‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGtgat < 1:4636406/71‑1 (MQ=255) cTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGtgat < 1:4279359/71‑1 (MQ=255) tGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGa < 1:3467429/39‑1 (MQ=255) tGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAg < 1:4773657/41‑1 (MQ=255) tGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAg < 1:4429355/41‑1 (MQ=255) tGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAAc < 1:5368195/62‑1 (MQ=255) tGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCCTCAGTTCTGCGAAGAACATCGtgat > 1:4126807/1‑70 (MQ=255) | CTGGCAGGCAGCCTTTGATGCGCTACATGCCCAGCAAGTGAAAGAGGACGAAATGCGCTGGGGCTGGCCTGCATGGCCAGAGATGTATCAGAACGTGGATTCACCAGAAGTGCGTCAGTTCTGCGAAGAACATCGTGAT > Exported/2671904‑2672042 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |