Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,677,404	G→A	16.8%	L176L (CTG→TTG)	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,677,404	0	G	A	16.8%	173.5 / 23.9	77	L176L (CTG→TTG)	yhgA	predicted transposase
Reads supporting (aligned to +/- strand):  ref base G (36/28);  new base A (2/11);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATG  >  Exported/2677334‑2677472                                                                       |                                                                     aataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcag                                                                      >  1:383153/1‑71 (MQ=255)  ataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcag                                                                      <  1:1463852/70‑1 (MQ=255)     aGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCagcagc                                                                  >  1:947605/1‑71 (MQ=255)      gCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcg                                                                 <  1:987585/71‑1 (MQ=255)      gCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcg                                                                 <  1:4180252/71‑1 (MQ=255)        aGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcgc                                                                <  1:1133053/70‑1 (MQ=255)          gCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                                                              >  1:652662/1‑70 (MQ=255)            cATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                                                           <  1:4373978/71‑1 (MQ=255)                tGCTCTGCCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATa                                                            >  1:728882/1‑66 (MQ=255)                tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcagca                                                                    >  1:1917869/1‑58 (MQ=255)                tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                                       >  1:1580988/1‑71 (MQ=255)                tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                                       >  1:1750307/1‑71 (MQ=255)                tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                                       >  1:4488858/1‑71 (MQ=255)                tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                                       >  1:5485432/1‑71 (MQ=255)                tGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGAGCCATACGTCg                                                       >  1:4893237/1‑71 (MQ=255)                  ctctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCgg                                                      <  1:1545122/70‑1 (MQ=255)                  ctctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCgg                                                      <  1:422764/70‑1 (MQ=255)                  ctctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCgg                                                      <  1:2703192/70‑1 (MQ=255)                   tctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtg                                                    >  1:140882/1‑71 (MQ=255)                       ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                                 >  1:2235050/1‑70 (MQ=255)                       ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                                 >  1:2848944/1‑70 (MQ=255)                       ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                                 >  1:15778/1‑70 (MQ=255)                        cAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                                                              <  1:5516490/56‑1 (MQ=255)                         aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtg                                                    >  1:4615940/1‑65 (MQ=255)                         aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtg                                                    >  1:2862998/1‑65 (MQ=255)                          atcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAt                                              <  1:2602926/68‑1 (MQ=255)                           tcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAtaa                                            >  1:5350984/2‑69 (MQ=255)                             ccATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAtt                                          <  1:4987377/71‑1 (MQ=255)                             ccATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAtt                                          <  1:1087263/71‑1 (MQ=255)                                tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                                                           <  1:55238/51‑1 (MQ=255)                                tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCa                                               >  1:4551637/1‑63 (MQ=255)                                tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtca                                       >  1:2979993/1‑71 (MQ=255)                                 cAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtca                                       >  1:4320673/1‑70 (MQ=255)                                  aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                      >  1:3072811/1‑70 (MQ=255)                                  aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                      >  1:1993096/1‑70 (MQ=255)                                  aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                                      >  1:3514677/1‑70 (MQ=255)                                    gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                                         <  1:4713278/49‑1 (MQ=255)                                    gTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                                   <  1:2660088/71‑1 (MQ=255)                                       gcgcTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                                 <  1:1036944/70‑2 (MQ=255)                                       gcgcTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                                 <  1:4977092/70‑2 (MQ=255)                                       gcgcTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatcc                                 <  1:4776253/70‑2 (MQ=255)                                       gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCgg                                                      >  1:5460968/1‑49 (MQ=255)                                        cgctGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCgg                               <  1:5353350/68‑1 (MQ=255)                                          ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                                        <  1:886975/44‑1 (MQ=255)                                          ttGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCa                             <  1:5202700/71‑1 (MQ=255)                                           tGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                            >  1:3925169/1‑71 (MQ=255)                                            gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                            >  1:1599291/1‑70 (MQ=255)                                             aCGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                            <  1:921756/69‑1 (MQ=255)                                             aCGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGa                          <  1:5650889/71‑1 (MQ=255)                                               gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACg                        >  1:2702693/1‑71 (MQ=255)                                               gAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAta                                             <  1:3992153/50‑2 (MQ=255)                                               gAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAta                                             <  1:5350784/50‑2 (MQ=255)                                               gAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAta                                             <  1:3023090/50‑2 (MQ=255)                                                aaTATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtg                                                    <  1:2917339/42‑1 (MQ=255)                                                 atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                      >  1:665995/1‑71 (MQ=255)                                                 atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                      >  1:1891867/1‑71 (MQ=255)                                                 atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                      >  1:1906093/1‑71 (MQ=255)                                                 atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                      <  1:2240814/71‑1 (MQ=255)                                                 atatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                      >  1:5192344/1‑71 (MQ=255)                                                  tatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGa                                            >  1:1334354/1‑48 (MQ=255)                                                  tatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGt                      <  1:889489/70‑1 (MQ=255)                                                    tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGAc                   <  1:389629/71‑1 (MQ=255)                                                    tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGAc                   <  1:1823691/71‑1 (MQ=255)                                                    tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGAATGATTTCATCATCAGGCATGACGGTGAc                   <  1:2451865/71‑1 (MQ=255)                                                     gTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACa                  >  1:1055009/1‑71 (MQ=255)                                                      tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAg                                <  1:3642943/56‑1 (MQ=255)                                                      tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAg                                <  1:5547609/56‑1 (MQ=255)                                                       ttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACat                 >  1:2630057/1‑70 (MQ=255)                                                       ttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACat                 >  1:2246251/1‑70 (MQ=255)                                                        tttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatc                <  1:3539959/70‑1 (MQ=255)                                                        tctGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCtcgtcatc                                  >  1:336148/3‑52 (MQ=255)                                                        tctGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCCGCACCACGGTAATatc                <  1:5120915/68‑1 (MQ=255)                                                         ctGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGTAATatcc               <  1:1557292/69‑2 (MQ=255)                                                          tGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaat             >  1:2548112/1‑71 (MQ=255)                                                           gAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGTAATatc                <  1:2017244/67‑1 (MQ=255)                                                            aaTTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaatca           <  1:5275029/71‑1 (MQ=255)                                                               tAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                                   >  1:3982781/1‑44 (MQ=255)                                                                aaCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCg        >  1:4759618/1‑70 (MQ=255)                                                                 aCTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGa      <  1:2503480/71‑1 (MQ=255)                                                                   tCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGaa     >  1:3936979/1‑70 (MQ=255)                                                                    ccAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAAt   >  1:3397653/1‑71 (MQ=255)                                                                     cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGaaa    >  1:4642543/1‑69 (MQ=255)                                                                     cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATg  >  1:391025/1‑71 (MQ=255)                                                                     cagcagCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATg  >  1:3098297/1‑71 (MQ=255)                                                                      agcagcGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATg  >  1:4353244/1‑70 (MQ=255)                                                                       |                                                                     AATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAATCAGCGGAAATG  >  Exported/2677334‑2677472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑