| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,773,653 | C→T | 16.3% | intergenic (+42/‑141) | aceA → / → aceK | isocitrate lyase/isocitrate dehydrogenase kinase/phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,773,653 | 0 | C | T | 16.3% | 136.2 / 16.1 | 61 | intergenic (+42/‑141) | aceA/aceK | isocitrate lyase/isocitrate dehydrogenase kinase/phosphatase |
| Reads supporting (aligned to +/- strand): ref base C (27/24); new base T (4/6); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCC > Exported/2773583‑2773721 | ccGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTc < 1:4093058/71‑1 (MQ=255) cGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTc < 1:4405078/70‑1 (MQ=255) ggCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCAc > 1:2033487/1‑71 (MQ=255) ccACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCAcgccg > 1:2716499/1‑71 (MQ=255) aCTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:3086690/71‑1 (MQ=255) cTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCAcgcc > 1:4812974/1‑67 (MQ=255) gaagaaTCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCa > 1:861432/1‑61 (MQ=255) gaagaaTCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCAcgc > 1:4796055/1‑64 (MQ=255) gaagaaTCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCAcgc > 1:3336804/1‑64 (MQ=255) gaagaaTCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:1321008/1‑70 (MQ=255) gaagaaTCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTACAcgc > 1:5198874/1‑64 (MQ=255) aagaaTCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 1:2625098/1‑71 (MQ=255) aGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCgg < 1:2431421/70‑1 (MQ=255) cTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCa > 1:1625255/1‑47 (MQ=255) cTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCa > 1:2425950/1‑47 (MQ=255) cTAAGCAACAACAACAGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCAc > 1:2642731/1‑71 (MQ=255) gCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑Tg > 1:2082521/1‑71 (MQ=255) gCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑Tg > 1:2750278/1‑71 (MQ=255) gCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑Tg > 1:1488604/1‑71 (MQ=255) gCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑Tg > 1:4513233/1‑71 (MQ=255) gCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑Tg > 1:4473628/1‑71 (MQ=255) aacaacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:1965872/48‑1 (MQ=255) acaacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCa < 1:1562522/47‑1 (MQ=255) acaacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCAc < 1:651087/64‑1 (MQ=255) acaacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCt < 1:551396/71‑1 (MQ=255) caacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCt > 1:5141301/1‑70 (MQ=255) caacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTg < 1:1795518/71‑1 (MQ=255) aacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGa > 1:2894040/1‑71 (MQ=255) aacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGa > 1:3714733/1‑71 (MQ=255) acaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGAt > 1:499976/1‑71 (MQ=255) cTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAt < 1:2311645/43‑1 (MQ=255) gACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTgcg > 1:5337156/1‑71 (MQ=255) cTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGcgcg > 1:4337777/1‑71 (MQ=255) cTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGcgcg < 1:310363/71‑1 (MQ=255) aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCt > 1:4861730/1‑70 (MQ=255) ggCCGGATAAGGCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTa < 1:2980922/70‑1 (MQ=11) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGc < 1:1754404/54‑1 (MQ=255) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCtt < 1:702953/70‑1 (MQ=255) gaccGGATAAGGCGTTCACGCCGCATCCGGCAGTC‑GTGCGCCA‑TGCCTGATGCGACGCTTTGCGCGTCtt < 1:3613819/68‑1 (MQ=1) gaccGGATAAGGCGTTCACGCCGCATCCGGCAGTC‑GTGCGCCA‑TGCCTGATGCGACGCTTTGCGCGTCTTa < 1:895741/69‑1 (MQ=11) gaccGGATAAGGCGTTCACGCCGCATCCGGCAGTC‑GTGCGCCA‑TGCCTGATGCGACGCTTTGCGCGTCTTa < 1:3007464/69‑1 (MQ=11) gaccGGATAAGGCGTTCACGCCGCATCCGGCAGTC‑GTGCGCCA‑TGCCTGATGCGACGCTTTGCGCGTCTTa < 1:3307294/69‑1 (MQ=11) agCCGGATAAGGCGCTCGCGCCGCATCCGGCAA‑CGGTGCCAGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTAt < 1:1428683/70‑1 (MQ=14) gCCGGATAAGGCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTAt < 1:4359086/70‑1 (MQ=11) gCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTAt < 1:1913176/71‑1 (MQ=255) gCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTAt < 1:2817935/71‑1 (MQ=255) accGGATAAGGCGTTCACGCCGCATCCGGCAGTC‑GTGCGCCA‑TGCCTGATGCGACGCTTTGCGCGTCTTAt < 1:1166876/70‑1 (MQ=11) ccGGATAAGGCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTATCa < 1:3793475/71‑1 (MQ=11) ccGGATAAGGCGTTCACGCCGCATCCGGCAGTC‑GTGCGCCA‑TGCCTGATGCGACGCTTTGCGCGTCTTATc < 1:4271156/71‑1 (MQ=11) cGGATAAGGCGCTCGCGCCGCATCCGGCAA‑CGGTGCCAGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCag > 1:1015364/1‑70 (MQ=14) ggATAAGGCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTAt < 1:5187437/67‑1 (MQ=11) ggATAAGGCGTTCACGCCGCATCCGGCAGTC‑GTGCGCCA‑TGCCTGATGCGACGCTTTGCGCGTCTTATCag > 1:455277/1‑70 (MQ=1) aTAAGGCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTATCAGGcc > 1:3814546/1‑71 (MQ=11) aaGGCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTATCAGGCCTa > 1:234268/1‑71 (MQ=11) aGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCt < 1:3737784/70‑1 (MQ=255) ggCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTATCAGGCCTa > 1:3284257/1‑69 (MQ=11) ggCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTATCAGGCCTACa < 1:3713134/71‑1 (MQ=11) ggCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTATCAGGCCTACa < 1:169435/71‑1 (MQ=11) ggCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTAc < 1:423264/71‑1 (MQ=255) cGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTAt > 1:4935700/1‑59 (MQ=11) ttCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGc < 1:214201/40‑1 (MQ=40) ttCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGcc < 1:5093041/71‑1 (MQ=255) ttCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGcc > 1:4729389/1‑71 (MQ=255) | CCGGCTCCACTGAAGAATCGCAGTTCTAAGCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCC > Exported/2773583‑2773721 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |