| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,708,493 | C→G | 7.2% | intergenic (+31/‑259) | trpS → / → yhfZ | tryptophanyl‑tRNA synthetase/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,708,493 | 0 | C | G | 7.2% | 210.6 / 4.9 | 70 | intergenic (+31/‑259) | trpS/yhfZ | tryptophanyl‑tRNA synthetase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (32/32); new base G (3/2); total (36/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
GGTGTACGAAGCGATTGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATG > Exported/2708426‑2708557 | ggTGTACGAAGCGATTGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCa > 1:4376342/1‑69 (MQ=255) gtgtACGAAGCGATTGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGc < 1:1940283/71‑1 (MQ=255) tACGAAGCGATTGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCg > 1:1421734/1‑70 (MQ=255) tACGAAGCGATTGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCg < 1:3786150/70‑1 (MQ=255) aaGCGATTGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATgc > 1:3180937/1‑71 (MQ=255) aGCGATTGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATgcg > 1:1250861/1‑71 (MQ=255) cGATTGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATgcggc < 1:4047004/71‑1 (MQ=255) ttGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTg < 1:5413398/71‑1 (MQ=255) ttGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTg < 1:560212/71‑1 (MQ=255) ttGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTg < 1:1736202/71‑1 (MQ=255) ttGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTg < 1:4212794/71‑1 (MQ=255) ttGGTTTTGTGGCGAAGCCGTAATTCACTAATGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTg < 1:2319651/71‑1 (MQ=255) tGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTg > 1:4471182/1‑70 (MQ=255) tGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTg > 1:2688817/1‑70 (MQ=255) ggTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTc > 1:4876917/1‑52 (MQ=255) ttGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGcc > 1:797493/1‑71 (MQ=255) tgtgGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGcc < 1:5037687/70‑1 (MQ=255) gtgGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTg < 1:5219663/63‑1 (MQ=255) tgGCGAAGCCGTAATTCACTATTGCTGGCAAATTTCGCTTTGTTCATGCCgg < 1:363775/52‑1 (MQ=255) tgGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCgg < 1:5222712/52‑1 (MQ=255) tgGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCgg < 1:1200752/52‑1 (MQ=255) tgGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCtt < 1:610389/70‑1 (MQ=255) tgGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa < 1:1204911/71‑1 (MQ=255) tgGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa < 1:736673/71‑1 (MQ=255) tgGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa < 1:186081/71‑1 (MQ=255) tgGAGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCgg < 1:2591235/52‑1 (MQ=255) cGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGt > 1:3838837/1‑58 (MQ=255) cGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCtt < 1:1095803/67‑1 (MQ=255) aaGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa < 1:2277731/66‑1 (MQ=255) ccGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc > 1:4915527/1‑70 (MQ=255) gTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTg < 1:5630881/52‑1 (MQ=255) gTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa > 1:5523793/1‑70 (MQ=255) tAATTCACTATTGCTGGCAAATTGCGCTTTGTTCa > 1:4698067/1‑35 (MQ=255) tAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGtgtg < 1:1891457/51‑1 (MQ=255) tAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc > 1:23368/1‑70 (MQ=255) aTTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATgcggcg > 1:5307026/1‑47 (MQ=255) aTTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa < 1:5000217/67‑1 (MQ=255) ttCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAg < 1:4359624/71‑1 (MQ=255) tCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGaa > 1:2076264/1‑49 (MQ=255) tCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGaa > 1:3076663/1‑49 (MQ=255) cACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGa > 1:1941584/1‑37 (MQ=255) cACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATgcggcg > 1:1441757/1‑44 (MQ=255) aTTGTTGGCAAAGTGTGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTAt > 1:5060043/1‑52 (MQ=11) aTTGTTGGCAAAGTGTGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTAt > 1:3300924/1‑52 (MQ=11) aTTGTTGGCAAAGTGTGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTAt > 1:4534983/1‑52 (MQ=11) ttGCTGGCAAATTGCGCTTTGTTCATGCCGGATgcgg > 1:3865468/1‑37 (MQ=255) ttGCTGGCAAATTGCGCTTTGTTCATGCCGGATgcgg > 1:4583950/1‑37 (MQ=255) ttGCTGGCAAATTGCGCTTTGTTCATGCCGGATgcgg > 1:4387071/1‑37 (MQ=255) ttGCTGGCAAATTGCGCTTTGTTCATGCCGGATgagg > 1:4869840/1‑37 (MQ=255) ttGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCt > 1:5399815/1‑48 (MQ=255) ttGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGc < 1:1674458/70‑1 (MQ=255) ttGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGc < 1:56510/70‑1 (MQ=255) ttGCTGGCAAATTGCGCTTTGTTAATGCCGGATgcgg > 1:3586509/1‑37 (MQ=255) gCTGGCAAATTGCGCTTTGTTCATGCCGGATgcggc > 1:957150/1‑36 (MQ=255) gCTGGCAAATTGCGCTTTGTTCATGCCGGATgcggc > 1:247301/1‑36 (MQ=255) gCTGGCAAATTGCGCTTTGTTCATGCCGGATgcggc > 1:3234032/1‑36 (MQ=255) gCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGcaca > 1:4685801/1‑71 (MQ=255) tGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa < 1:859954/46‑1 (MQ=255) aacAACTTGCGCTTTGTTGATGCCGGATGCGGCGTGAATTCCTTATCCgg < 1:5467278/48‑1 (MQ=11) acttGCGCTTTGTTGATGCCGGATGCGGCGTGAATTCCTTATCCg > 1:3127233/3‑45 (MQ=11) acttGCGCTTTGTTGATGCCGGATGCGGCGTGAATTCCTTATCCg > 1:3592912/3‑45 (MQ=11) acttGCGCTTTGTTGATGCCGGATGCGGCGTGAATTCCTTATCCg > 1:2113157/3‑45 (MQ=11) aaTTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCa < 1:1939108/71‑1 (MQ=255) cttGCGCTTTGTTGATGCCGGATGCGGCGTGAATTCCTTATCCg < 1:2178324/43‑1 (MQ=11) ttGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAg < 1:421498/71‑1 (MQ=255) tGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTaa > 1:580117/1‑55 (MQ=38) gcgcTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAgg > 1:4676054/1‑70 (MQ=255) cgcTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGaa < 1:1651142/71‑1 (MQ=255) cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAAt < 1:3270070/71‑1 (MQ=255) tttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATg > 1:1982313/1‑71 (MQ=255) tGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATg < 1:4888478/69‑1 (MQ=255) | GGTGTACGAAGCGATTGGTTTTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATG > Exported/2708426‑2708557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |